BBa_K325100 1 BBa_K325100 EPIC Firefly Luciferase and LRE <BR><i>P. Pyralis<BR>(E. coli optimised)</i> 2010-09-14T11:00:00Z 2015-05-08T01:12:02Z Codon optimised and synthesised by DNA 2.0 Released HQ 2013 This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate. false false _446_ 0 7466 9 In stock false It was ensured that no unwanted restriction sites were created during optimisation. A BamH1 site was added between the two genes. false Ben Reeve and Paul Masset annotation2080159 1 BamH1 site range2080159 1 948 953 annotation2080158 1 double stop codon range2080158 1 942 947 annotation2080161 1 EPIC luciferase (P. pyralis) range2080161 1 971 2620 annotation2080157 1 Luciferin regenerating enzyme (P.pyralis) range2080157 1 18 941 annotation2080156 1 rbs range2080156 1 1 17 annotation2080162 1 double stop codon range2080162 1 2621 2626 annotation2080160 1 rbs range2080160 1 954 970 BBa_K990001 1 BBa_K990001 pOmpC+EPIC Firefly Luciferase 2013-06-20T11:00:00Z 2015-05-08T01:13:50Z EPIC Luciferase: BBa_K325100, Cambridge 2010 pOmpC: BBa_R0082 This part is the one of the responding systems of the receiver device of our Photocommunication system false false _1287_ 0 11713 9 It's complicated true We used ncbi when we couldn't find sequence false Fatma Bet??l ??EV&#304;K component2329858 1 BBa_K325100 component2329850 1 BBa_R0082 annotation2329850 1 BBa_R0082 range2329850 1 1 108 annotation2329858 1 BBa_K325100 range2329858 1 117 2742 BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301155 1 C2 OmpR range301155 1 34 51 annotation301156 1 C3 OmpR range301156 1 54 71 annotation301154 1 C1 OmpR range301154 1 13 30 annotation301166 1 -35 range301166 1 75 80 annotation301167 1 -10 range301167 1 98 103 BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_K990001_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagttaaggaggtaaaaaaaatgggtccggtcgttgaaaagatcgccgagctgggtaagtacacggttggtgagggtccgcactgggaccacgaaacgcagacgctgtatttcgtggatacggtcgagaaaacgttccacaaatacgttccgagccagaagaaatacacgttttgtaaggtggacaagctggttagctttatcattccgctggcaggttctccgggtcgcttcgtcgtcagcttggagcgcgagattgcgatcctgacgtgggacggcgtcagcgcggcaccgaccagcattgaggcgattgtgaatgtcgagccgcacatcaagaataatcgcctgaacgacggtaaagcggacccgctgggtaatctgtggaccggcacgatggccattgatgcgggtttgccggtgggtccggtgaccggctccctgtaccacctgggtgcggataagaaggtcaagatgcatgaaagcaatattgccatcgctaacggcctggcgtggagcaacgacctgaagaagatgtactatattgacagcggcaaacgtcgtgttgacgaatacgactatgatgcgagcacgctgtctatcagcaatcagcgtccgctgttcaccttcgaaaaacatgaggtccctggctacccagacggtcaaaccattgacgaagaaggtaacctgtgggtcgccgtgtttcaaggccagcgtattatcaagattagcacccagcagccggaggttctgctggataccgtcaaaatcccggacccgcaggtgactagcgttgcattcggtggtccgaatctggacgagctgcatgtgaccagcgccggtttgcagctggatgacagctccctggacaaatctttggtcaatggtcacgtctaccgtgtgactggcctgggtgttaagggtttcgccggtgtgaaagttaagctgtaataaggatccttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa BBa_K325100_sequence 1 ttaaggaggtaaaaaaaatgggtccggtcgttgaaaagatcgccgagctgggtaagtacacggttggtgagggtccgcactgggaccacgaaacgcagacgctgtatttcgtggatacggtcgagaaaacgttccacaaatacgttccgagccagaagaaatacacgttttgtaaggtggacaagctggttagctttatcattccgctggcaggttctccgggtcgcttcgtcgtcagcttggagcgcgagattgcgatcctgacgtgggacggcgtcagcgcggcaccgaccagcattgaggcgattgtgaatgtcgagccgcacatcaagaataatcgcctgaacgacggtaaagcggacccgctgggtaatctgtggaccggcacgatggccattgatgcgggtttgccggtgggtccggtgaccggctccctgtaccacctgggtgcggataagaaggtcaagatgcatgaaagcaatattgccatcgctaacggcctggcgtggagcaacgacctgaagaagatgtactatattgacagcggcaaacgtcgtgttgacgaatacgactatgatgcgagcacgctgtctatcagcaatcagcgtccgctgttcaccttcgaaaaacatgaggtccctggctacccagacggtcaaaccattgacgaagaaggtaacctgtgggtcgccgtgtttcaaggccagcgtattatcaagattagcacccagcagccggaggttctgctggataccgtcaaaatcccggacccgcaggtgactagcgttgcattcggtggtccgaatctggacgagctgcatgtgaccagcgccggtttgcagctggatgacagctccctggacaaatctttggtcaatggtcacgtctaccgtgtgactggcctgggtgttaagggtttcgccggtgtgaaagttaagctgtaataaggatccttaaggaggtaaaaaaaatggaggacgcgaaaaacatcaagaaaggcccagcaccgttctatccgctggaagatggtaccgccggtgaacaattgcacaaagcgatgaaacgttatgcgctggttccgggtaccatcgcattcacggacgcgcatatcgaggttaacatcacctatgccgagtacttcgagatgagcgttcgtctggccgaggcaatgaagcgttatggtttgaataccaatcatcgcatcgtcgtctgtagcgagaacagcttgcagtttttcatgccagttctgggtgcactgtttattggtgtggcagtggcgcctgcgaatgatatctacaacgagcgtgaactgttgaatagcatgaacatttcgcagccgacggttgtttttgtgtccaagaaaggtttgcagaagattctgaatgtgcaaaagaagttgccgatcattcagaaaatcatcatcatggacagcaagaccgattaccagggtttccaaagcatgtacacttttgtgacgagccacctgcctccgggttttaacgagtatgatttcgttccggaaagcttcgaccgtgataagacgattgcactgattatgaactcgtccggctctaccggcctgcctaaaggcgtggccctgccgcaccgtactgcatgtgtccgcttcagccacgcgcgtgatccgatcttcggtaaccaaatcattccggacaccgccattttgtctgtcgttccgtttcatcatggctttggtatgtttaccactctgggttacctgatctgtggtttccgtgttgttctgatgtaccgtttcgaagaagaactgttcctgcgtagcctgcaagattacaagatccaatccgcgctgctggtgccgacgctgtttagcttcttcgctaaatccaccctgattgacaaatatgacttgagcaacctgcacgaaatcgcgtcgggcggtgcgccgctgagcaaagaagtcggtgaggccgttgcgaaacgtttccatctgccgggcatccgtcagggttatggcctgacggaaacgaccagcgcgattttgattacccctgagggtgacgataaaccgggtgccgttggcaaggtggtaccgttctttgaggctaaagtcgtggacctggacacgggtaagactctgggtgtgaatcaacgtggcgagttgtgcgttcgtggcccgatgatcatgagcggttatgtcaacaacccggaagcaactaatgcgttgatcgataaggatggctggctgcacagcggcgacctggcgtactgggatgaggatgaacactttttcatcgttggtcgcctgaagagcctgatcaagtacaagggctaccaggtggcaccggctgaactggagagcattctgttgcaacaccctaatatcttcgacgcgggtgttgcgggtctgcctgatgatgatgccggtgaattgccagctgcggtggtcgttctggaacacggtaaaacgatgactgaaaaagaaatcgtggactacgtcgcctcgcaggtgaccaccgctaaaaagttgcgtggtggcgtggtgtttgtggatgaagtgccgaaaggcctgacgggtaaacgtgatgcacgtaagattcgtgagatcttgatcaaggcgaagaaaggtggtaaatccaagttgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z