##gff-version 3
##sequence-region BBa_K986034 1 4708
BBa_K986034	.	engineered_region	1690	4708	.	+	0	ID=BBa_K986029;Name=BBa_K986029
BBa_K986034	.	promoter	1690	1743	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K986029
BBa_K986034	.	promoter	1732	1737	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K986034	.	non_covalent_binding_site	1715	1733	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K986034	.	non_covalent_binding_site	1690	1708	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K986034	.	promoter	1709	1714	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K986034	.	engineered_region	1690	1743	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K986034	.	engineered_region	1752	4708	.	+	0	ID=BBa_S05140;Name=BBa_S05140;Parent=BBa_K986029
BBa_K986034	.	engineered_region	1771	4708	.	+	0	ID=BBa_S05137;Name=BBa_S05137;Parent=BBa_S05140
BBa_K986034	.	sequence_feature	3846	4708	.	+	0	ID=BBa_K986001;Name=BBa_K986001;Parent=BBa_S05137
BBa_K986034	.	engineered_region	3846	4620	.	+	0	ID=BBa_S05100;Name=BBa_S05100;Parent=BBa_K986001
BBa_K986034	.	ribosome_entry_site	3846	3858	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05100
BBa_K986034	.	ribosome_entry_site	3852	3855	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986034	.	ribosome_entry_site	3846	3858	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986034	.	engineered_region	3846	3858	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986034	.	CDS	3865	4620	.	+	0	ID=BBa_K082003;Name=BBa_K082003;Parent=BBa_S05100
BBa_K986034	.	engineered_region	3865	4584	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K986034	.	tag	4585	4617	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K986034	.	terminator	4629	4708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K986001
BBa_K986034	.	engineered_region	4629	4708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986034	.	stem_loop	4640	4683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K986034	.	CDS	1771	3837	.	+	0	ID=BBa_K986000;Name=BBa_K986000;Parent=BBa_S05137
BBa_K986034	.	ribosome_entry_site	1752	1764	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05140
BBa_K986034	.	ribosome_entry_site	1758	1761	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986034	.	ribosome_entry_site	1752	1764	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986034	.	engineered_region	1752	1764	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986034	.	engineered_region	1	1681	.	+	0	ID=BBa_K986020;Name=BBa_K986020
BBa_K986034	.	promoter	1	92	.	+	0	ID=BBa_K737023;Name=BBa_K737023;Parent=BBa_K986020
BBa_K986034	.	engineered_region	101	1681	.	+	0	ID=BBa_S05122;Name=BBa_S05122;Parent=BBa_K986020
BBa_K986034	.	engineered_region	101	804	.	+	0	ID=BBa_S05102;Name=BBa_S05102;Parent=BBa_S05122
BBa_K986034	.	CDS	120	804	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S05102
BBa_K986034	.	tag	740	773	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K986034	.	engineered_tag	780	804	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K986034	.	CDS	123	739	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K986034	.	ribosome_entry_site	101	113	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S05102
BBa_K986034	.	ribosome_entry_site	107	110	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K986034	.	ribosome_entry_site	101	113	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K986034	.	engineered_region	101	113	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K986034	.	engineered_region	813	1681	.	+	0	ID=BBa_J06702;Name=BBa_J06702;Parent=BBa_S05122
BBa_K986034	.	ribosome_entry_site	813	824	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J06702
BBa_K986034	.	conserved_region	817	820	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K986034	.	terminator	1641	1681	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J06702
BBa_K986034	.	stem_loop	1648	1667	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K986034	.	stop_codon	1674	1674	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K986034	.	engineered_region	1641	1681	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K986034	.	polyA_site	1668	1681	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K986034	.	engineered_region	831	1544	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_J06702
BBa_K986034	.	CDS	831	1541	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K986034	.	sequence_alteration	1182	1182	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K986034	.	terminator	1553	1632	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J06702
BBa_K986034	.	engineered_region	1553	1632	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K986034	.	stem_loop	1564	1607	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K986034
acggaaatcaataacctgaagatatgtgcgacgagcttttcataaatctgtcataaatctgacgcataatgacgtcgca
ttaatgatcgcaatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgca
ttagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagccta
cattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactca
cttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagt
catcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcct
ttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgt
attggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacga
caagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattag
aaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtg
ctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcct
acaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgaggg
ccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactg
agcactactagagtcacacaggaaagtactagatgggtcaggaaaagctatacatcgaaaaagagctcagttggttatc
gttcaatgaacgcgtgcttcaggaagcggcggacaaatctaacccgctgattgaaaggatgcgtttcctggggatctat
tccaataaccttgatgagttctataaagtccgcttcgctgaactgaagcgacgcatcattattagcgaagaacaaggct
ccaactctcattcccgccatttactgggcaaaattcagtcccgggtgctgaaagccgatcaggagtttgacggcctcta
caacgagctattgctggagatggcgcgcaaccagatcttcctgattaatgaacgccagctctccgtcaatcaacaaaac
tggctgcgtcattattttaagcagtatctgcgtcagcacattacgccgattttaatcaatcctgacactgacttagtgc
agttcctgaaagatgattacacctatctggcggtggaaattatccgtggcgataccatccgttacgcgctgctggagat
cccatcagataaagtgccgcgctttgtgaatttaccgccagaagcgccgcgtcgacgcaagccgatgattcttctggat
aacattctgcgttactgccttgatgatattttcaaaggcttctttgattatgacgcgctgaatgcctattcaatgaaga
tgacccgcgatgccgaatacgatttagtgcatgagatggaagccagcctgatggagttgatgtcttccagtctcaagca
gcgtttaactgctgagccggtgcgttttgtttatcagcgcgatatgcccaatgcgctggttgaagtgttacgcgaaaaa
ctgactatttcccgctacgactccatcgtccccggcggtcgttatcataattttaaagactttattaatttccccaatg
tcggcaaagccaatctggtgaacaaaccactgccgcgtttacgccatatttggtttgataaagcccagttccgcaatgg
ttttgatgccattcgcgaacgcgatgtgttgctctattatccttatcacacctttgagcatgtgctggaactgctgcgt
caggcttcgttcgacccgagcgtactggcgattaaaattaacatttaccgcgtggcgaaagattcacgcatcatcgact
cgatgatccacgccgcacataacggtaagaaagtcaccgtggtggttgagttacaggcgcgtttcgacgaagaagccaa
cattcactgggcgaagcgcctgaccgaagcaggcgtgcacgttatcttctctgcgccggggctgaaaattcacgccaaa
ctgttcctgatttcacgtaaagaaaacggtgaagtggtgcgttatgcacacatcgggaccgggaactttaacgaaaaaa
ccgcgcgtctttatactgactattcgttgctgaccgccgatgcgcgcatcaccaacgaagtacggcgggtatttaactt
tattgaaaacccataccgtccggtgacatttgattatttaatggtatcgccgcaaaactcccgccgcctattgtatgaa
atggtggaccgcgagatcgccaacgcgcagcaagggctgcccagtggtatcaccctgaagctaaataaccttgtcgata
aaggcctggttgatcgtctgtatgcggcctccagctccggcgtaccggttaatctgctggttcgcggaatgtgttcgct
gatccccaatctggaaggcattagcgacaacattcgtgccatcagtattgttgaccgttaccttgaacatgaccgggtt
tatatttttgaaaatggcggcgataaaaaggtctacctttcttccgccgactggatgacgcgcaatattgattatcgta
ttgaagtggcgacgccgctgctcgatccgcgcctgaagcagcgggtactggacatcatcgacatattgttcagcgatac
ggtcaaagcacgttatatcgataaagaactcagtaatcgctacgttccccgcggcaatcgccgcaaagtacgggcgcag
ttggcgatttatgactacatcaaatcactcgaacaacctgaataatactagagtcacacaggaaagtactagatgcgta
aaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgt
cagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgtt
ccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatg
actttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagac
acgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgga
aacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaa
tcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactcc
aattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaa
aagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggc
ctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc