BBa_K957006
type: ComponentDefinition
persistent identity: BBa K957006
display id: BBa_K957006
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive expression of nah Operon with oriT
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).
owned by: james
owned by: myers
mutable description: One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene. Also included in this BioBrick part is an oriT sequence. When cloned into an existing non-conjugative BioBrick vector, the resulting plasmid may be inserted into a host via conjugation.
discontinued: false
dominant: false
group u list: _1228_
m user id: 0
owner id: 13504
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.
star: false
creator: Dylan Patrick Webster
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J01003
component: BBa_K957005
sequence annotation: BBa_K957005
sequence annotation: BBa_J01003
sequence: https://synbiohub.org/public/igem/BBa_K957006_sequence/1

BBa_J01003
type: Component
persistent identity: component2204252
display id: component2204252
version: 1
top level: BBa_K957006
definition: OriTR
access: public

BBa_K957005
type: Component
persistent identity: component2204251
display id: component2204251
version: 1
top level: BBa_K957006
definition: BBa_K957005
access: public

BBa_K957005
type: SequenceAnnotation
persistent identity: annotation2204251
display id: annotation2204251
version: 1
top level: BBa_K957006
location: https://synbiohub.org/public/igem/BBa_K957006/annotation2204251/range2204251/1
component: BBa_K957005

https://synbiohub.org/public/igem/BBa_K957006/annotation2204251/range2204251/1
type: Range
persistent identity: range2204251
display id: range2204251
version: 1
top level: BBa_K957006
direction: direction/reverse
start: 1
end: 9452
orientation: inline

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation2204252
display id: annotation2204252
version: 1
top level: BBa_K957006
location: https://synbiohub.org/public/igem/BBa_K957006/annotation2204252/range2204252/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_K957006/annotation2204252/range2204252/1
type: Range
persistent identity: range2204252
display id: range2204252
version: 1
top level: BBa_K957006
direction: direction/reverse
start: 9461
end: 9831
orientation: inline

BBa_K957005
type: ComponentDefinition
persistent identity: BBa K957005
display id: BBa_K957005
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive expression of nah Operon for degradation of naphthalene to salicylate
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).
owned by: james
owned by: myers
mutable description: One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.
discontinued: false
dominant: false
experience: experience/None
group u list: _1228_
m user id: 0
owner id: 13504
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.
star: false
creator: Dylan Patrick Webster
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K957004
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K957004
sequence: https://synbiohub.org/public/igem/BBa_K957005_sequence/1

BBa_K957004
type: Component
persistent identity: component2199029
display id: component2199029
version: 1
top level: BBa_K957005
definition: BBa_K957004
access: public

BBa_J23114
type: Component
persistent identity: component2199015
display id: component2199015
version: 1
top level: BBa_K957005
definition: BBa_J23114
access: public

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2199015
display id: annotation2199015
version: 1
top level: BBa_K957005
location: https://synbiohub.org/public/igem/BBa_K957005/annotation2199015/range2199015/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K957005/annotation2199015/range2199015/1
type: Range
persistent identity: range2199015
display id: range2199015
version: 1
top level: BBa_K957005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K957004
type: SequenceAnnotation
persistent identity: annotation2199029
display id: annotation2199029
version: 1
top level: BBa_K957005
location: https://synbiohub.org/public/igem/BBa_K957005/annotation2199029/range2199029/1
component: BBa_K957004

https://synbiohub.org/public/igem/BBa_K957005/annotation2199029/range2199029/1
type: Range
persistent identity: range2199029
display id: range2199029
version: 1
top level: BBa_K957005
direction: direction/reverse
start: 44
end: 9452
orientation: inline

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-R (Origin of transfer for the R-plasmid nic region)
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Golden Bear
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_K957004
type: ComponentDefinition
persistent identity: BBa K957004
display id: BBa_K957004
version: 1
was generated by: iGEM to SBOL conversion
description: nah Operon for degradation of naphthalene to salicylate
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: From NAH7 plasmid of Pseudomonas putida G7 (provided by Dr. Gene Madsen, Cornell University Department of Microbiology).
owned by: james
owned by: myers
mutable description: One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.
discontinued: false
dominant: false
experience: experience/None
group u list: _1228_
m user id: 0
owner id: 13504
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Three internal BioBrick cut sites (one NotI, two PstI) remain in this version of the part. However, the utility of the part is not restricted, since digestion with EcoRI and SpeI facilitates standard molecular cloning.
star: false
creator: Dylan Patrick Webster
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: nahAa
sequence annotation: nahAb
sequence annotation: nahAc
sequence annotation: PstI cut site
sequence annotation: nahAd
sequence annotation: nahB
sequence annotation: nahF
sequence annotation: nahC
sequence annotation: nahQ
sequence annotation: nahE
sequence annotation: NotI cut site
sequence annotation: nahD
sequence: https://synbiohub.org/public/igem/BBa_K957004_sequence/1

