LOCUS       BBa_K986007             2141 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737023+BBa_S05115
ACCESSION   BBa_K986007
VERSION     BBa_K986007.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..113
                     /label=RBS-3\Weak
     RBS             107..110
                     /label=RBS
     misc_feature    101..113
                     /label=BBa_B0032
     RBS             101..113
                     /label=BBa_B0032
     misc_feature    1209..1247
                     /label=LVA
     CDS             120..1247
                     /label=lacI-LVA
     misc_feature    120..1247
                     /label=BBa_C0012
     misc_feature    1248..1272
                     /label=Help:Barcodes
     CDS             120..1247
                     /label=BBa_C0012
     misc_feature    101..1272
                     /label=BBa_S05101
     misc_feature    1285..1288
                     /label=conserved
     RBS             1281..1292
                     /label=BBa_B0034
     CDS             1299..1973
                     /label=mrfp1
     misc_feature    1980..2004
                     /label=Help:Barcodes
     CDS             1299..1979
                     /label=BBa_E1010
     stem_loop       2024..2067
                     /label=stem_loop
     misc_feature    2013..2092
                     /label=BBa_B0010
     terminator      2013..2092
                     /label=BBa_B0010
     stem_loop       2108..2127
                     /label=T7 TE
     misc_feature    2134..2134
                     /label=stop
     polyA_site      2128..2141
                     /label=polya
     misc_feature    2101..2141
                     /label=BBa_B0012
     terminator      2101..2141
                     /label=BBa_B0012
     misc_feature    1281..2141
                     /label=BBa_I13507
     misc_feature    101..2141
                     /label=BBa_S05115
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga
      121 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      181 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      301 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
      361 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
      421 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
      481 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
      541 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
      601 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
      661 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
      721 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
      781 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
      841 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
      901 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
      961 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     1021 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     1081 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct
     1261 agtgtagatc tctactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
     1321 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     1381 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     1441 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1501 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1561 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     1621 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1681 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     1741 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     1801 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     1861 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     1921 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
     1981 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     2041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2101 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//