LOCUS       BBa_K985003             1447 bp    DNA     linear       22-Dec-2024
DEFINITION  Nitrate-induced SYFP2 reporter
ACCESSION   BBa_K985003
VERSION     BBa_K985003.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     CDS             107..823
                     /label=SYFP2
     CDS             107..829
                     /label=BBa_K864100
     CDS             836..1405
                     /label=Hin
     primer_bind     836..838
                     /label=Fwd primer J31001
     misc_feature    1406..1444
                     /label=LVA
     misc_feature    1445..1447
                     /label=Stop codon
     primer_bind     1386..1447
                     /label=Rev primer J31001
     CDS             836..1447
                     /label=BBa_J31001
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagatgg ttagcaaggg
      121 cgaagaactt tttacaggcg tagtaccgat cttagttgaa ttagacggcg acgttaacgg
      181 tcataagttt agcgtgagcg gtgagggtga aggtgacgca acttacggca agctgaccct
      241 gaagctgatt tgcacgacgg gtaagctgcc ggtcccgtgg cctaccctgg tcacgacctt
      301 gggttatggc gttcagtgtt tcgcgcgtta tccggaccac atgaaacaac acgatttctt
      361 taagagcgcg atgccagaag gctatgtgca ggagcgtacg atctttttca aagacgacgg
      421 taactacaag acgcgtgccg aagtcaaatt cgaaggcgac accctggtga atcgcattga
      481 gctgaagggt attgatttca aagaggatgg caatatcctg ggtcacaagc tggagtacaa
      541 ttacaattcc cacaacgttt acatcaccgc agataaacag aaaaatggca tcaaagcgaa
      601 tttcaaaatc cgtcacaaca ttgaggacgg tggtgttcaa ctggcggatc attaccagca
      661 aaacaccccg attggtgacg gtccggtcct gttgccggat aaccattatc tgtcttacca
      721 aagcaaactg agcaaagatc cgaacgagaa gcgcgaccac atggtgctgc tggagtttgt
      781 gaccgctgcc ggtattaccc tgggtatgga tgagctgtat aaataataat actagatggc
      841 tactattggg tatattcggg tgtcaacaat tgaccaaaat atcgatttac agcgtaatgc
      901 gcttaccagt gcaaattgtg accgcatttt tgaggaccgt atcagtggca agattgcaaa
      961 ccgccccggc ctgaaacgag cgttaaagta tgtaaataaa ggcgatactc ttgtcgtctg
     1021 gaaattagac agactgggcc gcagcgtgaa aaacctggtg gcgttaatat cagaattaca
     1081 tgaacgtgga gctcacttcc attctttaac cgatagtatt gataccagta gcgcgatggg
     1141 gcgattcttt tttcatgtaa tgtcagcact ggccgagatg gagcgagaat taattgtcga
     1201 gcgaaccctt gccggactgg ctgccgccag agcgcaagga cgactgggag ggcgccctcg
     1261 ggcgatcaac agacatgaac aggaacagat tagtcggcta ttagagaaag gccatcctcg
     1321 gcagcaacta gctattattt ttggtattgg cgtatctacc ttatacagat attttccggc
     1381 aagccgtata aaaaaacgaa tgaataggcc tgctgcaaac gacgaaaact acgctttagt
     1441 agcttaa
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