LOCUS BBa_K986005 2252 bp DNA linear 23-Dec-2024 DEFINITION BBa_K737026+BBa_S05119 ACCESSION BBa_K986005 VERSION BBa_K986005.1 FEATURES Location/Qualifiers promoter 1..204 /label=BBa_K737026 RBS 213..224 /label=BBa_J61101 misc_feature 1320..1358 /label=LVA CDS 231..1358 /label=lacI-LVA misc_feature 231..1358 /label=BBa_C0012 misc_feature 1359..1383 /label=Help:Barcodes CDS 231..1358 /label=BBa_C0012 misc_feature 213..1383 /label=BBa_S05107 misc_feature 1396..1399 /label=conserved RBS 1392..1403 /label=BBa_B0034 CDS 1410..2084 /label=mrfp1 misc_feature 2091..2115 /label=Help:Barcodes CDS 1410..2090 /label=BBa_E1010 stem_loop 2135..2178 /label=stem_loop misc_feature 2124..2203 /label=BBa_B0010 terminator 2124..2203 /label=BBa_B0010 stem_loop 2219..2238 /label=T7 TE misc_feature 2245..2245 /label=stop polyA_site 2239..2252 /label=polya misc_feature 2212..2252 /label=BBa_B0012 terminator 2212..2252 /label=BBa_B0012 misc_feature 1392..2252 /label=BBa_I13507 misc_feature 213..2252 /label=BBa_S05119 ORIGIN 1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc 61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac 121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt 181 accccgcgag cataaaacgc gtgatactag agaaagacag gacctactag atggtgaatg 241 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 301 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 361 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 421 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 481 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 541 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 601 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 661 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 721 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 781 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 841 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 901 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 961 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 1021 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 1081 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 1141 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 1201 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 1261 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg 1321 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 1381 ctctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt 1441 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag 1501 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag 1561 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag 1621 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt 1681 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact 1741 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt 1801 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt 1861 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg 1921 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg 1981 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg 2041 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt 2101 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2161 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2221 ctcaccttcg ggtgggcctt tctgcgttta ta //