LOCUS       BBa_K986020             1681 bp    DNA     linear       25-Nov-2024
DEFINITION  BBa_K737023+BBa_S05122
ACCESSION   BBa_K986020
VERSION     BBa_K986020.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=BBa_K737023
     RBS             101..113
                     /label=RBS-3\Weak
     RBS             107..110
                     /label=RBS
     misc_feature    101..113
                     /label=BBa_B0032
     RBS             101..113
                     /label=BBa_B0032
     CDS             123..739
                     /label=tetR
     misc_feature    740..773
                     /label=SsrA
     misc_feature    780..804
                     /label=Help:Barcodes
     CDS             120..804
                     /label=BBa_C0040
     misc_feature    101..804
                     /label=BBa_S05102
     misc_feature    817..820
                     /label=conserved
     RBS             813..824
                     /label=BBa_B0034
     CDS             831..1541
                     /label=mCherry
     misc_feature    1182..1182
                     /label=C->T (removing PstI site)
     misc_feature    831..1544
                     /label=BBa_J06504
     stem_loop       1564..1607
                     /label=stem_loop
     misc_feature    1553..1632
                     /label=BBa_B0010
     terminator      1553..1632
                     /label=BBa_B0010
     stem_loop       1648..1667
                     /label=T7 TE
     misc_feature    1674..1674
                     /label=stop
     polyA_site      1668..1681
                     /label=polya
     misc_feature    1641..1681
                     /label=BBa_B0012
     terminator      1641..1681
                     /label=BBa_B0012
     misc_feature    813..1681
                     /label=BBa_J06702
     misc_feature    101..1681
                     /label=BBa_S05122
ORIGIN
        1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct
       61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga
      121 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      181 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      241 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      301 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      361 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
      421 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
      481 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
      541 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
      601 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
      661 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
      721 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
      781 actgatagtg ctagtgtaga tcactactag agaaagagga gaaatactag atggtgagca
      841 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      901 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      961 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1021 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1081 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1141 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cttgcaggac ggcgagttca
     1201 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1261 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1321 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1381 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1441 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1501 gccgccactc caccggcggc atggacgagc tgtacaagta ataatactag agccaggcat
     1561 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1621 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1681 a
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