LOCUS       BBa_K986004             1784 bp    DNA     linear       23-Dec-2024
DEFINITION  BBa_K737026+BBa_S05118
ACCESSION   BBa_K986004
VERSION     BBa_K986004.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..224
                     /label=BBa_J61117
     CDS             234..850
                     /label=tetR
     misc_feature    851..884
                     /label=SsrA
     misc_feature    891..915
                     /label=Help:Barcodes
     CDS             231..915
                     /label=BBa_C0040
     misc_feature    213..915
                     /label=BBa_S05105
     misc_feature    928..931
                     /label=conserved
     RBS             924..935
                     /label=BBa_B0034
     CDS             942..1616
                     /label=mrfp1
     misc_feature    1623..1647
                     /label=Help:Barcodes
     CDS             942..1622
                     /label=BBa_E1010
     stem_loop       1667..1710
                     /label=stem_loop
     misc_feature    1656..1735
                     /label=BBa_B0010
     terminator      1656..1735
                     /label=BBa_B0010
     stem_loop       1751..1770
                     /label=T7 TE
     misc_feature    1777..1777
                     /label=stop
     polyA_site      1771..1784
                     /label=polya
     misc_feature    1744..1784
                     /label=BBa_B0012
     terminator      1744..1784
                     /label=BBa_B0012
     misc_feature    924..1784
                     /label=BBa_I13507
     misc_feature    213..1784
                     /label=BBa_S05118
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agaaagacat gagttactag atgtccagat
      241 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      301 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      361 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      421 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      481 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      541 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      601 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
      661 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      721 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
      781 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
      841 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt
      901 gctagtgtag atcactacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      961 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
     1021 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
     1081 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     1141 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     1201 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     1261 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1321 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1381 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1441 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1501 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1561 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1621 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     1681 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1741 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//