##gff-version 3
##sequence-region BBa_K986029 1 3019
BBa_K986029	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K986029	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K986029	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K986029	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K986029	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K986029	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K986029	.	engineered_region	63	3019	.	+	0	ID=BBa_S05140;Name=BBa_S05140
BBa_K986029	.	engineered_region	82	3019	.	+	0	ID=BBa_S05137;Name=BBa_S05137;Parent=BBa_S05140
BBa_K986029	.	CDS	82	2148	.	+	0	ID=BBa_K986000;Name=BBa_K986000;Parent=BBa_S05137
BBa_K986029	.	sequence_feature	2157	3019	.	+	0	ID=BBa_K986001;Name=BBa_K986001;Parent=BBa_S05137
BBa_K986029	.	terminator	2940	3019	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K986001
BBa_K986029	.	engineered_region	2940	3019	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986029	.	stem_loop	2951	2994	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986029	.	engineered_region	2157	2931	.	+	0	ID=BBa_S05100;Name=BBa_S05100;Parent=BBa_K986001
BBa_K986029	.	CDS	2176	2931	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_S05100
BBa_K986029	.	engineered_region	2176	2895	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K986029	.	tag	2896	2928	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K986029	.	ribosome_entry_site	2157	2169	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05100
BBa_K986029	.	ribosome_entry_site	2157	2169	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986029	.	engineered_region	2157	2169	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986029	.	ribosome_entry_site	2163	2166	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986029	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05140
BBa_K986029	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986029	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986029	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K986029
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgggtcaggaaaagctatacatcgaaaaagagctcagttggttatcgttcaatgaacgcgtgcttcaggaagcggc
ggacaaatctaacccgctgattgaaaggatgcgtttcctggggatctattccaataaccttgatgagttctataaagtc
cgcttcgctgaactgaagcgacgcatcattattagcgaagaacaaggctccaactctcattcccgccatttactgggca
aaattcagtcccgggtgctgaaagccgatcaggagtttgacggcctctacaacgagctattgctggagatggcgcgcaa
ccagatcttcctgattaatgaacgccagctctccgtcaatcaacaaaactggctgcgtcattattttaagcagtatctg
cgtcagcacattacgccgattttaatcaatcctgacactgacttagtgcagttcctgaaagatgattacacctatctgg
cggtggaaattatccgtggcgataccatccgttacgcgctgctggagatcccatcagataaagtgccgcgctttgtgaa
tttaccgccagaagcgccgcgtcgacgcaagccgatgattcttctggataacattctgcgttactgccttgatgatatt
ttcaaaggcttctttgattatgacgcgctgaatgcctattcaatgaagatgacccgcgatgccgaatacgatttagtgc
atgagatggaagccagcctgatggagttgatgtcttccagtctcaagcagcgtttaactgctgagccggtgcgttttgt
ttatcagcgcgatatgcccaatgcgctggttgaagtgttacgcgaaaaactgactatttcccgctacgactccatcgtc
cccggcggtcgttatcataattttaaagactttattaatttccccaatgtcggcaaagccaatctggtgaacaaaccac
tgccgcgtttacgccatatttggtttgataaagcccagttccgcaatggttttgatgccattcgcgaacgcgatgtgtt
gctctattatccttatcacacctttgagcatgtgctggaactgctgcgtcaggcttcgttcgacccgagcgtactggcg
attaaaattaacatttaccgcgtggcgaaagattcacgcatcatcgactcgatgatccacgccgcacataacggtaaga
aagtcaccgtggtggttgagttacaggcgcgtttcgacgaagaagccaacattcactgggcgaagcgcctgaccgaagc
aggcgtgcacgttatcttctctgcgccggggctgaaaattcacgccaaactgttcctgatttcacgtaaagaaaacggt
gaagtggtgcgttatgcacacatcgggaccgggaactttaacgaaaaaaccgcgcgtctttatactgactattcgttgc
tgaccgccgatgcgcgcatcaccaacgaagtacggcgggtatttaactttattgaaaacccataccgtccggtgacatt
tgattatttaatggtatcgccgcaaaactcccgccgcctattgtatgaaatggtggaccgcgagatcgccaacgcgcag
caagggctgcccagtggtatcaccctgaagctaaataaccttgtcgataaaggcctggttgatcgtctgtatgcggcct
ccagctccggcgtaccggttaatctgctggttcgcggaatgtgttcgctgatccccaatctggaaggcattagcgacaa
cattcgtgccatcagtattgttgaccgttaccttgaacatgaccgggtttatatttttgaaaatggcggcgataaaaag
gtctacctttcttccgccgactggatgacgcgcaatattgattatcgtattgaagtggcgacgccgctgctcgatccgc
gcctgaagcagcgggtactggacatcatcgacatattgttcagcgatacggtcaaagcacgttatatcgataaagaact
cagtaatcgctacgttccccgcggcaatcgccgcaaagtacgggcgcagttggcgatttatgactacatcaaatcactc
gaacaacctgaataatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcc
caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata
cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt
tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt
atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac
ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac
tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca
ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt
gtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttag
tagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctc