LOCUS       BBa_K986013             2260 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737026+BBa_S05123
ACCESSION   BBa_K986013
VERSION     BBa_K986013.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..224
                     /label=BBa_J61117
     misc_feature    1320..1358
                     /label=LVA
     CDS             231..1358
                     /label=lacI-LVA
     misc_feature    231..1358
                     /label=BBa_C0012
     misc_feature    1359..1383
                     /label=Help:Barcodes
     CDS             231..1358
                     /label=BBa_C0012
     misc_feature    213..1383
                     /label=BBa_S05104
     misc_feature    1396..1399
                     /label=conserved
     RBS             1392..1403
                     /label=BBa_B0034
     CDS             1410..2120
                     /label=mCherry
     misc_feature    1761..1761
                     /label=C->T (removing PstI site)
     misc_feature    1410..2123
                     /label=BBa_J06504
     stem_loop       2143..2186
                     /label=stem_loop
     misc_feature    2132..2211
                     /label=BBa_B0010
     terminator      2132..2211
                     /label=BBa_B0010
     stem_loop       2227..2246
                     /label=T7 TE
     misc_feature    2253..2253
                     /label=stop
     polyA_site      2247..2260
                     /label=polya
     misc_feature    2220..2260
                     /label=BBa_B0012
     terminator      2220..2260
                     /label=BBa_B0012
     misc_feature    1392..2260
                     /label=BBa_J06702
     misc_feature    213..2260
                     /label=BBa_S05123
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agaaagacat gagttactag atggtgaatg
      241 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
      301 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
      361 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
      421 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
      481 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
      541 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
      601 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
      661 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
      721 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
      781 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
      841 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
      901 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
      961 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     1021 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     1081 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     1141 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     1201 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     1261 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg
     1321 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
     1381 ctctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag gataacatgg
     1441 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1501 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1561 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1621 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1681 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1741 ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1801 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1861 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1921 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1981 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2041 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2101 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2161 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2221 cacactggct caccttcggg tgggcctttc tgcgtttata
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