##gff-version 3
##sequence-region BBa_K986016 1 1680
BBa_K986016	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023
BBa_K986016	.	engineered_region	101	1680	.	+	0	ID=BBa_S05124;Name=BBa_S05124
BBa_K986016	.	engineered_region	812	1680	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05124
BBa_K986016	.	terminator	1640	1680	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986016	.	stop_codon	1673	1673	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986016	.	polyA_site	1667	1680	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986016	.	stem_loop	1647	1666	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986016	.	engineered_region	1640	1680	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986016	.	terminator	1552	1631	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986016	.	engineered_region	1552	1631	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986016	.	stem_loop	1563	1606	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986016	.	ribosome_entry_site	812	823	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986016	.	conserved_region	816	819	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986016	.	engineered_region	830	1543	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986016	.	sequence_alteration	1181	1181	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986016	.	CDS	830	1540	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986016	.	engineered_region	101	803	.	+	0	ID=BBa_S05105;Name=BBa_S05105;Parent=BBa_S05124
BBa_K986016	.	ribosome_entry_site	101	112	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05105
BBa_K986016	.	CDS	119	803	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05105
BBa_K986016	.	engineered_tag	779	803	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K986016	.	CDS	122	738	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K986016	.	tag	739	772	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K986016
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagaaagacatgagttactagatgtccagattagataaaagtaaagtgattaacagcgcat
tagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctac
attgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcac
ttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtc
atcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctt
tttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgta
ttggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgac
aagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattaga
aaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgc
tagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatca
aggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgaggg
ccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctg
tcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttcc
ccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgca
ggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagacc
atgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagc
tgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgccta
caacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggc
cgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata