LOCUS       BBa_K986016             1680 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737023+BBa_S05124
ACCESSION   BBa_K986016
VERSION     BBa_K986016.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..112
                     /label=BBa_J61117
     CDS             122..738
                     /label=tetR
     misc_feature    739..772
                     /label=SsrA
     misc_feature    779..803
                     /label=Help:Barcodes
     CDS             119..803
                     /label=BBa_C0040
     misc_feature    101..803
                     /label=BBa_S05105
     misc_feature    816..819
                     /label=conserved
     RBS             812..823
                     /label=BBa_B0034
     CDS             830..1540
                     /label=mCherry
     misc_feature    1181..1181
                     /label=C->T (removing PstI site)
     misc_feature    830..1543
                     /label=BBa_J06504
     stem_loop       1563..1606
                     /label=stem_loop
     misc_feature    1552..1631
                     /label=BBa_B0010
     terminator      1552..1631
                     /label=BBa_B0010
     stem_loop       1647..1666
                     /label=T7 TE
     misc_feature    1673..1673
                     /label=stop
     polyA_site      1667..1680
                     /label=polya
     misc_feature    1640..1680
                     /label=BBa_B0012
     terminator      1640..1680
                     /label=BBa_B0012
     misc_feature    812..1680
                     /label=BBa_J06702
     misc_feature    101..1680
                     /label=BBa_S05124
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacatga gttactagat
      121 gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg
      181 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt
      241 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga
      301 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa
      361 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt
      421 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg
      481 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac
      541 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc
      601 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg
      661 tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact
      721 taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca
      781 ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga tggtgagcaa
      841 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      901 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      961 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     1021 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     1081 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1141 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat
     1201 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     1261 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     1321 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     1381 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     1441 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     1501 ccgccactcc accggcggca tggacgagct gtacaagtaa taatactaga gccaggcatc
     1561 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1621 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//