LOCUS       BBa_K986009             1672 bp    DNA     linear       01-Jul-2024
DEFINITION  BBa_K737023+S05118
ACCESSION   BBa_K986009
VERSION     BBa_K986009.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..112
                     /label=BBa_J61117
     CDS             122..738
                     /label=tetR
     misc_feature    739..772
                     /label=SsrA
     misc_feature    779..803
                     /label=Help:Barcodes
     CDS             119..803
                     /label=BBa_C0040
     misc_feature    101..803
                     /label=BBa_S05105
     misc_feature    816..819
                     /label=conserved
     RBS             812..823
                     /label=BBa_B0034
     CDS             830..1504
                     /label=mrfp1
     misc_feature    1511..1535
                     /label=Help:Barcodes
     CDS             830..1510
                     /label=BBa_E1010
     stem_loop       1555..1598
                     /label=stem_loop
     misc_feature    1544..1623
                     /label=BBa_B0010
     terminator      1544..1623
                     /label=BBa_B0010
     stem_loop       1639..1658
                     /label=T7 TE
     misc_feature    1665..1665
                     /label=stop
     polyA_site      1659..1672
                     /label=polya
     misc_feature    1632..1672
                     /label=BBa_B0012
     terminator      1632..1672
                     /label=BBa_B0012
     misc_feature    812..1672
                     /label=BBa_I13507
     misc_feature    101..1672
                     /label=BBa_S05118
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacatga gttactagat
      121 gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg
      181 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt
      241 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga
      301 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa
      361 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt
      421 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg
      481 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac
      541 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc
      601 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg
      661 tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact
      721 taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca
      781 ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga tggcttcctc
      841 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      901 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      961 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     1021 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     1081 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     1141 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     1201 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     1261 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     1321 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     1381 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1441 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1501 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1621 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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