LOCUS       BBa_K986028             3020 bp    DNA     linear       18-Jun-2024
DEFINITION  BBa_R0011+BBa_S05140
ACCESSION   BBa_K986028
VERSION     BBa_K986028.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..2149
                     /label=BBa_K986000
     RBS             2158..2170
                     /label=RBS-3\Weak
     RBS             2164..2167
                     /label=RBS
     misc_feature    2158..2170
                     /label=BBa_B0032
     RBS             2158..2170
                     /label=BBa_B0032
     misc_feature    2177..2896
                     /label=GFP
     misc_feature    2897..2929
                     /label=LVA
     CDS             2177..2932
                     /label=BBa_K082003
     misc_feature    2158..2932
                     /label=BBa_S05100
     stem_loop       2952..2995
                     /label=stem_loop
     misc_feature    2941..3020
                     /label=BBa_B0010
     terminator      2941..3020
                     /label=BBa_B0010
     misc_feature    2158..3020
                     /label=BBa_K986001
     misc_feature    83..3020
                     /label=BBa_S05137
     misc_feature    64..3020
                     /label=BBa_S05140
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgggtca ggaaaagcta tacatcgaaa aagagctcag
      121 ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg gacaaatcta acccgctgat
      181 tgaaaggatg cgtttcctgg ggatctattc caataacctt gatgagttct ataaagtccg
      241 cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa caaggctcca actctcattc
      301 ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa gccgatcagg agtttgacgg
      361 cctctacaac gagctattgc tggagatggc gcgcaaccag atcttcctga ttaatgaacg
      421 ccagctctcc gtcaatcaac aaaactggct gcgtcattat tttaagcagt atctgcgtca
      481 gcacattacg ccgattttaa tcaatcctga cactgactta gtgcagttcc tgaaagatga
      541 ttacacctat ctggcggtgg aaattatccg tggcgatacc atccgttacg cgctgctgga
      601 gatcccatca gataaagtgc cgcgctttgt gaatttaccg ccagaagcgc cgcgtcgacg
      661 caagccgatg attcttctgg ataacattct gcgttactgc cttgatgata ttttcaaagg
      721 cttctttgat tatgacgcgc tgaatgccta ttcaatgaag atgacccgcg atgccgaata
      781 cgatttagtg catgagatgg aagccagcct gatggagttg atgtcttcca gtctcaagca
      841 gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc gatatgccca atgcgctggt
      901 tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac tccatcgtcc ccggcggtcg
      961 ttatcataat tttaaagact ttattaattt ccccaatgtc ggcaaagcca atctggtgaa
     1021 caaaccactg ccgcgtttac gccatatttg gtttgataaa gcccagttcc gcaatggttt
     1081 tgatgccatt cgcgaacgcg atgtgttgct ctattatcct tatcacacct ttgagcatgt
     1141 gctggaactg ctgcgtcagg cttcgttcga cccgagcgta ctggcgatta aaattaacat
     1201 ttaccgcgtg gcgaaagatt cacgcatcat cgactcgatg atccacgccg cacataacgg
     1261 taagaaagtc accgtggtgg ttgagttaca ggcgcgtttc gacgaagaag ccaacattca
     1321 ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc ttctctgcgc cggggctgaa
     1381 aattcacgcc aaactgttcc tgatttcacg taaagaaaac ggtgaagtgg tgcgttatgc
     1441 acacatcggg accgggaact ttaacgaaaa aaccgcgcgt ctttatactg actattcgtt
     1501 gctgaccgcc gatgcgcgca tcaccaacga agtacggcgg gtatttaact ttattgaaaa
     1561 cccataccgt ccggtgacat ttgattattt aatggtatcg ccgcaaaact cccgccgcct
     1621 attgtatgaa atggtggacc gcgagatcgc caacgcgcag caagggctgc ccagtggtat
     1681 caccctgaag ctaaataacc ttgtcgataa aggcctggtt gatcgtctgt atgcggcctc
     1741 cagctccggc gtaccggtta atctgctggt tcgcggaatg tgttcgctga tccccaatct
     1801 ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt gaccgttacc ttgaacatga
     1861 ccgggtttat atttttgaaa atggcggcga taaaaaggtc tacctttctt ccgccgactg
     1921 gatgacgcgc aatattgatt atcgtattga agtggcgacg ccgctgctcg atccgcgcct
     1981 gaagcagcgg gtactggaca tcatcgacat attgttcagc gatacggtca aagcacgtta
     2041 tatcgataaa gaactcagta atcgctacgt tccccgcggc aatcgccgca aagtacgggc
     2101 gcagttggcg atttatgact acatcaaatc actcgaacaa cctgaataat actagagtca
     2161 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     2221 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     2281 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     2341 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     2401 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     2461 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     2521 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     2581 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     2641 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     2701 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     2761 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2821 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2881 actatacaaa aggcctgctg caaacgacga aaactacgct ttagtagctt aatactagag
     2941 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3001 gtttgtcggt gaacgctctc
//