BBa_K957015 1 BBa_K957015 Naphthalene Dioxygenase (From Pseudomonas NAH7 plasmid) 2012-10-27T11:00:00Z 2015-05-08T01:13:49Z Psueomonas putida NAH7 plasmid. Provided by Dr. Eugene L. Madsen, Cornell University Department of Microbiology When the nah operon from the NAH7 Pseudomonas plasmid is heterologously expressed in E. coli, the cells have the ability to synthesize indigo from tryptophan [1]. E. coli contains the enzyme tryptophanase, which splits the indole group from the peptide backbone. Naphthalene dioxygenase, from the nah operon, then creates a compound which will spontaneously eliminate water and be air oxidized into indigo as the final product. [1] Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., & Gibson, D. (1983). Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo. Science, 222(4620), 167-9. false false _1228_ 0 13504 9 Not in stock false Internal PstI cutsite deleted w/ mutagenic PCR. false Dylan Patrick Webster BBa_K957015_sequence 1 taagccggatttatggaacttctcatacagccaaacaatcgcctcattagctttagtcccggcgccaaccttctggaagtgcttcgcgaaaacggtgtcgctatttcctacagttgtatgtctgggcgttgcggaacctgccgctgtcgggttacagatggcagtgtaattgattcgggggcgggaagcgggttaccaaacctcgtggacgagcattatgtgctcgcctgtcagtcagtacttactcacaattgcgcgatcgaaatcccagaaaccgacgaaatcgtcacccacccggcgagaatcatcaagggcactgtggtcgccgtcgagtcgcccactcacgatatccgtcgcctacgcgtacgcctcgctaagcccttcgagttctcacccggacagtacgcgacattgcagttcagtcctgagcatgcgcgtccgtattcaatggcaggtctgccagatgaccaagaaatggagttccacatacgcaaggtgccgggtgggcgcgtaacggagtatgttttcgagcacgtccgcgaaggtacaagcatcaagttgagcgggccacttggtacggcttatttgcgtcagaaccacaccgggccgatgctctgtgtgggcggtgggaccggactagcaccggtgctgtcgattgttcgcggcgcgctgaagttgggtatgacaaaccccatcctcctttatttcggagtgcgcagtcagcaagacctctacgacgcagagcgattgcacaaactcgccgctgatcaccctcaactgaccgtacacacggtaatcgcaatgggcccgattaatgagagtcagcgagccggtctagttaccgatgtgatcgaaaaagacatcatttcgctggctgggtggagggcctacctgtgcggcgcaccagcgatggttgaagcgctttgcaccgttaccaagcatcttggaatatcacccgaacatatttatgccgatgccttctatcccggtggaatctgaatagtccctttccctgcacctctgtccatcgaggactcatcaggaggatactcaaataagcgcaaataataacagtcgcgtcagtatttgcggcagcgaaatggtttccctctccctcatttaccccatctgaggatagttttatgacagaaaaatggattgaagcagtcgccctttctgacatcccagaaggtgatgtcctcggcgtgactgtcgagggcaaggagctggcgttgtacgaagtggaaggcgaaatctacgctaccgacaacctgtgcacgcatggtgccgcccgcatgagcgatggttatctcgaggggcgagaaatcgaatgccccttgcatcaaggtcggtttgacgtttgtacaggcagagccctctgcgcccccgtgacagagaacatcaaaacatatgcagtcaagattgagaacctgcgcgtaatgattgatttgagcggagagttttaataggaggtaccccggaccctagcgcgtaactaccccaattccatcttttttaggtgaaaacatgaattacaaaaacaaaatattggtgagtgaatctgggctgacccaaaagcacctgattcatggcgatgaagaacttttccagcacgaactgagaaccatttttgcgcggaactggctttttctcactcatgacagcctgattccatcccccggcgactatgttaccgcaaaaatgggtattgacgaggtcatcgtctctcggcagagcgacggttcgattcgtgccttcctgaacgtttgtcggcaccgtggcaagacgctggttaacgcggaagccggcaatgccaaaggtttcgtttgcagctatcacggctggggcttcggctccaacggtgagctccagagcgttccattcgaaaaagagctgtacggcgagtcgctcaacaaaaaatgtctggggttgaaagaagtcgctcgcgtggagagcttccatggcttcatctatggttgcttcgatcaggaggcccctcctcttatggactatctgggtgacgctgcttggtacctagagcccatcttcaaacattcaggcggtttagaactggtcggtcctccaggcaaggttgtgatcaaggccaactggaaggcacccgcggaaaactttgtgggggatgcataccacgtgggttggacgcacgcgtcttcgcttcgctcgggagagtctatcttcgcgtcgctcgctggcaacgcagtgctgccccctgaaggtgcaggcttgcaaatgacctccaaatacggcagcggcatgggtgtgttgtgggacggatattcaggcgtgcatagcgcagacttggttccggagttgatggcattcggcggctctaagcaggaaaggctgaacaaagaaattggcgatgttcgcgcccggatttatcgcagccacctcaactgcaccgttttcccgaacaacagcatgctgacctgctcgggtgttttcaaagtatggaacccgatcgacgcaaacaccaccgaggtctggacctacgccattgtcgaaaaagacatgcccgaggatctcaagcgccgcttggccgactcggttcagcgtacgtttgggcctgctggcttctgggaaagcgacgacaatgacaatatggaaacagcgtcgcaaaacggcaagaaatatcaatccagagatagtgatctgctttcaaaccttggtttcggtaaggacgtatacggcgacgcggtctatcctggcgtcgtcggcaaatcggcgatcggcgagaccagttatcgtggtttctaccgggcttaccaggcacacgtcagcagctccaactgggctgagttcgaggatgcctctagtacttggcataccgaactgacgaagactactgatcgctaacagacgagtcgaccatgatgatcaatattcaagaagacaagctggtctccgcccacgacgcccaagagtttcttcgtttcttcaattgccacgacgcggctttgcaacaagaagccaccacgctgctgaaccgggaagcgcatctgttggacattcaggcttaccgggcttggttagagcactgcgtggggtcagaggttcaatatcaggtcatttcacgcgaactgcgcgccgcttccgagcgccgttataagctcaatgaagccatgaacgtttacaacgaaaattttcagcaactgaaagttcgaatcgagcatcaactggatccgcaaaactggagcaacagcccgaagctgcgctttactcgcttcatcaccaatgtccaggccgcaagggacgtagatgacgaagagctacttcacatccgctccaacgtcattctgcaccgggcacgacgtggcaatcaggtcgatgtcttctacgccgcccgggaagacaaatggaaacgtggcgaaggtggagtgcgaaaattggtccagcgattcgtggattacccagagcgcatacttcagacgcacaatctgatggtctttctgtgatccag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z