LOCUS       BBa_K986014             2149 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737023+BBa_S05121
ACCESSION   BBa_K986014
VERSION     BBa_K986014.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..113
                     /label=RBS-3\Weak
     RBS             107..110
                     /label=RBS
     misc_feature    101..113
                     /label=BBa_B0032
     RBS             101..113
                     /label=BBa_B0032
     misc_feature    1209..1247
                     /label=LVA
     CDS             120..1247
                     /label=lacI-LVA
     misc_feature    120..1247
                     /label=BBa_C0012
     misc_feature    1248..1272
                     /label=Help:Barcodes
     CDS             120..1247
                     /label=BBa_C0012
     misc_feature    101..1272
                     /label=BBa_S05101
     misc_feature    1285..1288
                     /label=conserved
     RBS             1281..1292
                     /label=BBa_B0034
     CDS             1299..2009
                     /label=mCherry
     misc_feature    1650..1650
                     /label=C->T (removing PstI site)
     misc_feature    1299..2012
                     /label=BBa_J06504
     stem_loop       2032..2075
                     /label=stem_loop
     misc_feature    2021..2100
                     /label=BBa_B0010
     terminator      2021..2100
                     /label=BBa_B0010
     stem_loop       2116..2135
                     /label=T7 TE
     misc_feature    2142..2142
                     /label=stop
     polyA_site      2136..2149
                     /label=polya
     misc_feature    2109..2149
                     /label=BBa_B0012
     terminator      2109..2149
                     /label=BBa_B0012
     misc_feature    1281..2149
                     /label=BBa_J06702
     misc_feature    101..2149
                     /label=BBa_S05121
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga
      121 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
      181 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
      301 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
      361 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
      421 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
      481 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
      541 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
      601 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
      661 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
      721 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
      781 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
      841 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
      901 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
      961 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     1021 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     1081 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
     1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1201 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct
     1261 agtgtagatc tctactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
     1321 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1381 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1441 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1501 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1561 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1621 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
     1681 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1741 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1801 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1861 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1921 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1981 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     2041 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2101 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//