##gff-version 3
##sequence-region BBa_K986030 1 5177
BBa_K986030	.	engineered_region	2158	5177	.	+	0	ID=BBa_K986028;Name=BBa_K986028
BBa_K986030	.	engineered_region	2221	5177	.	+	0	ID=BBa_S05140;Name=BBa_S05140;Parent=BBa_K986028
BBa_K986030	.	ribosome_entry_site	2221	2233	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05140
BBa_K986030	.	ribosome_entry_site	2221	2233	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986030	.	ribosome_entry_site	2227	2230	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986030	.	engineered_region	2221	2233	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986030	.	engineered_region	2240	5177	.	+	0	ID=BBa_S05137;Name=BBa_S05137;Parent=BBa_S05140
BBa_K986030	.	CDS	2240	4306	.	+	0	ID=BBa_K986000;Name=BBa_K986000;Parent=BBa_S05137
BBa_K986030	.	sequence_feature	4315	5177	.	+	0	ID=BBa_K986001;Name=BBa_K986001;Parent=BBa_S05137
BBa_K986030	.	engineered_region	4315	5089	.	+	0	ID=BBa_S05100;Name=BBa_S05100;Parent=BBa_K986001
BBa_K986030	.	CDS	4334	5089	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_S05100
BBa_K986030	.	engineered_region	4334	5053	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K986030	.	tag	5054	5086	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K986030	.	ribosome_entry_site	4315	4327	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05100
BBa_K986030	.	ribosome_entry_site	4315	4327	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986030	.	ribosome_entry_site	4321	4324	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986030	.	engineered_region	4315	4327	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986030	.	terminator	5098	5177	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K986001
BBa_K986030	.	stem_loop	5109	5152	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986030	.	engineered_region	5098	5177	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986030	.	promoter	2158	2211	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K986028
BBa_K986030	.	engineered_region	2158	2211	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K986030	.	promoter	2177	2182	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K986030	.	operator	2183	2199	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K986030	.	promoter	2200	2205	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K986030	.	operator	2160	2176	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K986030	.	engineered_region	1	2149	.	+	0	ID=BBa_K986014;Name=BBa_K986014
BBa_K986030	.	engineered_region	101	2149	.	+	0	ID=BBa_S05121;Name=BBa_S05121;Parent=BBa_K986014
BBa_K986030	.	engineered_region	101	1272	.	+	0	ID=BBa_S05101;Name=BBa_S05101;Parent=BBa_S05121
BBa_K986030	.	ribosome_entry_site	101	113	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05101
BBa_K986030	.	ribosome_entry_site	101	113	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986030	.	ribosome_entry_site	107	110	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986030	.	engineered_region	101	113	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986030	.	CDS	120	1247	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05101
BBa_K986030	.	engineered_region	120	1247	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K986030	.	tag	1209	1247	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986030	.	engineered_tag	1248	1272	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986030	.	CDS	120	1247	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986030	.	engineered_region	1281	2149	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05121
BBa_K986030	.	terminator	2109	2149	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986030	.	polyA_site	2136	2149	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986030	.	stem_loop	2116	2135	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986030	.	stop_codon	2142	2142	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986030	.	engineered_region	2109	2149	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986030	.	engineered_region	1299	2012	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986030	.	CDS	1299	2009	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986030	.	sequence_alteration	1650	1650	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986030	.	terminator	2021	2100	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986030	.	stem_loop	2032	2075	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986030	.	engineered_region	2021	2100	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986030	.	ribosome_entry_site	1281	1292	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986030	.	conserved_region	1285	1288	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986030	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023;Parent=BBa_K986014
>BBa_K986030
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagtcacacaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgca
gagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaa
aagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgct
gattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaa
ctgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcg
cgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaa
tgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcga
ctgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgc
gtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggag
tgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgat
cagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacg
acgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccag
cgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaaga
aaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggag
ttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcc
cctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccc
tcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgag
ggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacg
gcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatggg
ctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaag
gacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacg
tcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgcca
ctccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
tactagagtcacacaggaaagtactagatgggtcaggaaaagctatacatcgaaaaagagctcagttggttatcgttca
atgaacgcgtgcttcaggaagcggcggacaaatctaacccgctgattgaaaggatgcgtttcctggggatctattccaa
taaccttgatgagttctataaagtccgcttcgctgaactgaagcgacgcatcattattagcgaagaacaaggctccaac
tctcattcccgccatttactgggcaaaattcagtcccgggtgctgaaagccgatcaggagtttgacggcctctacaacg
agctattgctggagatggcgcgcaaccagatcttcctgattaatgaacgccagctctccgtcaatcaacaaaactggct
gcgtcattattttaagcagtatctgcgtcagcacattacgccgattttaatcaatcctgacactgacttagtgcagttc
ctgaaagatgattacacctatctggcggtggaaattatccgtggcgataccatccgttacgcgctgctggagatcccat
cagataaagtgccgcgctttgtgaatttaccgccagaagcgccgcgtcgacgcaagccgatgattcttctggataacat
tctgcgttactgccttgatgatattttcaaaggcttctttgattatgacgcgctgaatgcctattcaatgaagatgacc
cgcgatgccgaatacgatttagtgcatgagatggaagccagcctgatggagttgatgtcttccagtctcaagcagcgtt
taactgctgagccggtgcgttttgtttatcagcgcgatatgcccaatgcgctggttgaagtgttacgcgaaaaactgac
tatttcccgctacgactccatcgtccccggcggtcgttatcataattttaaagactttattaatttccccaatgtcggc
aaagccaatctggtgaacaaaccactgccgcgtttacgccatatttggtttgataaagcccagttccgcaatggttttg
atgccattcgcgaacgcgatgtgttgctctattatccttatcacacctttgagcatgtgctggaactgctgcgtcaggc
ttcgttcgacccgagcgtactggcgattaaaattaacatttaccgcgtggcgaaagattcacgcatcatcgactcgatg
atccacgccgcacataacggtaagaaagtcaccgtggtggttgagttacaggcgcgtttcgacgaagaagccaacattc
actgggcgaagcgcctgaccgaagcaggcgtgcacgttatcttctctgcgccggggctgaaaattcacgccaaactgtt
cctgatttcacgtaaagaaaacggtgaagtggtgcgttatgcacacatcgggaccgggaactttaacgaaaaaaccgcg
cgtctttatactgactattcgttgctgaccgccgatgcgcgcatcaccaacgaagtacggcgggtatttaactttattg
aaaacccataccgtccggtgacatttgattatttaatggtatcgccgcaaaactcccgccgcctattgtatgaaatggt
ggaccgcgagatcgccaacgcgcagcaagggctgcccagtggtatcaccctgaagctaaataaccttgtcgataaaggc
ctggttgatcgtctgtatgcggcctccagctccggcgtaccggttaatctgctggttcgcggaatgtgttcgctgatcc
ccaatctggaaggcattagcgacaacattcgtgccatcagtattgttgaccgttaccttgaacatgaccgggtttatat
ttttgaaaatggcggcgataaaaaggtctacctttcttccgccgactggatgacgcgcaatattgattatcgtattgaa
gtggcgacgccgctgctcgatccgcgcctgaagcagcgggtactggacatcatcgacatattgttcagcgatacggtca
aagcacgttatatcgataaagaactcagtaatcgctacgttccccgcggcaatcgccgcaaagtacgggcgcagttggc
gatttatgactacatcaaatcactcgaacaacctgaataatactagagtcacacaggaaagtactagatgcgtaaagga
gaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtg
gagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatg
gccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttt
ttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtg
ctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacat
tcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaa
gttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattg
gcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagag
agaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgct
gcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctc