##gff-version 3
##sequence-region BBa_K986014 1 2149
BBa_K986014	.	engineered_region	101	2149	.	+	0	ID=BBa_S05121;Name=BBa_S05121
BBa_K986014	.	engineered_region	1281	2149	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05121
BBa_K986014	.	terminator	2021	2100	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986014	.	engineered_region	2021	2100	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986014	.	stem_loop	2032	2075	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986014	.	terminator	2109	2149	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986014	.	stem_loop	2116	2135	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986014	.	engineered_region	2109	2149	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986014	.	stop_codon	2142	2142	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986014	.	polyA_site	2136	2149	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986014	.	engineered_region	1299	2012	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986014	.	sequence_alteration	1650	1650	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986014	.	CDS	1299	2009	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986014	.	ribosome_entry_site	1281	1292	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986014	.	conserved_region	1285	1288	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986014	.	engineered_region	101	1272	.	+	0	ID=BBa_S05101;Name=BBa_S05101;Parent=BBa_S05121
BBa_K986014	.	ribosome_entry_site	101	113	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05101
BBa_K986014	.	ribosome_entry_site	101	113	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986014	.	ribosome_entry_site	107	110	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986014	.	engineered_region	101	113	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986014	.	CDS	120	1247	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05101
BBa_K986014	.	engineered_tag	1248	1272	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986014	.	tag	1209	1247	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986014	.	engineered_region	120	1247	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K986014	.	CDS	120	1247	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986014	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023
>BBa_K986014
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgca
gagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaa
aagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgct
gattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaa
ctgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcg
cgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa
tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcga
ctgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgc
gtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggag
tgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgat
cagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacg
acgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccag
cgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaaga
aaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggag
ttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcc
cctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccc
tcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgag
ggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacg
gcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatggg
ctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaag
gacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacg
tcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgcca
ctccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata