LOCUS       BBa_K986012             1792 bp    DNA     linear       23-Dec-2024
DEFINITION  BBa_K737026+BBa_S05124
ACCESSION   BBa_K986012
VERSION     BBa_K986012.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..224
                     /label=BBa_J61117
     CDS             234..850
                     /label=tetR
     misc_feature    851..884
                     /label=SsrA
     misc_feature    891..915
                     /label=Help:Barcodes
     CDS             231..915
                     /label=BBa_C0040
     misc_feature    213..915
                     /label=BBa_S05105
     misc_feature    928..931
                     /label=conserved
     RBS             924..935
                     /label=BBa_B0034
     CDS             942..1652
                     /label=mCherry
     misc_feature    1293..1293
                     /label=C->T (removing PstI site)
     misc_feature    942..1655
                     /label=BBa_J06504
     stem_loop       1675..1718
                     /label=stem_loop
     misc_feature    1664..1743
                     /label=BBa_B0010
     terminator      1664..1743
                     /label=BBa_B0010
     stem_loop       1759..1778
                     /label=T7 TE
     misc_feature    1785..1785
                     /label=stop
     polyA_site      1779..1792
                     /label=polya
     misc_feature    1752..1792
                     /label=BBa_B0012
     terminator      1752..1792
                     /label=BBa_B0012
     misc_feature    924..1792
                     /label=BBa_J06702
     misc_feature    213..1792
                     /label=BBa_S05124
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agaaagacat gagttactag atgtccagat
      241 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      301 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      361 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      421 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      481 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      541 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      601 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
      661 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      721 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
      781 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
      841 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt
      901 gctagtgtag atcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg
      961 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1021 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1081 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1141 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1201 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1261 gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg
     1321 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1381 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1441 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     1501 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     1561 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     1621 ccaccggcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa
     1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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