LOCUS BBa_K986012 1792 bp DNA linear 23-Nov-2024 DEFINITION BBa_K737026+BBa_S05124 ACCESSION BBa_K986012 VERSION BBa_K986012.1 FEATURES Location/Qualifiers promoter 1..204 /label=BBa_K737026 RBS 213..224 /label=BBa_J61117 CDS 234..850 /label=tetR misc_feature 851..884 /label=SsrA misc_feature 891..915 /label=Help:Barcodes CDS 231..915 /label=BBa_C0040 misc_feature 213..915 /label=BBa_S05105 misc_feature 928..931 /label=conserved RBS 924..935 /label=BBa_B0034 CDS 942..1652 /label=mCherry misc_feature 1293..1293 /label=C->T (removing PstI site) misc_feature 942..1655 /label=BBa_J06504 stem_loop 1675..1718 /label=stem_loop misc_feature 1664..1743 /label=BBa_B0010 terminator 1664..1743 /label=BBa_B0010 stem_loop 1759..1778 /label=T7 TE misc_feature 1785..1785 /label=stop polyA_site 1779..1792 /label=polya misc_feature 1752..1792 /label=BBa_B0012 terminator 1752..1792 /label=BBa_B0012 misc_feature 924..1792 /label=BBa_J06702 misc_feature 213..1792 /label=BBa_S05124 ORIGIN 1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc 61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac 121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt 181 accccgcgag cataaaacgc gtgatactag agaaagacat gagttactag atgtccagat 241 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag 301 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc 361 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc 421 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta 481 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac 541 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag 601 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt 661 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg 721 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc 781 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg 841 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt 901 gctagtgtag atcactacta gagaaagagg agaaatacta gatggtgagc aagggcgagg 961 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 1021 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 1081 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 1141 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 1201 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 1261 gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg 1321 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 1381 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 1441 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 1501 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 1561 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 1621 ccaccggcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa 1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //