BBa_J63003 1 Kozak & st designed yeast Kozak sequence 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z consensus Kozak and start built from oligonucleotides Released HQ 2013 consensus Kozak sequence and start codon false false _97_ 0 545 97 In stock false designed such that fusion using fusion bricks assembly method leads to in frame translation true Caroline Ajo-Franklin annotation1902852 1 start codon range1902852 1 13 15 BBa_K801011 1 BBa_K801011 TEF1 yeast terminator 2012-09-20T11:00:00Z 2015-05-08T01:13:24Z Extracted from genomic DNA of ''S. cerevisiae'' using PCR. TEF1 terminator of ''S. cervisiae''. false false _1057_ 0 13624 9 In stock false Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional. false Georg Sch??tzinger annotation2202142 1 TEF1-Terminator range2202142 1 1 507 BBa_K124002 1 BBa_K124002 Yeast GPD (TDH3) Promoter 2008-06-30T11:00:00Z 2015-05-08T01:09:44Z Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7 Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter. false false _221_ 0 2512 58 Not in stock false none false John Szymanski BBa_K976000 1 FGFR-1 Fibroblast Growth Factor-1 Receptor Coding Sequence 2013-06-09T11:00:00Z 2015-05-08T01:13:49Z The part has been derived from a genomic sequence provisioned by the Ensembl Database. The Fibroblast Growth Factor-1 Receptor (FGFR-1) is a receptor that is commonly found in melanocytes and is involved with melanocyte differentiation, migration, and division. Malignant melanoma is characterized by abnormally high ligand expression of Fibroblast Growth Factor-2 (FGF2), otherwise known as the Basic Fibroblast Growth Factor (BFGF). The FGFR-1 receptor as a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor. false false _1269_ 0 15167 9 Not in stock false The start codon sequence has been added to the FGFR-1 coding sequence. false Amna Sohail annotation2329945 1 Cytoplasmic Domain range2329945 1 1194 2466 annotation2329993 1 Ig-like C2-type 3 Domain range2329993 1 765 1071 annotation2329943 1 Topological Extracellular Domain range2329943 1 66 1128 annotation2329946 1 Ig-like C2-type 1 Domain range2329946 1 75 357 annotation2329947 1 Ig-like C2-type 2 Domain range2329947 1 474 738 annotation2329994 1 Protein Kinase Domain range2329994 1 1434 2301 annotation2329944 1 Helical Transmembrane Domain range2329944 1 1131 1191 BBa_K976002 1 BBa_K976002 FGFR-1 + FRS2 Complex 2013-06-19T11:00:00Z 2015-05-08T01:13:49Z The FGFR-1 protein sequence has been derived from the Emblem Database. The FRS2 protein sequence has been derived from the UnitProt Database. The Fibroblast Growth Factor Receptor-1 and Fibroblast Growth Factor Receptor Substrate 2 complex comprises a fusion protein complex that has the capability to mediate signal transduction. In melanocytes, this multi-protein complex mediate signal transduction to such pathways as the MAPK pathway, which regulate melanocyte proliferation, migration, and differentiation. false false _1269_ 0 15167 9 Not in stock false No specific design considerations were dealt with. false Amna Sohail component2329800 1 BBa_K976001 component2329799 1 BBa_K976000 annotation2329800 1 BBa_K976001 range2329800 1 2473 3996 annotation2329799 1 BBa_K976000 range2329799 1 1 2466 BBa_K976001 1 FRS2 Fibroblast Growth Factor Receptor Substrate 2 2013-06-19T11:00:00Z 2015-05-08T01:13:49Z Source: UniProt Database Organism: Homo sapiens The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that are involved in melanocyte proliferation, differentiation, and migration. FRS2 may be useful in its ability to mediate downstream signaling as the FGF2 ligand binds to FGFR-1. false false _1269_ 0 15167 9 Not in stock false No particular design considerations were dealt with. false Amna Sohail BBa_K976009 1 BBa_K976009 TDH3 Promoter + Yeast Kozak + FGFR-1/FRS2 Complex +TEF1 Terminator 2013-06-20T11:00:00Z 2015-05-08T01:13:50Z NCBI GenBank.  BioBrick Source & Functions: A. Promoter: BBa_124002; Yeast GPD (TDH3) Promoter Description: The Yeast GPD (TDH3) promoter was designed by John Szymanski of the 2008 Brown Group. This constitutive promoter is still in the planning phase and is 681 base pairs in length. B. Ribosome Binding Site: BBa_J63003; Yeast Kozak Sequence Description: The ribosome binding site was designed by Caroline Ajo-Franklin of the 2006 Silver Lab Group. The BioBrick presents a yeast kozak sequence (ribosome binding site) that is 18 base pairs in length. C. Coding Sequence: BBa_K976000; Fibroblast Growth Factor Receptor-1 Description: The Fibroblast Growth Factor Receptor-1 (FGFR-1) BioBrick has been designed by Amna Sohail and Meredith Gentry of the 2013 MCIT Indianapolis Group. This BioBrick presents a receptor that has a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor. The BioBrick itself is in the planning phase and is 2,466 base pairs in length. D. Coding Sequence: BBa_K976001; Fibroblast Growth Factor Receptor Substrate 2 Description: The Fibroblast Growth Factor Receptor Substrate 2 (FRS2) BioBrick has been designed by Amna Sohail of the 2013 MCIT Indianapolis Group. The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that is involved in melanocyte proliferation, differentiation, and migration. FRS2 may be utilized to mediate downstream signaling to activate luciferase production after the FGF2 ligand binds to FGFR-1. This BioBrick is 1,524 base pairs in length. E. Terminator: BBa_K801011; TEF1 yeast terminator Description: The TEF1 Yeast Terminator was designed by Georg Sch??tzinger of the 2012 TU Munich Group. This BioBrick is derived as a PCR product of the genomic DNA sequence of Saccharomyces cerevisiae and is 507 base pairs in length. false false _1269_ 0 15167 9 Not in stock false None. false Amna Sohail component2329842 1 BBa_K976002 component2329837 1 BBa_K124002 component2329839 1 BBa_J63003 component2329844 1 BBa_K801011 annotation2329842 1 BBa_K976002 range2329842 1 714 4709 annotation2329839 1 BBa_J63003 range2329839 1 690 707 annotation2329837 1 BBa_K124002 range2329837 1 1 681 annotation2329844 1 BBa_K801011 range2329844 1 4718 5224 BBa_K976002_sequence 1 atgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtctccgcggactcctccgcgtccatgaactccggggtcttgttggtccgtccttcccgtttgtcctcctccgggacccctatgttggcgggagtctccgagtatgagttgcctgaggaccctcgttgggagttgcctcgtgaccgtttggtcttggggaaacctttgggggaggggtgttttgggcaagtcgtcttggcggaggcgatcgggttggacaaagacaaacctaaccgtgtcaccaaagtcgcggtcaaaatgttgaaatccgacgcgaccgagaaagacttgtccgacttgatctccgagatggagatgatgaaaatgatcgggaaacataaaaacatcatcaacttgttgggggcgtgtacccaagacgggcctttgtatgtcatcgtcgagtatgcgtccaaagggaacttgcgtgagtatttgcaagcgcgtcgtcctcctgggttggagtattgttataacccttcccataaccctgaggagcaattgtcctccaaagacttagtctcctgtgcgtatcaagtcgcgcgtgggatggagtatttggcgtccaaaaaatgtatccatcgtgacttggcggcgcgtaacgtcttggtcaccgaggacaacgtcatgaaaatcgcggactttgggttggcgcgtgacatccatcatatcgactattataaaaaaaccaccaacgggcgtttgcctgtcaaatggatggcgcctgaggcgttgtttgaccgtatctatacccatcaatccgacgtctggtcctttggggtcttgttgtgggaaatctttaccttgggggggtccccttatcctggggtccctgtcgaggagttgtttaaattgttgaaagaggggcatcgtatggacaaaccttccaactgtaccaacgagttgtatatgatgatgcgtgactgttggcatgcggtcccttcccaacgtcctacctttaaacaattggtcgaggacttggaccgtatcgtcgcgttgacctccaaccaagagtatttggacttgtccatgcctttggaccaatattccccttcctttcctgacacccgttcctccacctgttcctccggggaggactccgtcttttcccatgagcctttgcctgaggagccttgtttgcctcgtcatcctgcgcaattggcgaacggggggttgaaacgtcgttactagatggggtcctgttgttcctgtcctgacaaagacaccgtccctgacaaccatcgtaacaaatttaaagtcatcaacgtcgacgacgacgggaacgagttggggtccgggatcatggagttgaccgacaccgagttgatcttgtatacccgtaaacgtgactccgtcaaatggcattatttgtgtttgcgtcgttatgggtatgactccaacttgttttcctttgagtccgggcgtcgttgtcaaaccgggcaagggatctttgcgtttaaatgtgcgcgtgcggaggagttgtttaacatgttgcaagagatcatgcaaaacaactccatcaacgtcgtcgaggagcctgtcgtcgagcgtaacaaccatcaaaccgagttggaggtccctcgtacccctcgtacccctaccacccctgggtttgcggcgcaaaacttgcctaacgggtatcctcgttatccttcctttggggacgcgtcctcccatccttcctcccgtcatccttccgtcgggtccgcgcgtttgccttccgtcggggaggagtccacccatcctttgttggtcgcggaggagcaagtccatacctatgtcaacaccaccggggtccaagaggagcgtaaaaaccgtacctccgtccatgtccctttggaggcgcgtgtctccaacgcggagtcctccacccctaaagaggagccttcctccatcgaggaccgtgaccctcaaatcttgttggagcctgagggggtcaaatttgtcttggggcctacccctgtccaaaaacaattgatggagaaagagaaattggagcaattggggcgtgaccaagtctccgggtccggggcgaacaacaccgagtgggacaccgggtatgactccgacgagcgtcgtgacgcgccttccgtcaacaaattggtctatgagaacatcaacgggttgtccatcccttccgcgtccggggtccgtcgtgggcgtttgacctccacctccacctccgacacccaaaacatcaacaactccgcgcaacgtcgtaccgcgttgttgaactatgagaacttgccttccttgcctcctgtctgggaggcgcgtaaattgtcccgtgacgaggacgacaacttggggcctaaaaccccttccttgaacgggtatcataacaacttggaccctatgcataactatgtcaacaccgagaacgtcaccgtccctgcgtccgcgcataaaatcgagtattcccgtcgtcgtgactgtacccctaccgtctttaactttgacatccgtcgtccttccttggagcatcgtcaattgaactatatccaagtcgacttggagggggggtccgactccgacaaccctcaaacccctaaaacccctaccacccctttgcctcaaacccctttgcgtcgtaccgagttgtatgcggtcatcgacatcgagcgtaccgcggcgatgtccaacttgcaaaaagcgttgcctcgtgacgacgggacctcccgtaaaacccgtcataactccaccgacttgcctatg BBa_K976001_sequence 1 atggggtcctgttgttcctgtcctgacaaagacaccgtccctgacaaccatcgtaacaaatttaaagtcatcaacgtcgacgacgacgggaacgagttggggtccgggatcatggagttgaccgacaccgagttgatcttgtatacccgtaaacgtgactccgtcaaatggcattatttgtgtttgcgtcgttatgggtatgactccaacttgttttcctttgagtccgggcgtcgttgtcaaaccgggcaagggatctttgcgtttaaatgtgcgcgtgcggaggagttgtttaacatgttgcaagagatcatgcaaaacaactccatcaacgtcgtcgaggagcctgtcgtcgagcgtaacaaccatcaaaccgagttggaggtccctcgtacccctcgtacccctaccacccctgggtttgcggcgcaaaacttgcctaacgggtatcctcgttatccttcctttggggacgcgtcctcccatccttcctcccgtcatccttccgtcgggtccgcgcgtttgccttccgtcggggaggagtccacccatcctttgttggtcgcggaggagcaagtccatacctatgtcaacaccaccggggtccaagaggagcgtaaaaaccgtacctccgtccatgtccctttggaggcgcgtgtctccaacgcggagtcctccacccctaaagaggagccttcctccatcgaggaccgtgaccctcaaatcttgttggagcctgagggggtcaaatttgtcttggggcctacccctgtccaaaaacaattgatggagaaagagaaattggagcaattggggcgtgaccaagtctccgggtccggggcgaacaacaccgagtgggacaccgggtatgactccgacgagcgtcgtgacgcgccttccgtcaacaaattggtctatgagaacatcaacgggttgtccatcccttccgcgtccggggtccgtcgtgggcgtttgacctccacctccacctccgacacccaaaacatcaacaactccgcgcaacgtcgtaccgcgttgttgaactatgagaacttgccttccttgcctcctgtctgggaggcgcgtaaattgtcccgtgacgaggacgacaacttggggcctaaaaccccttccttgaacgggtatcataacaacttggaccctatgcataactatgtcaacaccgagaacgtcaccgtccctgcgtccgcgcataaaatcgagtattcccgtcgtcgtgactgtacccctaccgtctttaactttgacatccgtcgtccttccttggagcatcgtcaattgaactatatccaagtcgacttggagggggggtccgactccgacaaccctcaaacccctaaaacccctaccacccctttgcctcaaacccctttgcgtcgtaccgagttgtatgcggtcatcgacatcgagcgtaccgcggcgatgtccaacttgcaaaaagcgttgcctcgtgacgacgggacctcccgtaaaacccgtcataactccaccgacttgcctatg BBa_K124002_sequence 1 tcagttcgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaa BBa_K976009_sequence 1 tcagttcgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaatactagagcccgccgccaccatggagtactagatgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtctccgcggactcctccgcgtccatgaactccggggtcttgttggtccgtccttcccgtttgtcctcctccgggacccctatgttggcgggagtctccgagtatgagttgcctgaggaccctcgttgggagttgcctcgtgaccgtttggtcttggggaaacctttgggggaggggtgttttgggcaagtcgtcttggcggaggcgatcgggttggacaaagacaaacctaaccgtgtcaccaaagtcgcggtcaaaatgttgaaatccgacgcgaccgagaaagacttgtccgacttgatctccgagatggagatgatgaaaatgatcgggaaacataaaaacatcatcaacttgttgggggcgtgtacccaagacgggcctttgtatgtcatcgtcgagtatgcgtccaaagggaacttgcgtgagtatttgcaagcgcgtcgtcctcctgggttggagtattgttataacccttcccataaccctgaggagcaattgtcctccaaagacttagtctcctgtgcgtatcaagtcgcgcgtgggatggagtatttggcgtccaaaaaatgtatccatcgtgacttggcggcgcgtaacgtcttggtcaccgaggacaacgtcatgaaaatcgcggactttgggttggcgcgtgacatccatcatatcgactattataaaaaaaccaccaacgggcgtttgcctgtcaaatggatggcgcctgaggcgttgtttgaccgtatctatacccatcaatccgacgtctggtcctttggggtcttgttgtgggaaatctttaccttgggggggtccccttatcctggggtccctgtcgaggagttgtttaaattgttgaaagaggggcatcgtatggacaaaccttccaactgtaccaacgagttgtatatgatgatgcgtgactgttggcatgcggtcccttcccaacgtcctacctttaaacaattggtcgaggacttggaccgtatcgtcgcgttgacctccaaccaagagtatttggacttgtccatgcctttggaccaatattccccttcctttcctgacacccgttcctccacctgttcctccggggaggactccgtcttttcccatgagcctttgcctgaggagccttgtttgcctcgtcatcctgcgcaattggcgaacggggggttgaaacgtcgttactagatggggtcctgttgttcctgtcctgacaaagacaccgtccctgacaaccatcgtaacaaatttaaagtcatcaacgtcgacgacgacgggaacgagttggggtccgggatcatggagttgaccgacaccgagttgatcttgtatacccgtaaacgtgactccgtcaaatggcattatttgtgtttgcgtcgttatgggtatgactccaacttgttttcctttgagtccgggcgtcgttgtcaaaccgggcaagggatctttgcgtttaaatgtgcgcgtgcggaggagttgtttaacatgttgcaagagatcatgcaaaacaactccatcaacgtcgtcgaggagcctgtcgtcgagcgtaacaaccatcaaaccgagttggaggtccctcgtacccctcgtacccctaccacccctgggtttgcggcgcaaaacttgcctaacgggtatcctcgttatccttcctttggggacgcgtcctcccatccttcctcccgtcatccttccgtcgggtccgcgcgtttgccttccgtcggggaggagtccacccatcctttgttggtcgcggaggagcaagtccatacctatgtcaacaccaccggggtccaagaggagcgtaaaaaccgtacctccgtccatgtccctttggaggcgcgtgtctccaacgcggagtcctccacccctaaagaggagccttcctccatcgaggaccgtgaccctcaaatcttgttggagcctgagggggtcaaatttgtcttggggcctacccctgtccaaaaacaattgatggagaaagagaaattggagcaattggggcgtgaccaagtctccgggtccggggcgaacaacaccgagtgggacaccgggtatgactccgacgagcgtcgtgacgcgccttccgtcaacaaattggtctatgagaacatcaacgggttgtccatcccttccgcgtccggggtccgtcgtgggcgtttgacctccacctccacctccgacacccaaaacatcaacaactccgcgcaacgtcgtaccgcgttgttgaactatgagaacttgccttccttgcctcctgtctgggaggcgcgtaaattgtcccgtgacgaggacgacaacttggggcctaaaaccccttccttgaacgggtatcataacaacttggaccctatgcataactatgtcaacaccgagaacgtcaccgtccctgcgtccgcgcataaaatcgagtattcccgtcgtcgtgactgtacccctaccgtctttaactttgacatccgtcgtccttccttggagcatcgtcaattgaactatatccaagtcgacttggagggggggtccgactccgacaaccctcaaacccctaaaacccctaccacccctttgcctcaaacccctttgcgtcgtaccgagttgtatgcggtcatcgacatcgagcgtaccgcggcgatgtccaacttgcaaaaagcgttgcctcgtgacgacgggacctcccgtaaaacccgtcataactccaccgacttgcctatgtactagagggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatg BBa_J63003_sequence 1 cccgccgccaccatggag BBa_K976000_sequence 1 atgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtctccgcggactcctccgcgtccatgaactccggggtcttgttggtccgtccttcccgtttgtcctcctccgggacccctatgttggcgggagtctccgagtatgagttgcctgaggaccctcgttgggagttgcctcgtgaccgtttggtcttggggaaacctttgggggaggggtgttttgggcaagtcgtcttggcggaggcgatcgggttggacaaagacaaacctaaccgtgtcaccaaagtcgcggtcaaaatgttgaaatccgacgcgaccgagaaagacttgtccgacttgatctccgagatggagatgatgaaaatgatcgggaaacataaaaacatcatcaacttgttgggggcgtgtacccaagacgggcctttgtatgtcatcgtcgagtatgcgtccaaagggaacttgcgtgagtatttgcaagcgcgtcgtcctcctgggttggagtattgttataacccttcccataaccctgaggagcaattgtcctccaaagacttagtctcctgtgcgtatcaagtcgcgcgtgggatggagtatttggcgtccaaaaaatgtatccatcgtgacttggcggcgcgtaacgtcttggtcaccgaggacaacgtcatgaaaatcgcggactttgggttggcgcgtgacatccatcatatcgactattataaaaaaaccaccaacgggcgtttgcctgtcaaatggatggcgcctgaggcgttgtttgaccgtatctatacccatcaatccgacgtctggtcctttggggtcttgttgtgggaaatctttaccttgggggggtccccttatcctggggtccctgtcgaggagttgtttaaattgttgaaagaggggcatcgtatggacaaaccttccaactgtaccaacgagttgtatatgatgatgcgtgactgttggcatgcggtcccttcccaacgtcctacctttaaacaattggtcgaggacttggaccgtatcgtcgcgttgacctccaaccaagagtatttggacttgtccatgcctttggaccaatattccccttcctttcctgacacccgttcctccacctgttcctccggggaggactccgtcttttcccatgagcctttgcctgaggagccttgtttgcctcgtcatcctgcgcaattggcgaacggggggttgaaacgtcgt BBa_K801011_sequence 1 ggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z