LOCUS BBa_K986034 4708 bp DNA linear 29-Jun-2024 DEFINITION BBa_K986020+BBa_K986029 ACCESSION BBa_K986034 VERSION BBa_K986034.1 FEATURES Location/Qualifiers promoter 1..92 /label=BBa_K737023 RBS 101..113 /label=RBS-3\Weak RBS 107..110 /label=RBS misc_feature 101..113 /label=BBa_B0032 RBS 101..113 /label=BBa_B0032 CDS 123..739 /label=tetR misc_feature 740..773 /label=SsrA misc_feature 780..804 /label=Help:Barcodes CDS 120..804 /label=BBa_C0040 misc_feature 101..804 /label=BBa_S05102 misc_feature 817..820 /label=conserved RBS 813..824 /label=BBa_B0034 CDS 831..1541 /label=mCherry misc_feature 1182..1182 /label=C->T (removing PstI site) misc_feature 831..1544 /label=BBa_J06504 stem_loop 1564..1607 /label=stem_loop misc_feature 1553..1632 /label=BBa_B0010 terminator 1553..1632 /label=BBa_B0010 stem_loop 1648..1667 /label=T7 TE misc_feature 1674..1674 /label=stop polyA_site 1668..1681 /label=polya misc_feature 1641..1681 /label=BBa_B0012 terminator 1641..1681 /label=BBa_B0012 misc_feature 813..1681 /label=BBa_J06702 misc_feature 101..1681 /label=BBa_S05122 misc_feature 1..1681 /label=BBa_K986020 misc_feature 1690..1708 /label=TetR 1 misc_feature 1690..1743 /label=BBa_R0040 promoter 1709..1714 /label=-35 misc_feature 1715..1733 /label=TetR 2 promoter 1732..1737 /label=-10 promoter 1690..1743 /label=BBa_R0040 RBS 1752..1764 /label=RBS-3\Weak RBS 1758..1761 /label=RBS misc_feature 1752..1764 /label=BBa_B0032 RBS 1752..1764 /label=BBa_B0032 CDS 1771..3837 /label=BBa_K986000 RBS 3846..3858 /label=RBS-3\Weak RBS 3852..3855 /label=RBS misc_feature 3846..3858 /label=BBa_B0032 RBS 3846..3858 /label=BBa_B0032 misc_feature 3865..4584 /label=GFP misc_feature 4585..4617 /label=LVA CDS 3865..4620 /label=BBa_K082003 misc_feature 3846..4620 /label=BBa_S05100 stem_loop 4640..4683 /label=stem_loop misc_feature 4629..4708 /label=BBa_B0010 terminator 4629..4708 /label=BBa_B0010 misc_feature 3846..4708 /label=BBa_K986001 misc_feature 1771..4708 /label=BBa_S05137 misc_feature 1752..4708 /label=BBa_S05140 misc_feature 1690..4708 /label=BBa_K986029 ORIGIN 1 acggaaatca ataacctgaa gatatgtgcg acgagctttt cataaatctg tcataaatct 61 gacgcataat gacgtcgcat taatgatcgc aatactagag tcacacagga aagtactaga 121 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg 181 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat 241 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag 301 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta 361 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt 421 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat 481 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta 541 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac 601 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag 661 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac 721 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac 781 actgatagtg ctagtgtaga tcactactag agaaagagga gaaatactag atggtgagca 841 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 901 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 961 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 1021 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 1081 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 1141 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cttgcaggac ggcgagttca 1201 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 1261 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 1321 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 1381 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 1441 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 1501 gccgccactc caccggcggc atggacgagc tgtacaagta ataatactag agccaggcat 1561 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1621 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1681 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag 1741 cactactaga gtcacacagg aaagtactag atgggtcagg aaaagctata catcgaaaaa 1801 gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac 1861 ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat 1921 aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac 1981 tctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggag 2041 tttgacggcc tctacaacga gctattgctg gagatggcgc gcaaccagat cttcctgatt 2101 aatgaacgcc agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat 2161 ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg 2221 aaagatgatt acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg 2281 ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcgccg 2341 cgtcgacgca agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt 2401 ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat 2461 gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt 2521 ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat 2581 gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc 2641 ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat 2701 ctggtgaaca aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc 2761 aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt 2821 gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa 2881 attaacattt accgcgtggc gaaagattca cgcatcatcg actcgatgat ccacgccgca 2941 cataacggta agaaagtcac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc 3001 aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg 3061 gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtg 3121 cgttatgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac 3181 tattcgttgc tgaccgccga tgcgcgcatc accaacgaag tacggcgggt atttaacttt 3241 attgaaaacc cataccgtcc ggtgacattt gattatttaa tggtatcgcc gcaaaactcc 3301 cgccgcctat tgtatgaaat ggtggaccgc gagatcgcca acgcgcagca agggctgccc 3361 agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat 3421 gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc 3481 cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt 3541 gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc 3601 gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacgcc gctgctcgat 3661 ccgcgcctga agcagcgggt actggacatc atcgacatat tgttcagcga tacggtcaaa 3721 gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa 3781 gtacgggcgc agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataatac 3841 tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc 3901 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag 3961 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa 4021 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg 4081 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat 4141 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc 4201 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa 4261 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc 4321 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa 4381 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct 4441 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac 4501 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc 4561 atggatgaac tatacaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 4621 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 4681 tatctgttgt ttgtcggtga acgctctc //