BBa_K977000 1 BBa_K977000 Pyruvate Kinase derived from Rabbit Muscle 2013-06-05T11:00:00Z 2015-05-08T01:13:50Z The part was derived from an existing previously constructed plamid in E. coli, carrying the rabbit muscle pyruvate kinase. This part is the 1569 bp long coding sequence of a pyruvate kinase gene variant derived from rabbit muscle (Oryctolagus cuniculus pyruvate kinase, muscle (PKM), transcript variant 1, mRNA) GenBank link - http://www.ncbi.nlm.nih.gov/nucleotide/307548865?report=genbank&log$=nuclalign&blast_rank=1&RID=V0YWBB4E01R Pyruvate kinase is a key regulatory enzyme within glycolysis that removes a phosphate from phosphoenolpyruvate (PEP) adding it to ADP in the production of ATP and pyruvate. The enzyme is allosterically regulated by both inhibitory and excitatory substances. We are attempting to use pyruvate kinase to enhance the downstream metabolic production of alkanes. false false _1270_ 0 10144 9 Not in stock false The original pyruvate kinase gene contained two PstI restriction sites beginning at bp 550 and 908. These restriction sites were removed by either 1) site directed mutagenesis or 2) the purchasing of designed oligos that were stitched together using Gibson Assembly. false Eric Kessler annotation2329786 1 ATG Start Codon range2329786 1 1 3 annotation2329789 1 Site Directed Mutation GCA to GCG range2329789 1 909 911 annotation2329790 1 ATT ATT Stop Codons range2329790 1 1596 1601 annotation2329787 1 Pyruvate Kinase Gene range2329787 1 1 1601 annotation2329788 1 Site Directed Mutation CTG to CTT range2329788 1 549 551 BBa_K977000_sequence 1 tagatgtcaaagtcccacagtgaagccgggtctgccttcatccagacgcagcagctgcacgcagccatggcggacacattcctggagcacatgtgccggctggacatcgactccgcgcccatcacggcccgcaacacgggcatcatctgtaccatcggccctgcctcccgatcggtggagacgctgaaggagatgatcaagtctgggatgaacgtggcgcgcatgaacttctcccacggaactcacgagtaccatgcggagaccatcaagaacgtgcgcacagccacggaaagctttgcctcagaccccatcctctaccggcccgtcgcggtggctctggacactaagggacccgagatccgaacggggctcatcaagggcagcggcacggcggaggtggagctgaagaagggagccacgctcaagatcacgctggacaacgcctacatggagaagtgcgacgagaacatcctgtggctggactacaagaacatttgcaaggtggtggacgtgggcagcaagtctacgtggacgatggcctcatctcccttcaggtgaagcagaaaggtcctgacttcctggtgacggaggtggagaacggtggcttcttgggcagcaagaagggcgtgaacctccccggggctgcggtggacctgcctgccgtgtccgagaaggacatccaggatctgaagttcggcgtggagcaggacgtggacatggtgtttgcctctttcatccgcaaggcggctgacgtccatgaggtcaggaagatcctgggagagaaagggaagaacatcaagatcatcagcaaaattgagaaccacgagggggtgcgcaggtttgatgagatcctggaggccagcgacgggatcatggtggctcgtggtgatcttggcattgagattcctgcggagaaggtcttccttgcccagaagatgataattgggcgatgcaaccgtgctgggaagcctgtcatctgtgccacgcagatgctggagagcatgatcaagaagccccgccccacccgcgccgagggcagtgacgtggccaacgcggtcctggatggagccgactgcatcatgctgtctggagagacggccaagggcgactacccgctggaggccgtccgcatgcagcacctgatagctcgcgaggctgaggcagccatgttccaccgcaagctgttcgaagaacttgcgcgcagctccagtcactccacagacctcatggaggccatggccatgggcagcgtggaggcttcctataagtgtttagcggcagctttgatagttctgacggagtctggcaggtctgctcaccaagtggcccggtaccgcccgcgcgcccccatcattgccgtgacacggaaccaccagaccgcccgccaggcccacctgtaccgtgggatcttcccggtggtgtgtaaggatccggtccaggaggcctgggctgaggatgtcgacctccgggtgaacttggccatgaatgtcggcaaggcccgcggtttcttcaagaagggagatgtggtcattgtgctcaccggatggcgccctggctctggcttcaccaacaccatgcgcgtggtgcccgtcccgattatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z