LOCUS       BBa_K986010             2140 bp    DNA     linear       22-Nov-2024
DEFINITION  BBa_K737023+BBa_S05119
ACCESSION   BBa_K986010
VERSION     BBa_K986010.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..112
                     /label=BBa_J61101
     misc_feature    1208..1246
                     /label=LVA
     CDS             119..1246
                     /label=lacI-LVA
     misc_feature    119..1246
                     /label=BBa_C0012
     misc_feature    1247..1271
                     /label=Help:Barcodes
     CDS             119..1246
                     /label=BBa_C0012
     misc_feature    101..1271
                     /label=BBa_S05107
     misc_feature    1284..1287
                     /label=conserved
     RBS             1280..1291
                     /label=BBa_B0034
     CDS             1298..1972
                     /label=mrfp1
     misc_feature    1979..2003
                     /label=Help:Barcodes
     CDS             1298..1978
                     /label=BBa_E1010
     stem_loop       2023..2066
                     /label=stem_loop
     misc_feature    2012..2091
                     /label=BBa_B0010
     terminator      2012..2091
                     /label=BBa_B0010
     stem_loop       2107..2126
                     /label=T7 TE
     misc_feature    2133..2133
                     /label=stop
     polyA_site      2127..2140
                     /label=polya
     misc_feature    2100..2140
                     /label=BBa_B0012
     terminator      2100..2140
                     /label=BBa_B0012
     misc_feature    1280..2140
                     /label=BBa_I13507
     misc_feature    101..2140
                     /label=BBa_S05119
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacagga cctactagat
      121 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
      181 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      241 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      301 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
      361 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
      421 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
      481 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
      541 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      601 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      661 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      721 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      781 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
      841 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
      901 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
      961 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     1021 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1081 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1201 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta
     1261 gtgtagatct ctactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
     1321 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1381 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1441 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1501 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1561 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1621 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1681 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1741 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1801 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1861 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1921 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
     1981 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     2041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2101 cacactggct caccttcggg tgggcctttc tgcgtttata
//