LOCUS       BBa_K986021             1793 bp    DNA     linear       05-Nov-2024
DEFINITION  BBa_K737026+BBa_S05122
ACCESSION   BBa_K986021
VERSION     BBa_K986021.1  
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label=BBa_K737026
     RBS             213..225
                     /label=RBS-3\Weak
     RBS             219..222
                     /label=RBS
     misc_feature    213..225
                     /label=BBa_B0032
     RBS             213..225
                     /label=BBa_B0032
     CDS             235..851
                     /label=tetR
     misc_feature    852..885
                     /label=SsrA
     misc_feature    892..916
                     /label=Help:Barcodes
     CDS             232..916
                     /label=BBa_C0040
     misc_feature    213..916
                     /label=BBa_S05102
     misc_feature    929..932
                     /label=conserved
     RBS             925..936
                     /label=BBa_B0034
     CDS             943..1653
                     /label=mCherry
     misc_feature    1294..1294
                     /label=C->T (removing PstI site)
     misc_feature    943..1656
                     /label=BBa_J06504
     stem_loop       1676..1719
                     /label=stem_loop
     misc_feature    1665..1744
                     /label=BBa_B0010
     terminator      1665..1744
                     /label=BBa_B0010
     stem_loop       1760..1779
                     /label=T7 TE
     misc_feature    1786..1786
                     /label=stop
     polyA_site      1780..1793
                     /label=polya
     misc_feature    1753..1793
                     /label=BBa_B0012
     terminator      1753..1793
                     /label=BBa_B0012
     misc_feature    925..1793
                     /label=BBa_J06702
     misc_feature    213..1793
                     /label=BBa_S05122
ORIGIN
        1 atgaaacgat gagcaatctg tagagtttga ttcagacctt ctatattttc ccgcttatcc
       61 gtgccccatc tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac
      121 cttactatct tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt
      181 accccgcgag cataaaacgc gtgatactag agtcacacag gaaagtacta gatgtccaga
      241 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
      301 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
      361 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
      421 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
      481 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
      541 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
      601 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
      661 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
      721 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
      781 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
      841 gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag
      901 tgctagtgta gatcactact agagaaagag gagaaatact agatggtgag caagggcgag
      961 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1021 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1081 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1141 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1201 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1261 ggcggcgtgg tgaccgtgac ccaggactcc tccttgcagg acggcgagtt catctacaag
     1321 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1381 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1441 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1501 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1561 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1621 tccaccggcg gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa
     1681 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1741 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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