LOCUS       BBa_K986029             3019 bp    DNA     linear       29-Jun-2024
DEFINITION  BBa_R0040+BBa_S05140
ACCESSION   BBa_K986029
VERSION     BBa_K986029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..2148
                     /label=BBa_K986000
     RBS             2157..2169
                     /label=RBS-3\Weak
     RBS             2163..2166
                     /label=RBS
     misc_feature    2157..2169
                     /label=BBa_B0032
     RBS             2157..2169
                     /label=BBa_B0032
     misc_feature    2176..2895
                     /label=GFP
     misc_feature    2896..2928
                     /label=LVA
     CDS             2176..2931
                     /label=BBa_K082003
     misc_feature    2157..2931
                     /label=BBa_S05100
     stem_loop       2951..2994
                     /label=stem_loop
     misc_feature    2940..3019
                     /label=BBa_B0010
     terminator      2940..3019
                     /label=BBa_B0010
     misc_feature    2157..3019
                     /label=BBa_K986001
     misc_feature    82..3019
                     /label=BBa_S05137
     misc_feature    63..3019
                     /label=BBa_S05140
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgggtcag gaaaagctat acatcgaaaa agagctcagt
      121 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
      181 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
      241 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
      301 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga gtttgacggc
      361 ctctacaacg agctattgct ggagatggcg cgcaaccaga tcttcctgat taatgaacgc
      421 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
      481 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
      541 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
      601 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc
      661 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
      721 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
      781 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
      841 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
      901 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
      961 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     1021 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     1081 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     1141 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     1201 taccgcgtgg cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt
     1261 aagaaagtca ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     1321 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     1381 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt gcgttatgca
     1441 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     1501 ctgaccgccg atgcgcgcat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     1561 ccataccgtc cggtgacatt tgattattta atggtatcgc cgcaaaactc ccgccgccta
     1621 ttgtatgaaa tggtggaccg cgagatcgcc aacgcgcagc aagggctgcc cagtggtatc
     1681 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
     1741 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
     1801 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
     1861 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
     1921 atgacgcgca atattgatta tcgtattgaa gtggcgacgc cgctgctcga tccgcgcctg
     1981 aagcagcggg tactggacat catcgacata ttgttcagcg atacggtcaa agcacgttat
     2041 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
     2101 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataata ctagagtcac
     2161 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
     2221 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     2281 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     2341 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     2401 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     2461 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     2521 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     2581 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     2641 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     2701 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2761 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     2821 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2881 ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta atactagagc
     2941 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3001 tttgtcggtg aacgctctc
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