##gff-version 3
##sequence-region BBa_K986021 1 1793
BBa_K986021	.	engineered_region	213	1793	.	+	0	ID=BBa_S05122;Name=BBa_S05122
BBa_K986021	.	engineered_region	925	1793	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05122
BBa_K986021	.	terminator	1753	1793	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986021	.	polyA_site	1780	1793	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986021	.	stem_loop	1760	1779	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986021	.	stop_codon	1786	1786	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986021	.	engineered_region	1753	1793	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986021	.	engineered_region	943	1656	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986021	.	CDS	943	1653	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986021	.	sequence_alteration	1294	1294	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986021	.	terminator	1665	1744	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986021	.	stem_loop	1676	1719	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986021	.	engineered_region	1665	1744	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986021	.	ribosome_entry_site	925	936	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986021	.	conserved_region	929	932	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986021	.	engineered_region	213	916	.	+	0	ID=BBa_S05102;Name=BBa_S05102;Parent=BBa_S05122
BBa_K986021	.	ribosome_entry_site	213	225	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05102
BBa_K986021	.	engineered_region	213	225	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986021	.	ribosome_entry_site	219	222	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986021	.	ribosome_entry_site	213	225	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986021	.	CDS	232	916	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05102
BBa_K986021	.	engineered_tag	892	916	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K986021	.	CDS	235	851	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K986021	.	tag	852	885	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K986021	.	promoter	1	204	.	+	0	ID=BBa_K737026;Name=BBa_K737026
>BBa_K986021
atgaaacgatgagcaatctgtagagtttgattcagaccttctatattttcccgcttatccgtgccccatctcccatttt
ccctcacccacgccgtcaccgccttgtcatctttctgacaccttactatcttacaaatgtaacaaaaaagttatttttc
tgtaattcgagcatgtcatgttaccccgcgagcataaaacgcgtgatactagagtcacacaggaaagtactagatgtcc
agattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgta
aactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcctt
agccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaat
aacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaa
aacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcact
cagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaaca
cctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttct
tattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaa
ctacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtg
agcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacg
gccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaa
gggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacccc
gccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcg
gcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaactt
cccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggc
gccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctaca
aggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggacta
caccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata