##gff-version 3
##sequence-region BBa_K986020 1 1681
BBa_K986020	.	engineered_region	101	1681	.	+	0	ID=BBa_S05122;Name=BBa_S05122
BBa_K986020	.	engineered_region	813	1681	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05122
BBa_K986020	.	engineered_region	831	1544	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986020	.	CDS	831	1541	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986020	.	sequence_alteration	1182	1182	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986020	.	terminator	1553	1632	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986020	.	engineered_region	1553	1632	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986020	.	stem_loop	1564	1607	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986020	.	ribosome_entry_site	813	824	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986020	.	conserved_region	817	820	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986020	.	terminator	1641	1681	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986020	.	engineered_region	1641	1681	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986020	.	stem_loop	1648	1667	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986020	.	polyA_site	1668	1681	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986020	.	stop_codon	1674	1674	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986020	.	engineered_region	101	804	.	+	0	ID=BBa_S05102;Name=BBa_S05102;Parent=BBa_S05122
BBa_K986020	.	ribosome_entry_site	101	113	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05102
BBa_K986020	.	ribosome_entry_site	101	113	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986020	.	engineered_region	101	113	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986020	.	ribosome_entry_site	107	110	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986020	.	CDS	120	804	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05102
BBa_K986020	.	CDS	123	739	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K986020	.	engineered_tag	780	804	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K986020	.	tag	740	773	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K986020	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023
>BBa_K986020
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgca
ttagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagccta
cattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactca
cttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagt
catcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcct
ttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgt
attggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacga
caagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattag
aaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtg
ctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcct
acaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgaggg
ccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata