BBa_K634005 1 BBa_K634005 TagRFP 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Evrogen Red fluorescent protein. May not work see design considerations. false false _809_ 0 9393 9 It's complicated false Maybe lacking necessary 5' elements from original overexpression vector. false Kenneth O. Xu annotation2146644 1 tagRFP range2146644 1 15 750 annotation2129739 1 rbs range2129739 1 1 14 BBa_K985000 1 BBa_K985000 Copper-induced TagRFP Reporter 2013-06-25T11:00:00Z 2015-05-08T01:13:50Z Combination of TagRFP, sara degradation sequence, and Copper-inducible promoter. This part is an assembly of a promoter, a fluorescent protein (brighter than standard GFP), and an ssra degradation tag. It is useful in biosensors to detect the presence of copper ions. Based on a Tinkercell model, the activation of this part can result from as little as 1.3 ppm, and can cause a several micromolar increase in FP production. false false _1282_ 0 16337 9 Not in stock false Please see 2013hs.igem.org/Team:NC_School_of_Sci_Math for in-depth design information. false Jackson Allen component2330114 1 BBa_I760005 component2330116 1 BBa_M0051 component2330119 1 BBa_K634005 annotation2330114 1 BBa_I760005 range2330114 1 1 16 annotation2330119 1 BBa_K634005 range2330119 1 72 821 annotation2330116 1 BBa_M0051 range2330116 1 25 63 BBa_I760005 1 BBa_I760005 Cu-sensitive promoter 2007-08-19T11:00:00Z 2015-08-31T04:08:08Z http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=7804904&from=1054 Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli) false false _148_ 0 2080 9 Not in stock false Sequence from the NCBI database, with added BioBricks prefix and suffix. false Gennady Zakharov BBa_M0051 1 BBa_M0051 AANDENYNYADAS. (Fast) SsrA degradation tag. 2007-12-05T12:00:00Z 2015-05-08T01:13:51Z This variation of the SsrA tag was studied by McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. This sequence codes for the amino acid sequence AANDENYNYADAS, a variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~2 Vmax/ [Clpx6] min-1 from (1). See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240. false false _11_ 0 2398 11 Not in stock false C-terminal tag. Degradation rate is fast. Variations of this sequence yield different degradation rates(see Parts BBa_M0050, BBa_M0052, BBa_M0053). Sequence derived from reverse translation of AANDENYNYADAS sequence using codon usage optimized for E. coli. Three C-terminal aa's (DAS in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system. NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to inlclude them if you use this sequence. false Felix Moser annotation1958881 1 AANDENYNYADAS SsrA deg. tag range1958881 1 1 39 BBa_I760005_sequence 1 atgacaaaattgtcat BBa_K985000_sequence 1 atgacaaaattgtcattactagaggctgctaacgacgaaaactacaactacgctgacgcttcttactagagaggaggaaaaaaaaatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcagaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggccaacaccgagatgctgtaccccgctgacggcggcctggaaggcagaaccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttcaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggaccacagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacaaacttaatggcatggacgagctgtacaagtaat BBa_M0051_sequence 1 gctgctaacgacgaaaactacaactacgctgacgcttct BBa_K634005_sequence 1 aggaggaaaaaaaaatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcagaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggccaacaccgagatgctgtaccccgctgacggcggcctggaaggcagaaccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttcaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggaccacagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacaaacttaatggcatggacgagctgtacaagtaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z