TDH3 Promoter + Yeast Kozak + FGFR-1/FRS2 Complex +TEF1 Terminator
 BioBrick Source & Functions: A. Promoter: BBa_124002; Yeast GPD (TDH3) Promoter Description: The Yeast GPD (TDH3) promoter was designed by John Szymanski of the 2008 Brown Group. This constitutive promoter is still in the planning phase and is 681 base pairs in length. B. Ribosome Binding Site: BBa_J63003; Yeast Kozak Sequence Description: The ribosome binding site was designed by Caroline Ajo-Franklin of the 2006 Silver Lab Group. The BioBrick presents a yeast kozak sequence (ribosome binding site) that is 18 base pairs in length. C. Coding Sequence: BBa_K976000; Fibroblast Growth Factor Receptor-1 Description: The Fibroblast Growth Factor Receptor-1 (FGFR-1) BioBrick has been designed by Amna Sohail and Meredith Gentry of the 2013 MCIT Indianapolis Group. This BioBrick presents a receptor that has a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor. The BioBrick itself is in the planning phase and is 2,466 base pairs in length. D. Coding Sequence: BBa_K976001; Fibroblast Growth Factor Receptor Substrate 2 Description: The Fibroblast Growth Factor Receptor Substrate 2 (FRS2) BioBrick has been designed by Amna Sohail of the 2013 MCIT Indianapolis Group. The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that is involved in melanocyte proliferation, differentiation, and migration. FRS2 may be utilized to mediate downstream signaling to activate luciferase production after the FGF2 ligand binds to FGFR-1. This BioBrick is 1,524 base pairs in length. E. Terminator: BBa_K801011; TEF1 yeast terminator Description: The TEF1 Yeast Terminator was designed by Georg Sch??tzinger of the 2012 TU Munich Group. This BioBrick is derived as a PCR product of the genomic DNA sequence of Saccharomyces cerevisiae and is 507 base pairs in length.
Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
The FGFR-1 protein sequence has been derived from the Emblem Database. The FRS2 protein sequence has been derived from the UnitProt Database.
The Fibroblast Growth Factor Receptor-1 and Fibroblast Growth Factor Receptor Substrate 2 complex comprises a fusion protein complex that has the capability to mediate signal transduction. In melanocytes, this multi-protein complex mediate signal transduction to such pathways as the MAPK pathway, which regulate melanocyte proliferation, migration, and differentiation.
No specific design considerations were dealt with.
Extracted from genomic DNA of ''S. cerevisiae'' using PCR.
Note: The submitted version of this part has a mutation in the BioBrick suffix: The NdeI restriction site has been lost. However, the part can still be used, because the SpeI and PstI restriction sites are functional.
consensus Kozak and start built from oligonucleotides
consensus Kozak sequence and start codon
designed such that fusion using fusion bricks assembly method leads to in frame translation
Fibroblast Growth Factor Receptor Substrate 2
Source: UniProt Database Organism: Homo sapiens
The Fibroblast Growth Factor Receptor Substrate 2, or FRS2 protein is a lipid based membrane-anchored docking protein that forms a fusion protein complex with the Fibroblast Growth Factor Receptor-1 (FGFR-1) to regulate downstream signal transduction. In melanocytes, this docking protein mediates signal transduction to pathways as the MAPK pathway that are involved in melanocyte proliferation, differentiation, and migration. FRS2 may be useful in its ability to mediate downstream signaling as the FGF2 ligand binds to FGFR-1.
No particular design considerations were dealt with.
Fibroblast Growth Factor-1 Receptor Coding Sequence
The part has been derived from a genomic sequence provisioned by the Ensembl Database.
The Fibroblast Growth Factor-1 Receptor (FGFR-1) is a receptor that is commonly found in melanocytes and is involved with melanocyte differentiation, migration, and division. Malignant melanoma is characterized by abnormally high ligand expression of Fibroblast Growth Factor-2 (FGF2), otherwise known as the Basic Fibroblast Growth Factor (BFGF). The FGFR-1 receptor as a high affinity towards binding to the FGF2 ligand present upon the surface of potentially malignant melanocytes, and therefore may be utilized to detect presence of malignant melanoma tumor.
The start codon sequence has been added to the FGFR-1 coding sequence.
Conversion of the iGEM parts registry to SBOL2.1