##gff-version 3
##sequence-region BBa_K986032 1 4707
BBa_K986032	.	engineered_region	1689	4707	.	+	0	ID=BBa_K986029;Name=BBa_K986029
BBa_K986032	.	engineered_region	1751	4707	.	+	0	ID=BBa_S05140;Name=BBa_S05140;Parent=BBa_K986029
BBa_K986032	.	engineered_region	1770	4707	.	+	0	ID=BBa_S05137;Name=BBa_S05137;Parent=BBa_S05140
BBa_K986032	.	CDS	1770	3836	.	+	0	ID=BBa_K986000;Name=BBa_K986000;Parent=BBa_S05137
BBa_K986032	.	sequence_feature	3845	4707	.	+	0	ID=BBa_K986001;Name=BBa_K986001;Parent=BBa_S05137
BBa_K986032	.	terminator	4628	4707	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K986001
BBa_K986032	.	stem_loop	4639	4682	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986032	.	engineered_region	4628	4707	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986032	.	engineered_region	3845	4619	.	+	0	ID=BBa_S05100;Name=BBa_S05100;Parent=BBa_K986001
BBa_K986032	.	CDS	3864	4619	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_S05100
BBa_K986032	.	engineered_region	3864	4583	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K986032	.	tag	4584	4616	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K986032	.	ribosome_entry_site	3845	3857	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05100
BBa_K986032	.	engineered_region	3845	3857	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986032	.	ribosome_entry_site	3851	3854	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986032	.	ribosome_entry_site	3845	3857	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986032	.	ribosome_entry_site	1751	1763	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05140
BBa_K986032	.	engineered_region	1751	1763	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986032	.	ribosome_entry_site	1757	1760	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986032	.	ribosome_entry_site	1751	1763	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986032	.	promoter	1689	1742	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K986029
BBa_K986032	.	engineered_region	1689	1742	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K986032	.	promoter	1708	1713	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K986032	.	non_covalent_binding_site	1714	1732	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K986032	.	promoter	1731	1736	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K986032	.	non_covalent_binding_site	1689	1707	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K986032	.	engineered_region	1	1680	.	+	0	ID=BBa_K986016;Name=BBa_K986016
BBa_K986032	.	engineered_region	101	1680	.	+	0	ID=BBa_S05124;Name=BBa_S05124;Parent=BBa_K986016
BBa_K986032	.	engineered_region	101	803	.	+	0	ID=BBa_S05105;Name=BBa_S05105;Parent=BBa_S05124
BBa_K986032	.	ribosome_entry_site	101	112	.	+	0	ID=BBa_J61117;Name=BBa_J61117;Parent=BBa_S05105
BBa_K986032	.	CDS	119	803	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05105
BBa_K986032	.	engineered_tag	779	803	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K986032	.	CDS	122	738	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K986032	.	tag	739	772	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K986032	.	engineered_region	812	1680	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05124
BBa_K986032	.	engineered_region	830	1543	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986032	.	CDS	830	1540	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986032	.	sequence_alteration	1181	1181	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986032	.	terminator	1552	1631	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986032	.	stem_loop	1563	1606	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986032	.	engineered_region	1552	1631	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986032	.	ribosome_entry_site	812	823	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986032	.	conserved_region	816	819	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986032	.	terminator	1640	1680	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986032	.	stem_loop	1647	1666	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986032	.	polyA_site	1667	1680	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986032	.	stop_codon	1673	1673	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986032	.	engineered_region	1640	1680	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986032	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023;Parent=BBa_K986016
>BBa_K986032
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagaaagacatgagttactagatgtccagattagataaaagtaaagtgattaacagcgcat
tagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctac
attgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcac
ttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtc
atcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctt
tttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgta
ttggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgac
aagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattaga
aaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgc
tagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatca
aggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgaggg
ccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctg
tcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttcc
ccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgca
ggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagacc
atgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagc
tgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgccta
caacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggc
cgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagtcacacaggaaagtactagatgggtcaggaaaagctatacatcgaaaaagagctcagttggttatcg
ttcaatgaacgcgtgcttcaggaagcggcggacaaatctaacccgctgattgaaaggatgcgtttcctggggatctatt
ccaataaccttgatgagttctataaagtccgcttcgctgaactgaagcgacgcatcattattagcgaagaacaaggctc
caactctcattcccgccatttactgggcaaaattcagtcccgggtgctgaaagccgatcaggagtttgacggcctctac
aacgagctattgctggagatggcgcgcaaccagatcttcctgattaatgaacgccagctctccgtcaatcaacaaaact
ggctgcgtcattattttaagcagtatctgcgtcagcacattacgccgattttaatcaatcctgacactgacttagtgca
gttcctgaaagatgattacacctatctggcggtggaaattatccgtggcgataccatccgttacgcgctgctggagatc
ccatcagataaagtgccgcgctttgtgaatttaccgccagaagcgccgcgtcgacgcaagccgatgattcttctggata
acattctgcgttactgccttgatgatattttcaaaggcttctttgattatgacgcgctgaatgcctattcaatgaagat
gacccgcgatgccgaatacgatttagtgcatgagatggaagccagcctgatggagttgatgtcttccagtctcaagcag
cgtttaactgctgagccggtgcgttttgtttatcagcgcgatatgcccaatgcgctggttgaagtgttacgcgaaaaac
tgactatttcccgctacgactccatcgtccccggcggtcgttatcataattttaaagactttattaatttccccaatgt
cggcaaagccaatctggtgaacaaaccactgccgcgtttacgccatatttggtttgataaagcccagttccgcaatggt
tttgatgccattcgcgaacgcgatgtgttgctctattatccttatcacacctttgagcatgtgctggaactgctgcgtc
aggcttcgttcgacccgagcgtactggcgattaaaattaacatttaccgcgtggcgaaagattcacgcatcatcgactc
gatgatccacgccgcacataacggtaagaaagtcaccgtggtggttgagttacaggcgcgtttcgacgaagaagccaac
attcactgggcgaagcgcctgaccgaagcaggcgtgcacgttatcttctctgcgccggggctgaaaattcacgccaaac
tgttcctgatttcacgtaaagaaaacggtgaagtggtgcgttatgcacacatcgggaccgggaactttaacgaaaaaac
cgcgcgtctttatactgactattcgttgctgaccgccgatgcgcgcatcaccaacgaagtacggcgggtatttaacttt
attgaaaacccataccgtccggtgacatttgattatttaatggtatcgccgcaaaactcccgccgcctattgtatgaaa
tggtggaccgcgagatcgccaacgcgcagcaagggctgcccagtggtatcaccctgaagctaaataaccttgtcgataa
aggcctggttgatcgtctgtatgcggcctccagctccggcgtaccggttaatctgctggttcgcggaatgtgttcgctg
atccccaatctggaaggcattagcgacaacattcgtgccatcagtattgttgaccgttaccttgaacatgaccgggttt
atatttttgaaaatggcggcgataaaaaggtctacctttcttccgccgactggatgacgcgcaatattgattatcgtat
tgaagtggcgacgccgctgctcgatccgcgcctgaagcagcgggtactggacatcatcgacatattgttcagcgatacg
gtcaaagcacgttatatcgataaagaactcagtaatcgctacgttccccgcggcaatcgccgcaaagtacgggcgcagt
tggcgatttatgactacatcaaatcactcgaacaacctgaataatactagagtcacacaggaaagtactagatgcgtaa
aggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttc
catggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaa
acattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaat
caaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcca
attggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaa
agagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcc
tgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctc