BBa_K314100 1 BBa_K314100 High constitutive expression cassette 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z construct Released HQ 2013 High constitutive protein expression insert includes f1 origin and RBS B0034 false false _496_ 0 6395 9 In stock true false Tomas Huber, Justin Siegel component2090995 1 BBa_K314110 annotation2090995 1 BBa_K314110 range2090995 1 1 455 BBa_K988003 1 BBa_K988003 K314100+K988002 2013-06-20T11:00:00Z 2015-05-08T01:13:50Z http://parts.igem.org/Part:BBa_K314100 http://parts.igem.org/Part:BBa_K988002 Composite of(BBa_k314100 http://parts.igem.org/Part:BBa_K314100) F1 phage origin, Promoter J23100, and Ribosomal Binding Site B0034 this has been ligated with Oxytocin-Neurophysin (http://parts.igem.org/Part:BBa_K988002) false false _1285_ 0 12948 9 Not in stock false Their is a lack of inducible promoters with RBS in the registry, so an constitutive promoter was used instead to express the protein. false Isaac Ward component2330016 1 BBa_K314100 component2330021 1 BBa_K988002 annotation2330016 1 BBa_K314100 range2330016 1 1 518 annotation2330021 1 BBa_K988002 range2330021 1 527 932 BBa_K314110 1 f1 ori f1 origin 2010-10-07T11:00:00Z 2015-05-08T01:11:55Z Pet 29 vector Released HQ 2013 Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage false false _496_ 0 6395 9 In stock true false IGEM 2010 Team Washington annotation2115768 1 f1 origin range2115768 1 18 324 BBa_K988002 1 BBa_K988002 Oxytocin-Neurophysin1 2013-06-20T11:00:00Z 2015-05-08T01:13:50Z This Part was developed from the Homosapien sapien coding sequence for Oxytocin-Neurophysin1 This Part was developed from the Homosapien sapien (human) coding sequence for Oxytocin-Neurophysin1(1) the original signal sequence was replaced with a new (PelB) signal sequence (2) to send the protein to periplasmic space on and then be cleaved the 5' end this also allows for potential increased stability of the folded protein. A 6x-His tag was added for purification on the 3' end. (1)http://www.uniprot.org/uniprot/P01178 (2)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2259416/ (3)http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063442 false false _1285_ 0 12948 9 It's complicated false The part was back translated from the protein sequence to ensure the right DNA sequence was obtained for the proper protein sequence. When adding the PelB signal sequence it was was very important to consider the cleavage site as the C-C disulfide bond was necessary to be translated, without a signal sequence the protein would have to start with a Methionine, with would disrupt the C-C disulfide bond. the His tag was added to help with with protein Purification. false Isaac Ward annotation2329967 1 Signal Sequence range2329967 1 2 67 annotation2329990 1 Oxytocin range2329990 1 68 95 annotation2329991 1 Neurophysin 1 range2329991 1 96 386 annotation2329992 1 6x Histidine tag & stop codon range2329992 1 386 406 BBa_K314110_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt BBa_K988003_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagagtatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctgctacatccagaactgcccgctgggtggtaaacgtgctgctccggacctggacgttcgtaaatgcctgccgtgcggtccgggtggtaaaggtcgttgcttcggtccgaacatctgctgcgctgaagaactgggttgcttcgttggtaccgctgaagctctgcgttgccaggaagaaaactacctgccgtctccgtgccagtctggtcagaaagcttgcggttctggtggtcgttgcgctgttctgggtctgtgctgctctccggacggttgccacgctgacccggcttgcgacgctgaagctaccttctctcagcgtcaccaccaccaccaccactaa BBa_K988002_sequence 1 tatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctgctacatccagaactgcccgctgggtggtaaacgtgctgctccggacctggacgttcgtaaatgcctgccgtgcggtccgggtggtaaaggtcgttgcttcggtccgaacatctgctgcgctgaagaactgggttgcttcgttggtaccgctgaagctctgcgttgccaggaagaaaactacctgccgtctccgtgccagtctggtcagaaagcttgcggttctggtggtcgttgcgctgttctgggtctgtgctgctctccggacggttgccacgctgacccggcttgcgacgctgaagctaccttctctcagcgtcaccaccaccaccaccactaa BBa_K314100_sequence 1 acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z