LOCUS BBa_K986031 5176 bp DNA linear 26-Nov-2024 DEFINITION BBa_K986015+BBa_K986028 ACCESSION BBa_K986031 VERSION BBa_K986031.1 FEATURES Location/Qualifiers promoter 1..92 /label=BBa_K737023 RBS 101..112 /label=BBa_J61117 misc_feature 1208..1246 /label=LVA CDS 119..1246 /label=lacI-LVA misc_feature 119..1246 /label=BBa_C0012 misc_feature 1247..1271 /label=Help:Barcodes CDS 119..1246 /label=BBa_C0012 misc_feature 101..1271 /label=BBa_S05104 misc_feature 1284..1287 /label=conserved RBS 1280..1291 /label=BBa_B0034 CDS 1298..2008 /label=mCherry misc_feature 1649..1649 /label=C->T (removing PstI site) misc_feature 1298..2011 /label=BBa_J06504 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 misc_feature 1280..2148 /label=BBa_J06702 misc_feature 101..2148 /label=BBa_S05123 misc_feature 1..2148 /label=BBa_K986015 misc_feature 2159..2175 /label=lac O1 promoter 2176..2181 /label=-35 misc_feature 2182..2198 /label=lac O1 promoter 2199..2204 /label=-10 misc_feature 2157..2210 /label=BBa_R0011 promoter 2157..2210 /label=BBa_R0011 RBS 2220..2232 /label=RBS-3\Weak RBS 2226..2229 /label=RBS misc_feature 2220..2232 /label=BBa_B0032 RBS 2220..2232 /label=BBa_B0032 CDS 2239..4305 /label=BBa_K986000 RBS 4314..4326 /label=RBS-3\Weak RBS 4320..4323 /label=RBS misc_feature 4314..4326 /label=BBa_B0032 RBS 4314..4326 /label=BBa_B0032 misc_feature 4333..5052 /label=GFP misc_feature 5053..5085 /label=LVA CDS 4333..5088 /label=BBa_K082003 misc_feature 4314..5088 /label=BBa_S05100 stem_loop 5108..5151 /label=stem_loop misc_feature 5097..5176 /label=BBa_B0010 terminator 5097..5176 /label=BBa_B0010 misc_feature 4314..5176 /label=BBa_K986001 misc_feature 2239..5176 /label=BBa_S05137 misc_feature 2220..5176 /label=BBa_S05140 misc_feature 2157..5176 /label=BBa_K986028 ORIGIN 1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct 61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacatga gttactagat 121 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca 181 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt 241 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg 301 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca 361 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat 421 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg 481 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc 541 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag 601 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg 661 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct 721 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg 781 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt 841 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac 901 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga 961 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca 1021 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 1081 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 1141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1201 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta 1261 gtgtagatct ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga 1321 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 1381 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 1441 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 1501 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 1561 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 1621 cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa 1681 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 1741 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 1801 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 1861 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 1921 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 1981 cggcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 2161 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagagt 2221 cacacaggaa agtactagat gggtcaggaa aagctataca tcgaaaaaga gctcagttgg 2281 ttatcgttca atgaacgcgt gcttcaggaa gcggcggaca aatctaaccc gctgattgaa 2341 aggatgcgtt tcctggggat ctattccaat aaccttgatg agttctataa agtccgcttc 2401 gctgaactga agcgacgcat cattattagc gaagaacaag gctccaactc tcattcccgc 2461 catttactgg gcaaaattca gtcccgggtg ctgaaagccg atcaggagtt tgacggcctc 2521 tacaacgagc tattgctgga gatggcgcgc aaccagatct tcctgattaa tgaacgccag 2581 ctctccgtca atcaacaaaa ctggctgcgt cattatttta agcagtatct gcgtcagcac 2641 attacgccga ttttaatcaa tcctgacact gacttagtgc agttcctgaa agatgattac 2701 acctatctgg cggtggaaat tatccgtggc gataccatcc gttacgcgct gctggagatc 2761 ccatcagata aagtgccgcg ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag 2821 ccgatgattc ttctggataa cattctgcgt tactgccttg atgatatttt caaaggcttc 2881 tttgattatg acgcgctgaa tgcctattca atgaagatga cccgcgatgc cgaatacgat 2941 ttagtgcatg agatggaagc cagcctgatg gagttgatgt cttccagtct caagcagcgt 3001 ttaactgctg agccggtgcg ttttgtttat cagcgcgata tgcccaatgc gctggttgaa 3061 gtgttacgcg aaaaactgac tatttcccgc tacgactcca tcgtccccgg cggtcgttat 3121 cataatttta aagactttat taatttcccc aatgtcggca aagccaatct ggtgaacaaa 3181 ccactgccgc gtttacgcca tatttggttt gataaagccc agttccgcaa tggttttgat 3241 gccattcgcg aacgcgatgt gttgctctat tatccttatc acacctttga gcatgtgctg 3301 gaactgctgc gtcaggcttc gttcgacccg agcgtactgg cgattaaaat taacatttac 3361 cgcgtggcga aagattcacg catcatcgac tcgatgatcc acgccgcaca taacggtaag 3421 aaagtcaccg tggtggttga gttacaggcg cgtttcgacg aagaagccaa cattcactgg 3481 gcgaagcgcc tgaccgaagc aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt 3541 cacgccaaac tgttcctgat ttcacgtaaa gaaaacggtg aagtggtgcg ttatgcacac 3601 atcgggaccg ggaactttaa cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg 3661 accgccgatg cgcgcatcac caacgaagta cggcgggtat ttaactttat tgaaaaccca 3721 taccgtccgg tgacatttga ttatttaatg gtatcgccgc aaaactcccg ccgcctattg 3781 tatgaaatgg tggaccgcga gatcgccaac gcgcagcaag ggctgcccag tggtatcacc 3841 ctgaagctaa ataaccttgt cgataaaggc ctggttgatc gtctgtatgc ggcctccagc 3901 tccggcgtac cggttaatct gctggttcgc ggaatgtgtt cgctgatccc caatctggaa 3961 ggcattagcg acaacattcg tgccatcagt attgttgacc gttaccttga acatgaccgg 4021 gtttatattt ttgaaaatgg cggcgataaa aaggtctacc tttcttccgc cgactggatg 4081 acgcgcaata ttgattatcg tattgaagtg gcgacgccgc tgctcgatcc gcgcctgaag 4141 cagcgggtac tggacatcat cgacatattg ttcagcgata cggtcaaagc acgttatatc 4201 gataaagaac tcagtaatcg ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag 4261 ttggcgattt atgactacat caaatcactc gaacaacctg aataatacta gagtcacaca 4321 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 4381 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 4441 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 4501 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 4561 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 4621 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 4681 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 4741 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 4801 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 4861 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 4921 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 4981 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 5041 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata ctagagccag 5101 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 5161 gtcggtgaac gctctc //