##gff-version 3
##sequence-region BBa_K986017 1 2261
BBa_K986017	.	promoter	1	204	.	+	0	ID=BBa_K737026;Name=BBa_K737026
BBa_K986017	.	engineered_region	213	2261	.	+	0	ID=BBa_S05121;Name=BBa_S05121
BBa_K986017	.	engineered_region	1393	2261	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05121
BBa_K986017	.	ribosome_entry_site	1393	1404	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986017	.	conserved_region	1397	1400	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986017	.	terminator	2221	2261	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986017	.	stem_loop	2228	2247	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986017	.	stop_codon	2254	2254	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986017	.	engineered_region	2221	2261	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986017	.	polyA_site	2248	2261	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986017	.	engineered_region	1411	2124	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986017	.	CDS	1411	2121	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986017	.	sequence_alteration	1762	1762	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986017	.	terminator	2133	2212	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986017	.	engineered_region	2133	2212	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986017	.	stem_loop	2144	2187	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986017	.	engineered_region	213	1384	.	+	0	ID=BBa_S05101;Name=BBa_S05101;Parent=BBa_S05121
BBa_K986017	.	ribosome_entry_site	213	225	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05101
BBa_K986017	.	ribosome_entry_site	219	222	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986017	.	ribosome_entry_site	213	225	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986017	.	engineered_region	213	225	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986017	.	CDS	232	1359	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05101
BBa_K986017	.	engineered_tag	1360	1384	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986017	.	CDS	232	1359	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986017	.	tag	1321	1359	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986017	.	engineered_region	232	1359	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K986017
atgaaacgatgagcaatctgtagagtttgattcagaccttctatattttcccgcttatccgtgccccatctcccatttt
ccctcacccacgccgtcaccgccttgtcatctttctgacaccttactatcttacaaatgtaacaaaaaagttatttttc
tgtaattcgagcatgtcatgttaccccgcgagcataaaacgcgtgatactagagtcacacaggaaagtactagatggtg
aatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacc
aggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgt
ggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaa
attgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcg
aagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgacca
ggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaac
agtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgc
tgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaat
tcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggc
atcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgc
gcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccat
caaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggc
aatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgc
tttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaag
ggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacg
agttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtgg
ccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgac
atccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtgg
tgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctc
cgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctg
aagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggcca
agaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccat
cgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata