LOCUS       BBa_K986017             2261 bp    DNA     linear       01-Jul-2024
DEFINITION  BBa_K737026+BBa_S05121
ACCESSION   BBa_K986017
VERSION     BBa_K986017.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..225
                     /label=RBS-3\Weak
     RBS             219..222
                     /label=RBS
     misc_feature    213..225
                     /label=BBa_B0032
     RBS             213..225
                     /label=BBa_B0032
     misc_feature    1321..1359
                     /label=LVA
     CDS             232..1359
                     /label=lacI-LVA
     misc_feature    232..1359
                     /label=BBa_C0012
     misc_feature    1360..1384
                     /label=Help:Barcodes
     CDS             232..1359
                     /label=BBa_C0012
     misc_feature    213..1384
                     /label=BBa_S05101
     misc_feature    1397..1400
                     /label=conserved
     RBS             1393..1404
                     /label=BBa_B0034
     CDS             1411..2121
                     /label=mCherry
     misc_feature    1762..1762
                     /label=C->T (removing PstI site)
     misc_feature    1411..2124
                     /label=BBa_J06504
     stem_loop       2144..2187
                     /label=stem_loop
     misc_feature    2133..2212
                     /label=BBa_B0010
     terminator      2133..2212
                     /label=BBa_B0010
     stem_loop       2228..2247
                     /label=T7 TE
     misc_feature    2254..2254
                     /label=stop
     polyA_site      2248..2261
                     /label=polya
     misc_feature    2221..2261
                     /label=BBa_B0012
     terminator      2221..2261
                     /label=BBa_B0012
     misc_feature    1393..2261
                     /label=BBa_J06702
     misc_feature    213..2261
                     /label=BBa_S05121
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agtcacacag gaaagtacta gatggtgaat
      241 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
      301 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      361 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      421 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      481 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      541 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      601 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      661 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      721 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      781 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      841 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      901 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      961 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     1021 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     1081 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     1141 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     1201 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1261 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1321 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
     1381 tctctactag agaaagagga gaaatactag atggtgagca agggcgagga ggataacatg
     1441 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     1501 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1561 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     1621 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     1681 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     1741 accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc
     1801 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1861 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     1921 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     1981 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     2041 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     2101 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
     2161 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2221 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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