BBa_J31001 1 Hin LVA DNA invertase Hin tagged with LVA 2006-07-11T11:00:00Z 2015-08-31T04:08:45Z Salmonella typhimurium and the the Hin part without LVA. This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter. false false _61_ 0 918 61 In stock true None really false Erin Zwack, Sabriya Rosemond annotation1910565 1 Fwd primer J31001 range1910565 1 1 3 annotation1910566 1 LVA range1910566 1 571 609 annotation1910570 1 Rev primer J31001 range1910570 1 551 612 annotation1910567 1 Stop codon range1910567 1 610 612 annotation1910564 1 Hin range1910564 1 1 570 BBa_K985002 1 BBa_K985002 Phosphate-induced TagBFP2 Reporter 2013-06-25T11:00:00Z 2015-05-08T01:13:50Z Combination of TagBFP2, ssra degradation sequence, and Phosphate-inducible promoter. This part is an assembly of a promoter, a fluorescent protein (brighter than standard GFP), and an ssra degradation tag. It is useful in biosensors to detect the presence of copper ions. Based on a Tinkercell model, the activation of this part can result from as little as 100 ppm, and can cause a several micromolar increase in FP production. false false _1282_ 0 16337 9 Not in stock false Please see 2013hs.igem.org/Team:NC_School_of_Sci_Math false Jackson Allen component2330130 1 BBa_K737026 component2330138 1 BBa_J31001 component2330132 1 BBa_K864100 annotation2330132 1 BBa_K864100 range2330132 1 211 933 annotation2330138 1 BBa_J31001 range2330138 1 940 1551 annotation2330130 1 BBa_K737026 range2330130 1 1 204 BBa_K864100 1 SYFP2 Super Yellow Fluorescent Protein 2 (SYFP2) 2012-09-23T11:00:00Z 2016-01-21T02:29:24Z Amino acid sequence taken from the article by Kremers et al 2006. Released HQ 2013 This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K. false false _1124_ 4206 10137 9 In stock false The sequence has been codon optimized for expression in E coli by DNA 2.0. false Erik Gullberg annotation2196221 1 SYFP2 range2196221 1 1 717 BBa_K737026 1 BBa_K737026 Promoter that can be induced by phosphate and carbon starvation. 2012-09-17T11:00:00Z 2015-05-08T01:13:07Z No The downstream promoter has multiple copies of the pho box, the consensus sequence shared by the pho promoters. These pho boxes are the binding sites of its corresponding response regulators phoB. Two molecules of phoB protein form a complex that could bind to the pho boxes and up-regulate the downstream expression. false false _986_ 0 14291 9 Not in stock false No false Minghao Gong BBa_K864100_sequence 1 atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa BBa_K737026_sequence 1 atgaaacgatgagcaatctgtagagtttgattcagaccttctatattttcccgcttatccgtgccccatctcccattttccctcacccacgccgtcaccgccttgtcatctttctgacaccttactatcttacaaatgtaacaaaaaagttatttttctgtaattcgagcatgtcatgttaccccgcgagcataaaacgcgtga BBa_J31001_sequence 1 atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa BBa_K985002_sequence 1 atgaaacgatgagcaatctgtagagtttgattcagaccttctatattttcccgcttatccgtgccccatctcccattttccctcacccacgccgtcaccgccttgtcatctttctgacaccttactatcttacaaatgtaacaaaaaagttatttttctgtaattcgagcatgtcatgttaccccgcgagcataaaacgcgtgatactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z