nahAa
type: SequenceAnnotation
persistent identity: annotation2199002
display id: annotation2199002
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199002/range2199002/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199002/range2199002/1
type: Range
persistent identity: range2199002
display id: range2199002
version: 1
top level: BBa_K957004
direction: direction/forward
start: 13
end: 999
orientation: inline

nahAb
type: SequenceAnnotation
persistent identity: annotation2199003
display id: annotation2199003
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199003/range2199003/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199003/range2199003/1
type: Range
persistent identity: range2199003
display id: range2199003
version: 1
top level: BBa_K957004
direction: direction/forward
start: 1143
end: 1466
orientation: inline

nahAc
type: SequenceAnnotation
persistent identity: annotation2199004
display id: annotation2199004
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199004/range2199004/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199004/range2199004/1
type: Range
persistent identity: range2199004
display id: range2199004
version: 1
top level: BBa_K957004
direction: direction/forward
start: 1528
end: 2877
orientation: inline

PstI cut site
type: SequenceAnnotation
persistent identity: annotation2199012
display id: annotation2199012
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199012/range2199012/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K957004/annotation2199012/range2199012/1
type: Range
persistent identity: range2199012
display id: range2199012
version: 1
top level: BBa_K957004
direction: direction/forward
start: 1867
end: 1872
orientation: inline

nahAd
type: SequenceAnnotation
persistent identity: annotation2199005
display id: annotation2199005
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199005/range2199005/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199005/range2199005/1
type: Range
persistent identity: range2199005
display id: range2199005
version: 1
top level: BBa_K957004
direction: direction/forward
start: 2892
end: 3476
orientation: inline

nahB
type: SequenceAnnotation
persistent identity: annotation2199006
display id: annotation2199006
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199006/range2199006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199006/range2199006/1
type: Range
persistent identity: range2199006
display id: range2199006
version: 1
top level: BBa_K957004
direction: direction/forward
start: 3546
end: 4325
orientation: inline

nahF
type: SequenceAnnotation
persistent identity: annotation2199007
display id: annotation2199007
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199007/range2199007/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K957004/annotation2199007/range2199007/1
type: Range
persistent identity: range2199007
display id: range2199007
version: 1
top level: BBa_K957004
direction: direction/forward
start: 4371
end: 5822
orientation: inline

nahC
type: SequenceAnnotation
persistent identity: annotation2199008
display id: annotation2199008
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199008/range2199008/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K957004/annotation2199008/range2199008/1
type: Range
persistent identity: range2199008
display id: range2199008
version: 1
top level: BBa_K957004
direction: direction/forward
start: 5849
end: 6757
orientation: inline

PstI cut site
type: SequenceAnnotation
persistent identity: annotation2199013
display id: annotation2199013
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199013/range2199013/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K957004/annotation2199013/range2199013/1
type: Range
persistent identity: range2199013
display id: range2199013
version: 1
top level: BBa_K957004
direction: direction/forward
start: 5862
end: 5867
orientation: inline

nahQ
type: SequenceAnnotation
persistent identity: annotation2199009
display id: annotation2199009
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199009/range2199009/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199009/range2199009/1
type: Range
persistent identity: range2199009
display id: range2199009
version: 1
top level: BBa_K957004
direction: direction/forward
start: 6854
end: 7486
orientation: inline

nahE
type: SequenceAnnotation
persistent identity: annotation2199010
display id: annotation2199010
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199010/range2199010/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199010/range2199010/1
type: Range
persistent identity: range2199010
display id: range2199010
version: 1
top level: BBa_K957004
direction: direction/forward
start: 7535
end: 8530
orientation: inline

NotI cut site
type: SequenceAnnotation
persistent identity: annotation2199014
display id: annotation2199014
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199014/range2199014/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K957004/annotation2199014/range2199014/1
type: Range
persistent identity: range2199014
display id: range2199014
version: 1
top level: BBa_K957004
direction: direction/forward
start: 8165
end: 8172
orientation: inline

nahD
type: SequenceAnnotation
persistent identity: annotation2199011
display id: annotation2199011
version: 1
top level: BBa_K957004
location: https://synbiohub.org/public/igem/BBa_K957004/annotation2199011/range2199011/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K957004/annotation2199011/range2199011/1
type: Range
persistent identity: range2199011
display id: range2199011
version: 1
top level: BBa_K957004
direction: direction/forward
start: 8795
end: 9406
orientation: inline

https://synbiohub.org/public/igem/BBa_K957006_sequence/1
type: Sequence
persistent identity: BBa K957006 sequence
display id: BBa_K957006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagtaagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K957005_sequence/1
type: Sequence
persistent identity: BBa K957005 sequence
display id: BBa_K957005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagtaagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J01003_sequence/1
type: Sequence
persistent identity: BBa J01003 sequence
display id: BBa_J01003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccgccttttcctcaatcgctcttcgttcgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23114_sequence/1
type: Sequence
persistent identity: BBa J23114 sequence
display id: BBa_J23114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K957004_sequence/1
type: Sequence
persistent identity: BBa K957004 sequence
display id: BBa_K957004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgaactgcagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcc…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z