LOCUS       BBa_K990005             2915 bp    DNA     linear       29-Nov-2024
DEFINITION  LasR Device + T4 Lysis Device
ACCESSION   BBa_K990005
VERSION     BBa_K990005.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTetR
     RBS             55..75
                     /label=RBS
     misc_feature    76..824
                     /label=LasR
     misc_feature    825..954
                     /label=Double Terminator
     promoter        955..1122
                     /label=pLas
     misc_feature    1..1122
                     /label=BBa_K772004
     CDS             1147..1803
                     /label=T4 holin
     CDS             1131..1806
                     /label=BBa_K112805
     CDS             1831..2325
                     /label=T4 Endolysin
     CDS             1815..2328
                     /label=BBa_K112806
     stem_loop       2348..2391
                     /label=stem_loop
     misc_feature    2337..2416
                     /label=BBa_B0010
     terminator      2337..2416
                     /label=BBa_B0010
     stem_loop       2432..2451
                     /label=T7 TE
     misc_feature    2458..2458
                     /label=stop
     polyA_site      2452..2465
                     /label=polya
     misc_feature    2425..2465
                     /label=BBa_B0012
     terminator      2425..2465
                     /label=BBa_B0012
     promoter        2474..2508
                     /label=BBa_J23116
     CDS             2531..2824
                     /label=T4 antiholin
     CDS             2517..2827
                     /label=BBa_K112807
     stem_loop       2847..2890
                     /label=stem_loop
     misc_feature    2836..2915
                     /label=BBa_B0010
     terminator      2836..2915
                     /label=BBa_B0010
     misc_feature    1131..2915
                     /label=BBa_K112808
ORIGIN
        1 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt
      121 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag
      181 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc
      241 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg
      301 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag
      361 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg
      421 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg
      481 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc
      541 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg
      601 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt
      661 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat
      721 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg
      781 ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta ataaccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 atagcccctc gctgagcgcg tcccggagct gggggcaacc tagctgccac ctgcttttct
     1021 gctagctatt ccagcgaaaa catacagatt tccggcgaaa tcaaggctac ctgccagttc
     1081 tggcaggttt ggccgcgggt tctttttggt acacgaaagc tatactagag cttaaaagga
     1141 gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt tggtgttctc
     1201 gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt agctgtcgta
     1261 attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt tgagtactat
     1321 aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac tgcacgcttt
     1381 gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc agactttagt
     1441 gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat agcatacgaa
     1501 ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt tgataaaact
     1561 atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc aaaatttgct
     1621 tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg tccatatttt
     1681 aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa tgatcatata
     1741 agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg aagggctaaa
     1801 taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt acgtatagat
     1861 gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat tggcatcggt
     1921 catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga taaagctatt
     1981 gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt taatcaggat
     2041 gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt ttatgattct
     2101 cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg agaaaccggt
     2161 gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga tgaagcagca
     2221 gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa acgagtcatt
     2281 acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta ctagagccag
     2341 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2401 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2461 ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta ctagaggata
     2521 ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat tgtcatttgt
     2581 tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta tggaatctgg
     2641 tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg aacaattcta
     2701 tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg aatgtataga
     2761 gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat tggagactga
     2821 atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2881 ttcgttttat ctgttgtttg tcggtgaacg ctctc
//