LOCUS BBa_K986032 4707 bp DNA linear 26-Nov-2024 DEFINITION BBa_K986016+BBa_K986029 ACCESSION BBa_K986032 VERSION BBa_K986032.1 FEATURES Location/Qualifiers promoter 1..92 /label=BBa_K737023 RBS 101..112 /label=BBa_J61117 CDS 122..738 /label=tetR misc_feature 739..772 /label=SsrA misc_feature 779..803 /label=Help:Barcodes CDS 119..803 /label=BBa_C0040 misc_feature 101..803 /label=BBa_S05105 misc_feature 816..819 /label=conserved RBS 812..823 /label=BBa_B0034 CDS 830..1540 /label=mCherry misc_feature 1181..1181 /label=C->T (removing PstI site) misc_feature 830..1543 /label=BBa_J06504 stem_loop 1563..1606 /label=stem_loop misc_feature 1552..1631 /label=BBa_B0010 terminator 1552..1631 /label=BBa_B0010 stem_loop 1647..1666 /label=T7 TE misc_feature 1673..1673 /label=stop polyA_site 1667..1680 /label=polya misc_feature 1640..1680 /label=BBa_B0012 terminator 1640..1680 /label=BBa_B0012 misc_feature 812..1680 /label=BBa_J06702 misc_feature 101..1680 /label=BBa_S05124 misc_feature 1..1680 /label=BBa_K986016 misc_feature 1689..1707 /label=TetR 1 misc_feature 1689..1742 /label=BBa_R0040 promoter 1708..1713 /label=-35 misc_feature 1714..1732 /label=TetR 2 promoter 1731..1736 /label=-10 promoter 1689..1742 /label=BBa_R0040 RBS 1751..1763 /label=RBS-3\Weak RBS 1757..1760 /label=RBS misc_feature 1751..1763 /label=BBa_B0032 RBS 1751..1763 /label=BBa_B0032 CDS 1770..3836 /label=BBa_K986000 RBS 3845..3857 /label=RBS-3\Weak RBS 3851..3854 /label=RBS misc_feature 3845..3857 /label=BBa_B0032 RBS 3845..3857 /label=BBa_B0032 misc_feature 3864..4583 /label=GFP misc_feature 4584..4616 /label=LVA CDS 3864..4619 /label=BBa_K082003 misc_feature 3845..4619 /label=BBa_S05100 stem_loop 4639..4682 /label=stem_loop misc_feature 4628..4707 /label=BBa_B0010 terminator 4628..4707 /label=BBa_B0010 misc_feature 3845..4707 /label=BBa_K986001 misc_feature 1770..4707 /label=BBa_S05137 misc_feature 1751..4707 /label=BBa_S05140 misc_feature 1689..4707 /label=BBa_K986029 ORIGIN 1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct 61 gacgcataat gacgtcgcat taatgatcgc aatactagag aaagacatga gttactagat 121 gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg 181 aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt 241 gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga 301 taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa 361 taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt 421 aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg 481 ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac 541 tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc 601 tactactgat agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg 661 tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact 721 taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca 781 ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga tggtgagcaa 841 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 901 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 961 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 1021 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 1081 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 1141 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat 1201 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 1261 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 1321 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 1381 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 1441 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 1501 ccgccactcc accggcggca tggacgagct gtacaagtaa taatactaga gccaggcatc 1561 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1621 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1681 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 1741 actactagag tcacacagga aagtactaga tgggtcagga aaagctatac atcgaaaaag 1801 agctcagttg gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc 1861 cgctgattga aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata 1921 aagtccgctt cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaact 1981 ctcattcccg ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggagt 2041 ttgacggcct ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta 2101 atgaacgcca gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc 2161 tgcgtcagca cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga 2221 aagatgatta cacctatctg gcggtggaaa ttatccgtgg cgataccatc cgttacgcgc 2281 tgctggagat cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc 2341 gtcgacgcaa gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt 2401 tcaaaggctt ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg 2461 ccgaatacga tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc 2521 tcaagcagcg tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg 2581 cgctggttga agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg 2641 gcggtcgtta tcataatttt aaagacttta ttaatttccc caatgtcggc aaagccaatc 2701 tggtgaacaa accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca 2761 atggttttga tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg 2821 agcatgtgct ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa 2881 ttaacattta ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac 2941 ataacggtaa gaaagtcacc gtggtggttg agttacaggc gcgtttcgac gaagaagcca 3001 acattcactg ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg 3061 ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtgc 3121 gttatgcaca catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact 3181 attcgttgct gaccgccgat gcgcgcatca ccaacgaagt acggcgggta tttaacttta 3241 ttgaaaaccc ataccgtccg gtgacatttg attatttaat ggtatcgccg caaaactccc 3301 gccgcctatt gtatgaaatg gtggaccgcg agatcgccaa cgcgcagcaa gggctgccca 3361 gtggtatcac cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg 3421 cggcctccag ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc 3481 ccaatctgga aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg 3541 aacatgaccg ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg 3601 ccgactggat gacgcgcaat attgattatc gtattgaagt ggcgacgccg ctgctcgatc 3661 cgcgcctgaa gcagcgggta ctggacatca tcgacatatt gttcagcgat acggtcaaag 3721 cacgttatat cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag 3781 tacgggcgca gttggcgatt tatgactaca tcaaatcact cgaacaacct gaataatact 3841 agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc 3901 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 3961 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 4021 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga 4081 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg 4141 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 4201 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 4261 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 4321 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 4381 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 4441 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 4501 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 4561 tggatgaact atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 4621 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 4681 atctgttgtt tgtcggtgaa cgctctc //