LOCUS       BBa_K986003             2252 bp    DNA     linear       01-Jul-2024
DEFINITION  BBa_K737026+S05117
ACCESSION   BBa_K986003
VERSION     BBa_K986003.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..224
                     /label=BBa_J61117
     misc_feature    1320..1358
                     /label=LVA
     CDS             231..1358
                     /label=lacI-LVA
     misc_feature    231..1358
                     /label=BBa_C0012
     misc_feature    1359..1383
                     /label=Help:Barcodes
     CDS             231..1358
                     /label=BBa_C0012
     misc_feature    213..1383
                     /label=BBa_S05104
     misc_feature    1396..1399
                     /label=conserved
     RBS             1392..1403
                     /label=BBa_B0034
     CDS             1410..2084
                     /label=mrfp1
     misc_feature    2091..2115
                     /label=Help:Barcodes
     CDS             1410..2090
                     /label=BBa_E1010
     stem_loop       2135..2178
                     /label=stem_loop
     misc_feature    2124..2203
                     /label=BBa_B0010
     terminator      2124..2203
                     /label=BBa_B0010
     stem_loop       2219..2238
                     /label=T7 TE
     misc_feature    2245..2245
                     /label=stop
     polyA_site      2239..2252
                     /label=polya
     misc_feature    2212..2252
                     /label=BBa_B0012
     terminator      2212..2252
                     /label=BBa_B0012
     misc_feature    1392..2252
                     /label=BBa_I13507
     misc_feature    213..2252
                     /label=BBa_S05117
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agaaagacat gagttactag atggtgaatg
      241 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
      301 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
      361 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
      421 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
      481 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
      541 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
      601 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
      661 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
      721 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
      781 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
      841 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
      901 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
      961 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     1021 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     1081 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     1141 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     1201 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     1261 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg
     1321 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
     1381 ctctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     1441 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     1501 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     1561 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     1621 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     1681 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     1741 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     1801 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     1861 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     1921 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     1981 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     2041 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     2101 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2161 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2221 ctcaccttcg ggtgggcctt tctgcgttta ta
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