LOCUS       BBa_K986002             2253 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737026+S05115
ACCESSION   BBa_K986002
VERSION     BBa_K986002.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..225
                     /label=RBS-3\Weak
     RBS             219..222
                     /label=RBS
     misc_feature    213..225
                     /label=BBa_B0032
     RBS             213..225
                     /label=BBa_B0032
     misc_feature    1321..1359
                     /label=LVA
     CDS             232..1359
                     /label=lacI-LVA
     misc_feature    232..1359
                     /label=BBa_C0012
     misc_feature    1360..1384
                     /label=Help:Barcodes
     CDS             232..1359
                     /label=BBa_C0012
     misc_feature    213..1384
                     /label=BBa_S05101
     misc_feature    1397..1400
                     /label=conserved
     RBS             1393..1404
                     /label=BBa_B0034
     CDS             1411..2085
                     /label=mrfp1
     misc_feature    2092..2116
                     /label=Help:Barcodes
     CDS             1411..2091
                     /label=BBa_E1010
     stem_loop       2136..2179
                     /label=stem_loop
     misc_feature    2125..2204
                     /label=BBa_B0010
     terminator      2125..2204
                     /label=BBa_B0010
     stem_loop       2220..2239
                     /label=T7 TE
     misc_feature    2246..2246
                     /label=stop
     polyA_site      2240..2253
                     /label=polya
     misc_feature    2213..2253
                     /label=BBa_B0012
     terminator      2213..2253
                     /label=BBa_B0012
     misc_feature    1393..2253
                     /label=BBa_I13507
     misc_feature    213..2253
                     /label=BBa_S05115
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agtcacacag gaaagtacta gatggtgaat
      241 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
      301 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      361 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      421 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      481 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      541 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      601 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      661 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      721 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      781 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      841 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      901 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      961 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     1021 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     1081 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     1141 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     1201 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1261 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1321 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
     1381 tctctactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
     1441 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
     1501 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
     1561 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1621 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1681 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1741 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1801 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1861 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1921 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1981 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     2041 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     2101 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2161 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2221 gctcaccttc gggtgggcct ttctgcgttt ata
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