LOCUS       BBa_K986025             3020 bp    DNA     linear       26-Nov-2024
DEFINITION  BBa_S05138+K986001
ACCESSION   BBa_K986025
VERSION     BBa_K986025.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..739
                     /label=GFP
     misc_feature    740..772
                     /label=LVA
     CDS             20..775
                     /label=BBa_K082003
     misc_feature    1..775
                     /label=BBa_S05100
     stem_loop       795..838
                     /label=stem_loop
     misc_feature    784..863
                     /label=BBa_B0010
     terminator      784..863
                     /label=BBa_B0010
     misc_feature    1..863
                     /label=BBa_K986001
     misc_feature    874..890
                     /label=lac O1
     promoter        891..896
                     /label=-35
     misc_feature    897..913
                     /label=lac O1
     promoter        914..919
                     /label=-10
     misc_feature    872..925
                     /label=BBa_R0011
     promoter        872..925
                     /label=BBa_R0011
     RBS             935..947
                     /label=RBS-3\Weak
     RBS             941..944
                     /label=RBS
     misc_feature    935..947
                     /label=BBa_B0032
     RBS             935..947
                     /label=BBa_B0032
     CDS             954..3020
                     /label=BBa_K986000
     misc_feature    935..3020
                     /label=BBa_S05136
     misc_feature    872..3020
                     /label=BBa_S05138
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaatacta
      781 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      841 gttgtttgtc ggtgaacgct ctctactaga gaattgtgag cggataacaa ttgacattgt
      901 gagcggataa caagatactg agcacatact agagtcacac aggaaagtac tagatgggtc
      961 aggaaaagct atacatcgaa aaagagctca gttggttatc gttcaatgaa cgcgtgcttc
     1021 aggaagcggc ggacaaatct aacccgctga ttgaaaggat gcgtttcctg gggatctatt
     1081 ccaataacct tgatgagttc tataaagtcc gcttcgctga actgaagcga cgcatcatta
     1141 ttagcgaaga acaaggctcc aactctcatt cccgccattt actgggcaaa attcagtccc
     1201 gggtgctgaa agccgatcag gagtttgacg gcctctacaa cgagctattg ctggagatgg
     1261 cgcgcaacca gatcttcctg attaatgaac gccagctctc cgtcaatcaa caaaactggc
     1321 tgcgtcatta ttttaagcag tatctgcgtc agcacattac gccgatttta atcaatcctg
     1381 acactgactt agtgcagttc ctgaaagatg attacaccta tctggcggtg gaaattatcc
     1441 gtggcgatac catccgttac gcgctgctgg agatcccatc agataaagtg ccgcgctttg
     1501 tgaatttacc gccagaagcg ccgcgtcgac gcaagccgat gattcttctg gataacattc
     1561 tgcgttactg ccttgatgat attttcaaag gcttctttga ttatgacgcg ctgaatgcct
     1621 attcaatgaa gatgacccgc gatgccgaat acgatttagt gcatgagatg gaagccagcc
     1681 tgatggagtt gatgtcttcc agtctcaagc agcgtttaac tgctgagccg gtgcgttttg
     1741 tttatcagcg cgatatgccc aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt
     1801 cccgctacga ctccatcgtc cccggcggtc gttatcataa ttttaaagac tttattaatt
     1861 tccccaatgt cggcaaagcc aatctggtga acaaaccact gccgcgttta cgccatattt
     1921 ggtttgataa agcccagttc cgcaatggtt ttgatgccat tcgcgaacgc gatgtgttgc
     1981 tctattatcc ttatcacacc tttgagcatg tgctggaact gctgcgtcag gcttcgttcg
     2041 acccgagcgt actggcgatt aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca
     2101 tcgactcgat gatccacgcc gcacataacg gtaagaaagt caccgtggtg gttgagttac
     2161 aggcgcgttt cgacgaagaa gccaacattc actgggcgaa gcgcctgacc gaagcaggcg
     2221 tgcacgttat cttctctgcg ccggggctga aaattcacgc caaactgttc ctgatttcac
     2281 gtaaagaaaa cggtgaagtg gtgcgttatg cacacatcgg gaccgggaac tttaacgaaa
     2341 aaaccgcgcg tctttatact gactattcgt tgctgaccgc cgatgcgcgc atcaccaacg
     2401 aagtacggcg ggtatttaac tttattgaaa acccataccg tccggtgaca tttgattatt
     2461 taatggtatc gccgcaaaac tcccgccgcc tattgtatga aatggtggac cgcgagatcg
     2521 ccaacgcgca gcaagggctg cccagtggta tcaccctgaa gctaaataac cttgtcgata
     2581 aaggcctggt tgatcgtctg tatgcggcct ccagctccgg cgtaccggtt aatctgctgg
     2641 ttcgcggaat gtgttcgctg atccccaatc tggaaggcat tagcgacaac attcgtgcca
     2701 tcagtattgt tgaccgttac cttgaacatg accgggttta tatttttgaa aatggcggcg
     2761 ataaaaaggt ctacctttct tccgccgact ggatgacgcg caatattgat tatcgtattg
     2821 aagtggcgac gccgctgctc gatccgcgcc tgaagcagcg ggtactggac atcatcgaca
     2881 tattgttcag cgatacggtc aaagcacgtt atatcgataa agaactcagt aatcgctacg
     2941 ttccccgcgg caatcgccgc aaagtacggg cgcagttggc gatttatgac tacatcaaat
     3001 cactcgaaca acctgaataa
//