LOCUS BBa_K976008 5499 bp DNA linear 27-Dec-2024 DEFINITION Yeast ADH1 Promoter + Firefly Luciferase + Yeast ADH1 Promoter + Renilla Luciferase ACCESSION BBa_K976008 VERSION BBa_K976008.1 FEATURES Location/Qualifiers promoter 1..1445 /label=Constitutive promoter misc_feature 1348..1354 /label=TATA Box promoter 1..1445 /label=BBa_J63005 CDS 1452..3104 /label=BBa_I712019 promoter 3113..4557 /label=Constitutive promoter misc_feature 4460..4466 /label=TATA Box promoter 3113..4557 /label=BBa_J63005 misc_feature 4564..5499 /label=Rluc CDS 4564..5499 /label=Rluc misc_feature 4564..5499 /label=BBa_J52008 ORIGIN 1 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa 61 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg 121 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg 181 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat 241 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg 301 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct 361 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt 421 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct 481 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat 541 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt 601 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag 661 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta 721 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc 781 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa 841 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc 901 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact 961 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc 1021 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct 1081 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg 1141 aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt 1201 atcttcgaac acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc 1261 aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt 1321 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg 1381 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat 1441 caacttacta gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc 1501 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc 1561 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact 1621 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca 1681 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat 1741 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca 1801 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt 1861 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg 1921 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta 1981 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga 2041 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg 2101 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga 2161 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga 2221 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc 2281 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca 2341 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg 2401 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca 2461 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg 2521 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg 2581 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga 2641 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat 2701 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc 2761 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca 2821 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg 2881 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag 2941 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg 3001 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag 3061 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaatactag agaagaaatg 3121 atggtaaatg aaataggaaa tcaaggagca tgaaggcaaa agacaaatat aagggtcgaa 3181 cgaaaaataa agtgaaaagt gttgatatga tgtatttggc tttgcggcgc cgaaaaaacg 3241 agtttacgca attgcacaat catgctgact ctgtggcgga cccgcgctct tgccggcccg 3301 gcgataacgc tgggcgtgag gctgtgcccg gcggagtttt ttgcgcctgc attttccaag 3361 gtttaccctg cgctaagggg cgagattgga gaagcaataa gaatgccggt tggggttgcg 3421 atgatgacga ccacgacaac tggtgtcatt atttaagttg ccgaaagaac ctgagtgcat 3481 ttgcaacatg agtatactag aagaatgagc caagacttgc gagacgcgag tttgccggtg 3541 gtgcgaacaa tagagcgacc atgaccttga aggtgagacg cgcataaccg ctagagtact 3601 ttgaagagga aacagcaata gggttgctac cagtataaat agacaggtac atacaacact 3661 ggaaatggtt gtctgtttga gtacgctttc aattcatttg ggtgtgcact ttattatgtt 3721 acaatatgga agggaacttt acacttctcc tatgcacata tattaattaa agtccaatgc 3781 tagtagagaa ggggggtaac acccctccgc gctcttttcc gatttttttc taaaccgtgg 3841 aatatttcgg atatcctttt gttgtttccg ggtgtacaat atggacttcc tcttttctgg 3901 caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct aacatgtagg 3961 tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg gctaaacaag 4021 actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata ctgtagccct 4081 agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt gccatctatt 4141 gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct ctcccccgtt 4201 gttgtctcac catatccgca atgacaaaaa aaatgatgga agacactaaa ggaaaaaatt 4261 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatcttcga 4321 acacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat 4381 acggccttcc ttccagttac ttgaatttga aataaaaaaa gtttgccgct ttgctatcaa 4441 gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct cgttcccttt 4501 cttccttgtt tctttttctg cacaatattt caagctatac caagcataca atcaacttac 4561 tagatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag 4621 tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc 4681 gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg 4741 tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc 4801 ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag 4861 tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac 4921 gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc 4981 atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc 5041 gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac 5101 ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc tgaggagttc 5161 gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac cctctcctgg 5221 cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat tgtccgcaac 5281 tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga gtccgaccct 5341 gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac cgagttcgtg 5401 aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg taagtacatc 5461 aagagcttcg tggagcgcgt gctgaagaac gagcagtaa //