BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_K314100 1 BBa_K314100 High constitutive expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 High constitutive protein expression insert includes f1 origin and RBS B0034 false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2090995 1 BBa_K314110 annotation2090995 1 BBa_K314110 range2090995 1 1 455 BBa_K988000 1 BBa_K988000 K314100_E.coli PELB signal sequence_OXT_His-tag_double stop codon_pSB1C3 2013-06-20T11:00:00Z 2015-05-08T01:13:50Z This sequence was optimized from the human Oxytocin gene. We have a high efficiency constitutive promoter and RBS preceding the signal sequence for maximum production. We used the PELB signal sequence from E. coli to guide the protein into the periplasmic space. We added a histidine tag to the end of the Oxytocin-NeurophysinI gene. This protein is also known as prepro-oxyphysin. false false _1285_ 0 12610 9 In stock false We used the PELB signal sequence from E. coli because the Oxytocin protein begins with a cysteine that forms a disulfide bond with the cysteine in position six. This cysteine must be free of any other amino acids. The PELB signal sequence guides the protein to the inner membrane of E. coli and the signal sequence is cleaved as the protein enters the periplasmic space, leaving the position one cysteine free to form disulfide bonds. false Erin Kelly component2329951 1 BBa_K314100 annotation2329951 1 BBa_K314100 range2329951 1 1 518 BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_K314100_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa BBa_K988000_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z