LOCUS       BBa_K986019             2148 bp    DNA     linear       22-Nov-2024
DEFINITION  BBa_K737023+BBa_S05125
ACCESSION   BBa_K986019
VERSION     BBa_K986019.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..112
                     /label=BBa_J61101
     misc_feature    1208..1246
                     /label=LVA
     CDS             119..1246
                     /label=lacI-LVA
     misc_feature    119..1246
                     /label=BBa_C0012
     misc_feature    1247..1271
                     /label=Help:Barcodes
     CDS             119..1246
                     /label=BBa_C0012
     misc_feature    101..1271
                     /label=BBa_S05107
     misc_feature    1284..1287
                     /label=conserved
     RBS             1280..1291
                     /label=BBa_B0034
     CDS             1298..2008
                     /label=mCherry
     misc_feature    1649..1649
                     /label=C->T (removing PstI site)
     misc_feature    1298..2011
                     /label=BBa_J06504
     stem_loop       2031..2074
                     /label=stem_loop
     misc_feature    2020..2099
                     /label=BBa_B0010
     terminator      2020..2099
                     /label=BBa_B0010
     stem_loop       2115..2134
                     /label=T7 TE
     misc_feature    2141..2141
                     /label=stop
     polyA_site      2135..2148
                     /label=polya
     misc_feature    2108..2148
                     /label=BBa_B0012
     terminator      2108..2148
                     /label=BBa_B0012
     misc_feature    1280..2148
                     /label=BBa_J06702
     misc_feature    101..2148
                     /label=BBa_S05125
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacagga cctactagat
      121 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
      181 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      241 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      301 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
      361 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
      421 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
      481 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
      541 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      601 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      661 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      721 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      781 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
      841 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
      901 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
      961 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     1021 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     1081 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1201 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta
     1261 gtgtagatct ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga
     1321 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1381 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1441 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1501 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1561 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1621 cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa
     1681 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1741 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1801 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1861 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1921 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1981 cggcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa
     2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//