BBa_K976009
type: ComponentDefinition
persistent identity: BBa K976009
display id: BBa_K976009
version: 1
was generated by: iGEM to SBOL conversion
description: TDH3 Promoter + Yeast Kozak + FGFR-1/FRS2 Complex +TEF1 Terminator
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
mutable provenance: NCBI GenBank.
owned by: james
owned by: myers
mutable description:  BioBrick Source & Functions: A. Promoter: BBa_124002; Yeast GPD (TDH3) Promoter Description: The Yeast GPD (TDH3) promoter was designed by John Szymanski of the 2008 Brown Group. This constitutive promoter is still in the planning phase and is 681 base pairs in length. B. Ribosome Binding Site: BBa_J63003; Yeast Kozak Sequence Description: The ribosome binding site was designed by Caroline Ajo-Franklin of the 2006 Silver Lab Group. The BioBrick presents a yeast kozak sequence (ribosome binding site) that is 18 base pairs in length. C. Coding Sequence: BBa_K976000; Fibroblast Growth Factor Receptor-1 Description: The Fibroblast Growth Factor Receptor-1 (FGFR-1) BioBrick has been designed by Amna Sohail and Meredith Gentry of the 2013 MCIT Indianapolis Group. This BioBrick presents a receptor that has a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor. The BioBrick itself is in the planning phase and is 2,466 base pairs in length. D. Coding Sequence: BBa_K976001; Fibroblast Growth Factor Receptor Substrate 2 Description: The Fibroblast Growth Factor Receptor Substrate 2 (FRS2) BioBrick has been designed by Amna Sohail of the 2013 MCIT Indianapolis Group. The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that is involved in melanocyte proliferation, differentiation, and migration. FRS2 may be utilized to mediate downstream signaling to activate luciferase production after the FGF2 ligand binds to FGFR-1. This BioBrick is 1,524 base pairs in length. E. Terminator: BBa_K801011; TEF1 yeast terminator Description: The TEF1 Yeast Terminator…
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Amna Sohail
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K801011
component: BBa_K976002
component: BBa_J63003
component: BBa_K124002
sequence annotation: BBa_K124002
sequence annotation: BBa_J63003
sequence annotation: BBa_K976002
sequence annotation: BBa_K801011
sequence: https://synbiohub.org/public/igem/BBa_K976009_sequence/1

BBa_K801011
type: Component
persistent identity: component2329844
display id: component2329844
version: 1
top level: BBa_K976009
definition: BBa_K801011
access: public

BBa_K976002
type: Component
persistent identity: component2329842
display id: component2329842
version: 1
top level: BBa_K976009
definition: BBa_K976002
access: public

BBa_J63003
type: Component
persistent identity: component2329839
display id: component2329839
version: 1
top level: BBa_K976009
definition: Kozak & st
access: public

BBa_K124002
type: Component
persistent identity: component2329837
display id: component2329837
version: 1
top level: BBa_K976009
definition: BBa_K124002
access: public

BBa_K124002
type: SequenceAnnotation
persistent identity: annotation2329837
display id: annotation2329837
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329837/range2329837/1
component: BBa_K124002

https://synbiohub.org/public/igem/BBa_K976009/annotation2329837/range2329837/1
type: Range
persistent identity: range2329837
display id: range2329837
version: 1
top level: BBa_K976009
direction: direction/reverse
start: 1
end: 681
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2329839
display id: annotation2329839
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329839/range2329839/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_K976009/annotation2329839/range2329839/1
type: Range
persistent identity: range2329839
display id: range2329839
version: 1
top level: BBa_K976009
direction: direction/reverse
start: 690
end: 707
orientation: inline

BBa_K976002
type: SequenceAnnotation
persistent identity: annotation2329842
display id: annotation2329842
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329842/range2329842/1
component: BBa_K976002

https://synbiohub.org/public/igem/BBa_K976009/annotation2329842/range2329842/1
type: Range
persistent identity: range2329842
display id: range2329842
version: 1
top level: BBa_K976009
direction: direction/reverse
start: 714
end: 4709
orientation: inline

BBa_K801011
type: SequenceAnnotation
persistent identity: annotation2329844
display id: annotation2329844
version: 1
top level: BBa_K976009
location: https://synbiohub.org/public/igem/BBa_K976009/annotation2329844/range2329844/1
component: BBa_K801011

https://synbiohub.org/public/igem/BBa_K976009/annotation2329844/range2329844/1
type: Range
persistent identity: range2329844
display id: range2329844
version: 1
top level: BBa_K976009
direction: direction/reverse
start: 4718
end: 5224
orientation: inline

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast GPD (TDH3) Promoter
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: John Szymanski
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

BBa_K976002
type: ComponentDefinition
persistent identity: BBa K976002
display id: BBa_K976002
version: 1
was generated by: iGEM to SBOL conversion
description: FGFR-1 + FRS2 Complex
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: The FGFR-1 protein sequence has been derived from the Emblem Database. The FRS2 protein sequence has been derived from the UnitProt Database.
owned by: james
owned by: myers
mutable description: The Fibroblast Growth Factor Receptor-1 and Fibroblast Growth Factor Receptor Substrate 2 complex comprises a fusion protein complex that has the capability to mediate signal transduction. In melanocytes, this multi-protein complex mediate signal transduction to such pathways as the MAPK pathway, which regulate melanocyte proliferation, migration, and differentiation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No specific design considerations were dealt with.
star: false
creator: Amna Sohail
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K976001
component: BBa_K976000
sequence annotation: BBa_K976000
sequence annotation: BBa_K976001
sequence: https://synbiohub.org/public/igem/BBa_K976002_sequence/1

BBa_K976001
type: Component
persistent identity: component2329800
display id: component2329800
version: 1
top level: BBa_K976002
definition: FRS2
access: public

BBa_K976000
type: Component
persistent identity: component2329799
display id: component2329799
version: 1
top level: BBa_K976002
definition: FGFR-1
access: public

BBa_K976000
type: SequenceAnnotation
persistent identity: annotation2329799
display id: annotation2329799
version: 1
top level: BBa_K976002
location: https://synbiohub.org/public/igem/BBa_K976002/annotation2329799/range2329799/1
component: BBa_K976000

https://synbiohub.org/public/igem/BBa_K976002/annotation2329799/range2329799/1
type: Range
persistent identity: range2329799
display id: range2329799
version: 1
top level: BBa_K976002
direction: direction/reverse
start: 1
end: 2466
orientation: inline

BBa_K976001
type: SequenceAnnotation
persistent identity: annotation2329800
display id: annotation2329800
version: 1
top level: BBa_K976002
location: https://synbiohub.org/public/igem/BBa_K976002/annotation2329800/range2329800/1
component: BBa_K976001

https://synbiohub.org/public/igem/BBa_K976002/annotation2329800/range2329800/1
type: Range
persistent identity: range2329800
display id: range2329800
version: 1
top level: BBa_K976002
direction: direction/reverse
start: 2473
end: 3996
orientation: inline

BBa_K801011
type: ComponentDefinition
persistent identity: BBa K801011
display id: BBa_K801011
version: 1
was generated by: iGEM to SBOL conversion
description: TEF1 yeast terminator
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
owned by: james
owned by: myers
mutable description: TEF1 terminator of ''S. cervisiae''.
discontinued: false
dominant: false
group u list: _1057_
m user id: 0
owner id: 13624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
star: false
creator: Georg Sch??tzinger
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: TEF1-Terminator
sequence: https://synbiohub.org/public/igem/BBa_K801011_sequence/1

TEF1-Terminator
type: SequenceAnnotation
persistent identity: annotation2202142
display id: annotation2202142
version: 1
top level: BBa_K801011
location: https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K801011/annotation2202142/range2202142/1
type: Range
persistent identity: range2202142
display id: range2202142
version: 1
top level: BBa_K801011
direction: direction/forward
start: 1
end: 507
orientation: inline

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: iGEM to SBOL conversion
description: designed yeast Kozak sequence
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: consensus Kozak sequence and start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
star: true
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
top level: Kozak & st
direction: direction/forward
start: 13
end: 15
orientation: inline

FRS2
type: ComponentDefinition
persistent identity: BBa K976001
display id: BBa_K976001
version: 1
was generated by: iGEM to SBOL conversion
description: Fibroblast Growth Factor Receptor Substrate 2
created: 2013-06-19T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: Source: UniProt Database Organism: Homo sapiens
owned by: james
owned by: myers
mutable description: The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that are involved in melanocyte proliferation, differentiation, and migration. FRS2 may be useful in its ability to mediate downstream signaling as the FGF2 ligand binds to FGFR-1.
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No particular design considerations were dealt with.
star: false
creator: Amna Sohail
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K976001_sequence/1

FGFR-1
type: ComponentDefinition
persistent identity: BBa K976000
display id: BBa_K976000
version: 1
was generated by: iGEM to SBOL conversion
description: Fibroblast Growth Factor-1 Receptor Coding Sequence
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:13:49Z
mutable provenance: The part has been derived from a genomic sequence provisioned by the Ensembl Database.
owned by: james
owned by: myers
mutable description: The Fibroblast Growth Factor-1 Receptor (FGFR-1) is a receptor that is commonly found in melanocytes and is involved with melanocyte differentiation, migration, and division. Malignant melanoma is characterized by abnormally high ligand expression of Fibroblast Growth Factor-2 (FGF2), otherwise known as the Basic Fibroblast Growth Factor (BFGF). The FGFR-1 receptor as a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor.
discontinued: false
dominant: false
experience: experience/None
group u list: _1269_
m user id: 0
owner id: 15167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The start codon sequence has been added to the FGFR-1 coding sequence.
star: false
creator: Amna Sohail
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Topological Extracellular Domain
sequence annotation: Ig-like C2-type 1 Domain
sequence annotation: Ig-like C2-type 2 Domain
sequence annotation: Ig-like C2-type 3 Domain
sequence annotation: Helical Transmembrane Domain
sequence annotation: Cytoplasmic Domain
sequence annotation: Protein Kinase Domain
sequence: https://synbiohub.org/public/igem/BBa_K976000_sequence/1

Topological Extracellular Domain
type: SequenceAnnotation
persistent identity: annotation2329943
display id: annotation2329943
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329943/range2329943/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K976000/annotation2329943/range2329943/1
type: Range
persistent identity: range2329943
display id: range2329943
version: 1
top level: FGFR-1
direction: direction/forward
start: 66
end: 1128
orientation: inline

Ig-like C2-type 1 Domain
type: SequenceAnnotation
persistent identity: annotation2329946
display id: annotation2329946
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329946/range2329946/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K976000/annotation2329946/range2329946/1
type: Range
persistent identity: range2329946
display id: range2329946
version: 1
top level: FGFR-1
direction: direction/forward
start: 75
end: 357
orientation: inline

Ig-like C2-type 2 Domain
type: SequenceAnnotation
persistent identity: annotation2329947
display id: annotation2329947
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329947/range2329947/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K976000/annotation2329947/range2329947/1
type: Range
persistent identity: range2329947
display id: range2329947
version: 1
top level: FGFR-1
direction: direction/forward
start: 474
end: 738
orientation: inline

Ig-like C2-type 3 Domain
type: SequenceAnnotation
persistent identity: annotation2329993
display id: annotation2329993
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329993/range2329993/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K976000/annotation2329993/range2329993/1
type: Range
persistent identity: range2329993
display id: range2329993
version: 1
top level: FGFR-1
direction: direction/forward
start: 765
end: 1071
orientation: inline

Helical Transmembrane Domain
type: SequenceAnnotation
persistent identity: annotation2329944
display id: annotation2329944
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329944/range2329944/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K976000/annotation2329944/range2329944/1
type: Range
persistent identity: range2329944
display id: range2329944
version: 1
top level: FGFR-1
direction: direction/forward
start: 1131
end: 1191
orientation: inline

Cytoplasmic Domain
type: SequenceAnnotation
persistent identity: annotation2329945
display id: annotation2329945
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329945/range2329945/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K976000/annotation2329945/range2329945/1
type: Range
persistent identity: range2329945
display id: range2329945
version: 1
top level: FGFR-1
direction: direction/forward
start: 1194
end: 2466
orientation: inline

Protein Kinase Domain
type: SequenceAnnotation
persistent identity: annotation2329994
display id: annotation2329994
version: 1
top level: FGFR-1
location: https://synbiohub.org/public/igem/BBa_K976000/annotation2329994/range2329994/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K976000/annotation2329994/range2329994/1
type: Range
persistent identity: range2329994
display id: range2329994
version: 1
top level: FGFR-1
direction: direction/forward
start: 1434
end: 2301
orientation: inline

https://synbiohub.org/public/igem/BBa_K976009_sequence/1
type: Sequence
persistent identity: BBa K976009 sequence
display id: BBa_K976009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagttcgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaatactagagcccgccgccaccatggagtactagatgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124002_sequence/1
type: Sequence
persistent identity: BBa K124002 sequence
display id: BBa_K124002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagttcgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K976002_sequence/1
type: Sequence
persistent identity: BBa K976002 sequence
display id: BBa_K976002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtctccgcggactcctccgcgtccatgaactccggggtcttgttggtccgtccttcccgtttgtcctcctccgggacccctatgttggcgggagtctccgagtatgagttgcctgaggaccctcgttgggagttgcctcgtgaccgtttggtcttggggaaacctttgggggaggggtgttttgggcaagtcgtcttggcggaggcgatcgggttggacaaagacaaacctaaccgtgtcaccaaagtcgcggtcaaaatgttgaaatccgacgcgaccgagaaagacttgtccgacttgatctccgagatggagatgatgaaaatgatcgggaaacataaaaacatcatcaacttgttgggggcgtgtacccaagacgggcctttgtatgtcatcgtcgagtatgcgtccaaagggaacttgcgtgagtatttgcaagcgcgtcgtcctcctgggttggagtattgttataacccttcccataaccctgaggagcaattgtcctccaaagacttagtctcctgtgcgtatcaagtcgcgcgtgggatggagtatttggcgtccaaaaaatgtatccatcgtgacttggcggcgcgtaacgtcttggtcaccgaggacaacgtcatgaaaatcgcggactttgggttggcgcgtgacatccatcatatcgactattataaaaaaaccaccaacgggcgtttgcctgtcaaatggat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801011_sequence/1
type: Sequence
persistent identity: BBa K801011 sequence
display id: BBa_K801011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctcttttttcagttatatcttaacaggcgacagtccaaatgttgatttatcccagtccgattcatcagggttgtgaagcattttgtcaatggtcgaaatcacatcagtaatagtgcctcttacttgcctcatagaatttctttctcttaacgtcaccgtttgtcttttatagtttcgaaatctatggtgataccaaatggtgttcccaattcatcgttacgggcgtattttttaccaattgaagtattggaatcgtcaattttaaagtatatctctcttttacgtaaagcctgcgagatcctcttaagtatagcggggaagccatcgttattcgatattgtcgtaacaaatactttgagcggcgctatctgtaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63003_sequence/1
type: Sequence
persistent identity: BBa J63003 sequence
display id: BBa_J63003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K976001_sequence/1
type: Sequence
persistent identity: BBa K976001 sequence
display id: BBa_K976001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggggtcctgttgttcctgtcctgacaaagacaccgtccctgacaaccatcgtaacaaatttaaagtcatcaacgtcgacgacgacgggaacgagttggggtccgggatcatggagttgaccgacaccgagttgatcttgtatacccgtaaacgtgactccgtcaaatggcattatttgtgtttgcgtcgttatgggtatgactccaacttgttttcctttgagtccgggcgtcgttgtcaaaccgggcaagggatctttgcgtttaaatgtgcgcgtgcggaggagttgtttaacatgttgcaagagatcatgcaaaacaactccatcaacgtcgtcgaggagcctgtcgtcgagcgtaacaaccatcaaaccgagttggaggtccctcgtacccctcgtacccctaccacccctgggtttgcggcgcaaaacttgcctaacgggtatcctcgttatccttcctttggggacgcgtcctcccatccttcctcccgtcatccttccgtcgggtccgcgcgtttgccttccgtcggggaggagtccacccatcctttgttggtcgcggaggagcaagtccatacctatgtcaacaccaccggggtccaagaggagcgtaaaaaccgtacctccgtccatgtccctttggaggcgcgtgtctccaacgcggagtcctccacccctaaagaggagccttcctccatcgaggaccgtgaccctcaaatcttgttggagcctgagggggtcaaatttgtcttggggcctacccctgtccaaaaacaattgatggagaaagagaaattggagcaattggggcgtgaccaagtctccgggtccggggcgaacaacaccgagtgggacaccgggtatgactccgacgagcgtcgtgacgcgccttccgtcaacaaattggtctatgagaacatcaacgggttgtccatcccttccgcgtccggggtccgtcgtgggcgtttgacctccacctccacctccgacacccaaaacatcaacaactccgcgcaacgtcgtaccgcgttgttgaactatgagaacttgccttccttgcctcctgtctgggaggcgcgtaaattgtcccgtgacgaggacgacaacttggggcctaaaaccccttccttgaacgggtatcataacaacttggaccctatgcataactatgtcaacaccgagaacgtcaccgtccctgcgtccgcgcataaaatcgagtattcccgtcgtcgtgactgtacccctaccgtctttaactttgacatccgtcgtccttccttggagcatcgtcaattgaactatatccaagtcgacttggagggggggtccgactccgacaaccctcaaacccctaaaacccctaccacccctttgcctcaaacccctttgcgtcgtaccgagttgtatgcggtcatcgacatcgagcgtaccgcggcgatgtccaacttgcaaaaagcgttgcctcgtgacgacgggacctcccgtaaaacccgtcataactccaccgacttgcctatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K976000_sequence/1
type: Sequence
persistent identity: BBa K976000 sequence
display id: BBa_K976000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtggtcctggaaatgtttgttgttttgggcggtcttggtcaccgcgaccttgtgtaccgcgcgtccttcccctaccttgcctgagcaagcgcaaccttggggggcgcctgtcgaggtcgagtcctttttggtccatcctggggacttgttgcaattgcgttgtcgtttgcgtgacgacgtccaatccatcaactggttgcgtgacggggtccaattggcggagtccaaccgtacccgtatcaccggggaggaggtcgaggtccaagactccgtccctgcggactccgggttgtatgcgtgtgtcacctcctccccttccgggtccgacaccacctatttttccgtcaacgtctccgacgcgttgccttcctccgaggacgacgacgacgacgacgactcctcctccgaggagaaagaaaccgacaacaccaaacctaaccgtatgcctgtcgcgccttattggacctcccctgagaaaatggagaaaaaattgcatgcggtccctgcggcgaaaaccgtcaaatttaaatgtccttcctccgggacccctaaccctaccttgcgttggttgaaaaacgggaaagagtttaaacctgaccatcgtatcggggggtataaagtccgttatgcgacctggtccatcatcatggactccgtcgtcccttccgacaaagggaactatacctgtatcgtcgagaacgagtatgggtccatcaaccatacctatcaattggacgtcgtcgagcgttcccctcatcgtcctatcttgcaagcggggttgcctgcgaacaaaaccgtcgcgttggggtccaacgtcgagtttatgtgtaaagtctattccgaccctcaacctcatatccaatggttgaaacatatcgaggtcaacgggtccaaaatcgggcctgacaacttgccttatgtccaaatcttgaaaaccgcgggggtcaacaccaccgacaaagagatggaggtcttgcatttgcgtaacgtctcctttgaggacgcgggggagtatacctgtttggcggggaactccatcgggttgtcccatcattccgcgtggttgaccgtcttggaggcgttggaggagcgtcctgcggtcatgacctcccctttgtatttggagatcatcatctattgtaccggggcgtttttgatctcctgtatggtcgagtccgtcatcgtctataaaatgaaatccgggaccaaaaaatccgactttcattcccaaatggcggtccataaattggcgaaatccatccctttgcgtcgtcaagtcaccgtctccgcggactcctccgcgtccatgaactccggggtcttgttggtccgtccttcccgtttgtcctcctccgggacccctatgttggcgggagtctccgagtatgagttgcctgaggaccctcgttgggagttgcctcgtgaccgtttggtcttggggaaacctttgggggaggggtgttttgggcaagtcgtcttggcggaggcgatcgggttggacaaagacaaacctaaccgtgtcaccaaagtcgcggtcaaaatgttgaaatccgacgcgaccgagaaagacttgtccgacttgatctccgagatggagatgatgaaaatgatcgggaaacataaaaacatcatcaacttgttgggggcgtgtacccaagacgggcctttgtatgtcatcgtcgagtatgcgtccaaagggaacttgcgtgagtatttgcaagcgcgtcgtcctcctgggttggagtattgttataacccttcccataaccctgaggagcaattgtcctccaaagacttagtctcctgtgcgtatcaagtcgcgcgtgggatggagtatttggcgtccaaaaaatgtatccatcgtgacttggcggcgcgtaacgtcttggtcaccgaggacaacgtcatgaaaatcgcggactttgggttggcgcgtgacatccatcatatcgactattataaaaaaaccaccaacgggcgtttgcctgtcaaatggat…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z