LOCUS BBa_K986030 5177 bp DNA linear 23-Nov-2024 DEFINITION BBa_K986014+BBa_K986028 ACCESSION BBa_K986030 VERSION BBa_K986030.1 FEATURES Location/Qualifiers promoter 1..92 /label=BBa_K737023 RBS 101..113 /label=RBS-3\Weak RBS 107..110 /label=RBS misc_feature 101..113 /label=BBa_B0032 RBS 101..113 /label=BBa_B0032 misc_feature 1209..1247 /label=LVA CDS 120..1247 /label=lacI-LVA misc_feature 120..1247 /label=BBa_C0012 misc_feature 1248..1272 /label=Help:Barcodes CDS 120..1247 /label=BBa_C0012 misc_feature 101..1272 /label=BBa_S05101 misc_feature 1285..1288 /label=conserved RBS 1281..1292 /label=BBa_B0034 CDS 1299..2009 /label=mCherry misc_feature 1650..1650 /label=C->T (removing PstI site) misc_feature 1299..2012 /label=BBa_J06504 stem_loop 2032..2075 /label=stem_loop misc_feature 2021..2100 /label=BBa_B0010 terminator 2021..2100 /label=BBa_B0010 stem_loop 2116..2135 /label=T7 TE misc_feature 2142..2142 /label=stop polyA_site 2136..2149 /label=polya misc_feature 2109..2149 /label=BBa_B0012 terminator 2109..2149 /label=BBa_B0012 misc_feature 1281..2149 /label=BBa_J06702 misc_feature 101..2149 /label=BBa_S05121 misc_feature 1..2149 /label=BBa_K986014 misc_feature 2160..2176 /label=lac O1 promoter 2177..2182 /label=-35 misc_feature 2183..2199 /label=lac O1 promoter 2200..2205 /label=-10 misc_feature 2158..2211 /label=BBa_R0011 promoter 2158..2211 /label=BBa_R0011 RBS 2221..2233 /label=RBS-3\Weak RBS 2227..2230 /label=RBS misc_feature 2221..2233 /label=BBa_B0032 RBS 2221..2233 /label=BBa_B0032 CDS 2240..4306 /label=BBa_K986000 RBS 4315..4327 /label=RBS-3\Weak RBS 4321..4324 /label=RBS misc_feature 4315..4327 /label=BBa_B0032 RBS 4315..4327 /label=BBa_B0032 misc_feature 4334..5053 /label=GFP misc_feature 5054..5086 /label=LVA CDS 4334..5089 /label=BBa_K082003 misc_feature 4315..5089 /label=BBa_S05100 stem_loop 5109..5152 /label=stem_loop misc_feature 5098..5177 /label=BBa_B0010 terminator 5098..5177 /label=BBa_B0010 misc_feature 4315..5177 /label=BBa_K986001 misc_feature 2240..5177 /label=BBa_S05137 misc_feature 2221..5177 /label=BBa_S05140 misc_feature 2158..5177 /label=BBa_K986028 ORIGIN 1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct 61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga 121 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc 181 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag 241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg 301 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc 361 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga 421 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac 481 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag 541 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca 601 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg 661 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc 721 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag 781 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg 841 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta 901 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag 961 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc 1021 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc 1081 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct 1141 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 1201 gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct 1261 agtgtagatc tctactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 1321 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 1381 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 1441 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 1501 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 1561 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 1621 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 1681 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 1741 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 1801 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 1861 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 1921 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 1981 ccggcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 2041 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2101 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagaat 2161 tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca catactagag 2221 tcacacagga aagtactaga tgggtcagga aaagctatac atcgaaaaag agctcagttg 2281 gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc cgctgattga 2341 aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata aagtccgctt 2401 cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaact ctcattcccg 2461 ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggagt ttgacggcct 2521 ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta atgaacgcca 2581 gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc tgcgtcagca 2641 cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga aagatgatta 2701 cacctatctg gcggtggaaa ttatccgtgg cgataccatc cgttacgcgc tgctggagat 2761 cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc gtcgacgcaa 2821 gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt tcaaaggctt 2881 ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg ccgaatacga 2941 tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc tcaagcagcg 3001 tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg cgctggttga 3061 agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg gcggtcgtta 3121 tcataatttt aaagacttta ttaatttccc caatgtcggc aaagccaatc tggtgaacaa 3181 accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca atggttttga 3241 tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg agcatgtgct 3301 ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa ttaacattta 3361 ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac ataacggtaa 3421 gaaagtcacc gtggtggttg agttacaggc gcgtttcgac gaagaagcca acattcactg 3481 ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg ggctgaaaat 3541 tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtgc gttatgcaca 3601 catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact attcgttgct 3661 gaccgccgat gcgcgcatca ccaacgaagt acggcgggta tttaacttta ttgaaaaccc 3721 ataccgtccg gtgacatttg attatttaat ggtatcgccg caaaactccc gccgcctatt 3781 gtatgaaatg gtggaccgcg agatcgccaa cgcgcagcaa gggctgccca gtggtatcac 3841 cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg cggcctccag 3901 ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc ccaatctgga 3961 aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg aacatgaccg 4021 ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg ccgactggat 4081 gacgcgcaat attgattatc gtattgaagt ggcgacgccg ctgctcgatc cgcgcctgaa 4141 gcagcgggta ctggacatca tcgacatatt gttcagcgat acggtcaaag cacgttatat 4201 cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag tacgggcgca 4261 gttggcgatt tatgactaca tcaaatcact cgaacaacct gaataatact agagtcacac 4321 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 4381 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 4441 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 4501 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 4561 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 4621 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 4681 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 4741 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 4801 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 4861 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 4921 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 4981 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 5041 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat actagagcca 5101 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 5161 tgtcggtgaa cgctctc //