##gff-version 3
##sequence-region BBa_K986031 1 5176
BBa_K986031	.	engineered_region	1	2148	.	+	0	ID=BBa_K986015;Name=BBa_K986015
BBa_K986031	.	engineered_region	101	2148	.	+	0	ID=BBa_S05123;Name=BBa_S05123;Parent=BBa_K986015
BBa_K986031	.	engineered_region	101	1271	.	+	0	ID=BBa_S05104;Name=BBa_S05104;Parent=BBa_S05123
BBa_K986031	.	ribosome_entry_site	101	112	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05104
BBa_K986031	.	CDS	119	1246	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S05104
BBa_K986031	.	engineered_region	119	1246	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K986031	.	engineered_tag	1247	1271	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K986031	.	tag	1208	1246	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K986031	.	CDS	119	1246	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K986031	.	engineered_region	1280	2148	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05123
BBa_K986031	.	engineered_region	1298	2011	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986031	.	CDS	1298	2008	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986031	.	sequence_alteration	1649	1649	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986031	.	ribosome_entry_site	1280	1291	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986031	.	conserved_region	1284	1287	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986031	.	terminator	2020	2099	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986031	.	engineered_region	2020	2099	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986031	.	stem_loop	2031	2074	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986031	.	terminator	2108	2148	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986031	.	stem_loop	2115	2134	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986031	.	engineered_region	2108	2148	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986031	.	polyA_site	2135	2148	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986031	.	stop_codon	2141	2141	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986031	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023;Parent=BBa_K986015
BBa_K986031	.	engineered_region	2157	5176	.	+	0	ID=BBa_K986028;Name=BBa_K986028
BBa_K986031	.	promoter	2157	2210	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K986028
BBa_K986031	.	operator	2182	2198	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K986031	.	engineered_region	2157	2210	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K986031	.	promoter	2199	2204	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K986031	.	operator	2159	2175	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K986031	.	promoter	2176	2181	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K986031	.	engineered_region	2220	5176	.	+	0	ID=BBa_S05140;Name=BBa_S05140;Parent=BBa_K986028
BBa_K986031	.	engineered_region	2239	5176	.	+	0	ID=BBa_S05137;Name=BBa_S05137;Parent=BBa_S05140
BBa_K986031	.	CDS	2239	4305	.	+	0	ID=BBa_K986000;Name=BBa_K986000;Parent=BBa_S05137
BBa_K986031	.	sequence_feature	4314	5176	.	+	0	ID=BBa_K986001;Name=BBa_K986001;Parent=BBa_S05137
BBa_K986031	.	engineered_region	4314	5088	.	+	0	ID=BBa_S05100;Name=BBa_S05100;Parent=BBa_K986001
BBa_K986031	.	ribosome_entry_site	4314	4326	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05100
BBa_K986031	.	engineered_region	4314	4326	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986031	.	ribosome_entry_site	4320	4323	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986031	.	ribosome_entry_site	4314	4326	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986031	.	CDS	4333	5088	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_S05100
BBa_K986031	.	engineered_region	4333	5052	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K986031	.	tag	5053	5085	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K986031	.	terminator	5097	5176	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K986001
BBa_K986031	.	engineered_region	5097	5176	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986031	.	stem_loop	5108	5151	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986031	.	ribosome_entry_site	2220	2232	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05140
BBa_K986031	.	engineered_region	2220	2232	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986031	.	ribosome_entry_site	2226	2229	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986031	.	ribosome_entry_site	2220	2232	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
>BBa_K986031
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagaaagacatgagttactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgt
agatctctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagt
tcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgccc
ctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccct
cagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagg
gcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacgg
cgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggc
tgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaagg
acggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgt
caacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccac
tccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacat
actagagtcacacaggaaagtactagatgggtcaggaaaagctatacatcgaaaaagagctcagttggttatcgttcaa
tgaacgcgtgcttcaggaagcggcggacaaatctaacccgctgattgaaaggatgcgtttcctggggatctattccaat
aaccttgatgagttctataaagtccgcttcgctgaactgaagcgacgcatcattattagcgaagaacaaggctccaact
ctcattcccgccatttactgggcaaaattcagtcccgggtgctgaaagccgatcaggagtttgacggcctctacaacga
gctattgctggagatggcgcgcaaccagatcttcctgattaatgaacgccagctctccgtcaatcaacaaaactggctg
cgtcattattttaagcagtatctgcgtcagcacattacgccgattttaatcaatcctgacactgacttagtgcagttcc
tgaaagatgattacacctatctggcggtggaaattatccgtggcgataccatccgttacgcgctgctggagatcccatc
agataaagtgccgcgctttgtgaatttaccgccagaagcgccgcgtcgacgcaagccgatgattcttctggataacatt
ctgcgttactgccttgatgatattttcaaaggcttctttgattatgacgcgctgaatgcctattcaatgaagatgaccc
gcgatgccgaatacgatttagtgcatgagatggaagccagcctgatggagttgatgtcttccagtctcaagcagcgttt
aactgctgagccggtgcgttttgtttatcagcgcgatatgcccaatgcgctggttgaagtgttacgcgaaaaactgact
atttcccgctacgactccatcgtccccggcggtcgttatcataattttaaagactttattaatttccccaatgtcggca
aagccaatctggtgaacaaaccactgccgcgtttacgccatatttggtttgataaagcccagttccgcaatggttttga
tgccattcgcgaacgcgatgtgttgctctattatccttatcacacctttgagcatgtgctggaactgctgcgtcaggct
tcgttcgacccgagcgtactggcgattaaaattaacatttaccgcgtggcgaaagattcacgcatcatcgactcgatga
tccacgccgcacataacggtaagaaagtcaccgtggtggttgagttacaggcgcgtttcgacgaagaagccaacattca
ctgggcgaagcgcctgaccgaagcaggcgtgcacgttatcttctctgcgccggggctgaaaattcacgccaaactgttc
ctgatttcacgtaaagaaaacggtgaagtggtgcgttatgcacacatcgggaccgggaactttaacgaaaaaaccgcgc
gtctttatactgactattcgttgctgaccgccgatgcgcgcatcaccaacgaagtacggcgggtatttaactttattga
aaacccataccgtccggtgacatttgattatttaatggtatcgccgcaaaactcccgccgcctattgtatgaaatggtg
gaccgcgagatcgccaacgcgcagcaagggctgcccagtggtatcaccctgaagctaaataaccttgtcgataaaggcc
tggttgatcgtctgtatgcggcctccagctccggcgtaccggttaatctgctggttcgcggaatgtgttcgctgatccc
caatctggaaggcattagcgacaacattcgtgccatcagtattgttgaccgttaccttgaacatgaccgggtttatatt
tttgaaaatggcggcgataaaaaggtctacctttcttccgccgactggatgacgcgcaatattgattatcgtattgaag
tggcgacgccgctgctcgatccgcgcctgaagcagcgggtactggacatcatcgacatattgttcagcgatacggtcaa
agcacgttatatcgataaagaactcagtaatcgctacgttccccgcggcaatcgccgcaaagtacgggcgcagttggcg
atttatgactacatcaaatcactcgaacaacctgaataatactagagtcacacaggaaagtactagatgcgtaaaggag
aagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgg
agagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatgg
ccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttt
tcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgc
tgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacatt
cttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaag
ttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattgg
cgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagaga
gaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctg
caaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctc