LOCUS       BBa_K990001             2742 bp    DNA     linear       08-Apr-2025
DEFINITION  pOmpC+EPIC Firefly Luciferase
ACCESSION   BBa_K990001
VERSION     BBa_K990001.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     RBS             117..133
                     /label=rbs
     CDS             134..1057
                     /label=Luciferin regenerating enzyme (P.pyralis)
     misc_feature    1058..1063
                     /label=double stop codon
     misc_feature    1064..1069
                     /label=BamH1 site
     RBS             1070..1086
                     /label=rbs
     misc_feature    1087..2736
                     /label=EPIC luciferase (P. pyralis)
     misc_feature    2737..2742
                     /label=double stop codon
     misc_feature    117..2742
                     /label=BBa_K325100
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagttaa
      121 ggaggtaaaa aaaatgggtc cggtcgttga aaagatcgcc gagctgggta agtacacggt
      181 tggtgagggt ccgcactggg accacgaaac gcagacgctg tatttcgtgg atacggtcga
      241 gaaaacgttc cacaaatacg ttccgagcca gaagaaatac acgttttgta aggtggacaa
      301 gctggttagc tttatcattc cgctggcagg ttctccgggt cgcttcgtcg tcagcttgga
      361 gcgcgagatt gcgatcctga cgtgggacgg cgtcagcgcg gcaccgacca gcattgaggc
      421 gattgtgaat gtcgagccgc acatcaagaa taatcgcctg aacgacggta aagcggaccc
      481 gctgggtaat ctgtggaccg gcacgatggc cattgatgcg ggtttgccgg tgggtccggt
      541 gaccggctcc ctgtaccacc tgggtgcgga taagaaggtc aagatgcatg aaagcaatat
      601 tgccatcgct aacggcctgg cgtggagcaa cgacctgaag aagatgtact atattgacag
      661 cggcaaacgt cgtgttgacg aatacgacta tgatgcgagc acgctgtcta tcagcaatca
      721 gcgtccgctg ttcaccttcg aaaaacatga ggtccctggc tacccagacg gtcaaaccat
      781 tgacgaagaa ggtaacctgt gggtcgccgt gtttcaaggc cagcgtatta tcaagattag
      841 cacccagcag ccggaggttc tgctggatac cgtcaaaatc ccggacccgc aggtgactag
      901 cgttgcattc ggtggtccga atctggacga gctgcatgtg accagcgccg gtttgcagct
      961 ggatgacagc tccctggaca aatctttggt caatggtcac gtctaccgtg tgactggcct
     1021 gggtgttaag ggtttcgccg gtgtgaaagt taagctgtaa taaggatcct taaggaggta
     1081 aaaaaaatgg aggacgcgaa aaacatcaag aaaggcccag caccgttcta tccgctggaa
     1141 gatggtaccg ccggtgaaca attgcacaaa gcgatgaaac gttatgcgct ggttccgggt
     1201 accatcgcat tcacggacgc gcatatcgag gttaacatca cctatgccga gtacttcgag
     1261 atgagcgttc gtctggccga ggcaatgaag cgttatggtt tgaataccaa tcatcgcatc
     1321 gtcgtctgta gcgagaacag cttgcagttt ttcatgccag ttctgggtgc actgtttatt
     1381 ggtgtggcag tggcgcctgc gaatgatatc tacaacgagc gtgaactgtt gaatagcatg
     1441 aacatttcgc agccgacggt tgtttttgtg tccaagaaag gtttgcagaa gattctgaat
     1501 gtgcaaaaga agttgccgat cattcagaaa atcatcatca tggacagcaa gaccgattac
     1561 cagggtttcc aaagcatgta cacttttgtg acgagccacc tgcctccggg ttttaacgag
     1621 tatgatttcg ttccggaaag cttcgaccgt gataagacga ttgcactgat tatgaactcg
     1681 tccggctcta ccggcctgcc taaaggcgtg gccctgccgc accgtactgc atgtgtccgc
     1741 ttcagccacg cgcgtgatcc gatcttcggt aaccaaatca ttccggacac cgccattttg
     1801 tctgtcgttc cgtttcatca tggctttggt atgtttacca ctctgggtta cctgatctgt
     1861 ggtttccgtg ttgttctgat gtaccgtttc gaagaagaac tgttcctgcg tagcctgcaa
     1921 gattacaaga tccaatccgc gctgctggtg ccgacgctgt ttagcttctt cgctaaatcc
     1981 accctgattg acaaatatga cttgagcaac ctgcacgaaa tcgcgtcggg cggtgcgccg
     2041 ctgagcaaag aagtcggtga ggccgttgcg aaacgtttcc atctgccggg catccgtcag
     2101 ggttatggcc tgacggaaac gaccagcgcg attttgatta cccctgaggg tgacgataaa
     2161 ccgggtgccg ttggcaaggt ggtaccgttc tttgaggcta aagtcgtgga cctggacacg
     2221 ggtaagactc tgggtgtgaa tcaacgtggc gagttgtgcg ttcgtggccc gatgatcatg
     2281 agcggttatg tcaacaaccc ggaagcaact aatgcgttga tcgataagga tggctggctg
     2341 cacagcggcg acctggcgta ctgggatgag gatgaacact ttttcatcgt tggtcgcctg
     2401 aagagcctga tcaagtacaa gggctaccag gtggcaccgg ctgaactgga gagcattctg
     2461 ttgcaacacc ctaatatctt cgacgcgggt gttgcgggtc tgcctgatga tgatgccggt
     2521 gaattgccag ctgcggtggt cgttctggaa cacggtaaaa cgatgactga aaaagaaatc
     2581 gtggactacg tcgcctcgca ggtgaccacc gctaaaaagt tgcgtggtgg cgtggtgttt
     2641 gtggatgaag tgccgaaagg cctgacgggt aaacgtgatg cacgtaagat tcgtgagatc
     2701 ttgatcaagg cgaagaaagg tggtaaatcc aagttgtaat aa
//