##gff-version 3
##sequence-region BBa_K986015 1 2148
BBa_K986015	.	engineered_region	101	2148	.	+	0	ID=BBa_S05123;Name=BBa_S05123
BBa_K986015	.	engineered_region	101	1271	.	+	0	ID=BBa_S05104;Name=BBa_S05104;Parent=BBa_S05123
BBa_K986015	.	CDS	119	1246	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05104
BBa_K986015	.	engineered_region	119	1246	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K986015	.	tag	1208	1246	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986015	.	engineered_tag	1247	1271	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986015	.	CDS	119	1246	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986015	.	ribosome_entry_site	101	112	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05104
BBa_K986015	.	engineered_region	1280	2148	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05123
BBa_K986015	.	ribosome_entry_site	1280	1291	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986015	.	conserved_region	1284	1287	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986015	.	terminator	2108	2148	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986015	.	stem_loop	2115	2134	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986015	.	polyA_site	2135	2148	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986015	.	stop_codon	2141	2141	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986015	.	engineered_region	2108	2148	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986015	.	engineered_region	1298	2011	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986015	.	sequence_alteration	1649	1649	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986015	.	CDS	1298	2008	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986015	.	terminator	2020	2099	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986015	.	engineered_region	2020	2099	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986015	.	stem_loop	2031	2074	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986015	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023
>BBa_K986015
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagaaagacatgagttactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagt
tcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgccc
ctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccct
cagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagg
gcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacgg
cgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggc
tgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaagg
acggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgt
caacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccac
tccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata