##gff-version 3
##sequence-region BBa_K986013 1 2260
BBa_K986013	.	promoter	1	204	.	+	0	ID=BBa_K737026;Name=BBa_K737026
BBa_K986013	.	engineered_region	213	2260	.	+	0	ID=BBa_S05123;Name=BBa_S05123
BBa_K986013	.	engineered_region	213	1383	.	+	0	ID=BBa_S05104;Name=BBa_S05104;Parent=BBa_S05123
BBa_K986013	.	CDS	231	1358	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05104
BBa_K986013	.	engineered_tag	1359	1383	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986013	.	tag	1320	1358	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986013	.	engineered_region	231	1358	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K986013	.	CDS	231	1358	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986013	.	ribosome_entry_site	213	224	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05104
BBa_K986013	.	engineered_region	1392	2260	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05123
BBa_K986013	.	terminator	2132	2211	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986013	.	engineered_region	2132	2211	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986013	.	stem_loop	2143	2186	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986013	.	terminator	2220	2260	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986013	.	stem_loop	2227	2246	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986013	.	engineered_region	2220	2260	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986013	.	stop_codon	2253	2253	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986013	.	polyA_site	2247	2260	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986013	.	engineered_region	1410	2123	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986013	.	sequence_alteration	1761	1761	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986013	.	CDS	1410	2120	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986013	.	ribosome_entry_site	1392	1403	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986013	.	conserved_region	1396	1399	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K986013
atgaaacgatgagcaatctgtagagtttgattcagaccttctatattttcccgcttatccgtgccccatctcccatttt
ccctcacccacgccgtcaccgccttgtcatctttctgacaccttactatcttacaaatgtaacaaaaaagttatttttc
tgtaattcgagcatgtcatgttaccccgcgagcataaaacgcgtgatactagagaaagacatgagttactagatggtga
atgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagg
gcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacga
gttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggc
cccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgaca
tccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggt
gaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctcc
gacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctga
agggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaa
gaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatc
gtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata