BBa_K993003 1 BBa_K993003 Urease generator with ROSE thermometer 2013-06-13T11:00:00Z 2015-05-08T01:13:51Z AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGAGATAAACGCGGGGAGGGAGAAGACGACGATAAGGGTGAGCAACACGGGGGACAGGCCGATACAGGTGGGGAGCCACATACACTTCGTGGAGGTGAACAAGGAGCTGCTGTTCGACAGGGCGGAGGGGATAGGGAGGAGGCTGAACATACCGAGCGGGACGGCGGCGAGGTTCGAGCCGGGGGAGGAGATGGAGGTGGAGCTGACGGAGCTGGGGGGGAACAGGGAGGTGTTCGGGATAAGCGACCTGACGAACGGGAGCGTGGACAACAAGGAGCTGATATTACAAAGGGCGAAGGAGCTGGGGTACAAGGGGGTGGAGTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCATCTTAATCCCGCCGAAAAAGAAAAATTACAAATCTTTCTTGCCTCAGAACTTCTTCTTCGACGAAAAGCCCGAGGCCTTAAACTTAATTATCCCGAAGCCGTCGCCATCATCACCTCATTTATCATGGAAGGCGCCCGAGATGGCAAAACCGTCGCCATGCTTATGGAAGAAGGCAAACATGTCCTTACCCGAGATGATGTCATGGAAGGCGTCCCCGAAATGATCGATGATATCCAAGCCGAAGCCACCTTTCCCGATGGCACCAAGTTGGTCACCGTCCATAATCCCATCTCATAA Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5] false false _1290_ 0 11155 9 Not in stock false Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously. false FURKAN SACİT CEYLAN annotation2329461 1 RBS range2329461 1 1919 1941 annotation2329465 1 RBS range2329465 1 2338 2360 annotation2329459 1 Urease A range2329459 1 194 1903 annotation2329463 1 Urease B range2329463 1 1942 2322 annotation2329468 1 Start Codon range2329468 1 2361 2363 annotation2329460 1 Insulator range2329460 1 1904 1918 annotation2329447 1 pVeg2 range2329447 1 1 97 annotation2329471 1 Stop Codon range2329471 1 2661 2663 annotation2329469 1 Stop Codon range2329469 1 1901 1903 annotation2329470 1 Stop Codon range2329470 1 2320 2322 annotation2329464 1 Insulator range2329464 1 2323 2337 annotation2329467 1 Start Codon range2329467 1 1942 1944 annotation2329466 1 Urease C range2329466 1 2361 2663 annotation2329462 1 Start Codon range2329462 1 194 196 annotation2329458 1 ROSE Thermometer range2329458 1 98 193 BBa_K993003_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttttttgacaaaaatgggctcgtgttgtacaataaatgtgccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggatatgaagataaacaggcagcagtacgcggagagctacgggccgacggtgggggacagggtgaggctggcggacacggacctgggggaggtggagaaggactactactacctgggggacgaggtgaacttcgggggggggaaggtgctgagggaggggatgggggagaacgggacgtacacgaggacggagaacgtgttggacctgctgctgacgaacgcgctgatattggactacacggggatatacaaggcggacataggggtgaaggacgggtacatagtggggatagggaagggggggaacccggacataatggacggggtgacgccgaacatgatagtggggacggcgacggaggtgatagcggcggaggggaagatagtgacggcgggggggatagacacgcacgtgcacttcataaacccggaccaggtggacgtggcgctggcgaacgggataacgacgctgttcgggggggggacggggccggcggaggggagcaaggcgacgacggtgacgccggggccgtggaacatagagaagatgctgaagagcacggaggggctgccgataaacgtggggatactggggaaggggcacgggagcagcatagcgccgataatggagcagatagacgcgggggcggcggggctgaagatacacgaggactggggggcgacgccggcgagcatagacaggagcctgacggtggcggacgaggcggacgtgcaggtggcgatacacagcgacacgctgaacgaggcggggttcttggaggacacggtgagggcgataaacgggagggtgatacacagcttccacgtggagggggcggggggggggcacgcgccggacataatggcgatggcggggcacccgaacgtgctgccgagcagcacgaacccgacgaggccgttcacggtgaacacgatagacgagcacttggacatgctgatggtgtgccaccacctgaagcagaacataccggaggacgtggcgttcgcggacagcaggataaggccggagacgatagcggcggaggacatactgcacgacctggggataataagcatgatgagcacggacgcgctggcgatggggagggcgggggagatggtgctgaggacgtggcagacggcggacaagatgaagaagcagagggggccgctggcggaggagaagaacgggagcgacaacttcaggctgaagaggtacgtgagcaagtacacgataaacccggcgatagcgcaggggatggcgcacgaggtggggagcatagaggaggggaagttcgcggacctggtgctgtgggagccgaagttcttcggggtgaaggcggacagggtgataaagggggggataatagcgtacgcgcagataggggacccgagcgcgagcataccgacgccgcagccggtgatggggaggaggatgtacgggacggtgggggacctgatacacgacacgaacataacgttcatgagcaagagcagcatacagcagggggtgccggcgaagctggggctgaagaggaggatagggacggtgaagaattgtcgaaacatagggaagaaggacatgaagtggaacgacgtgacgacggacatagacataaacccggagacgtacgaggtgaaggtggacggggaggtgctgacgtgcgagccggtgaaggagctgccgatggcgcagaggtacttcctgttctaatcgctgggacgcccgcccctagaaaaggaggactaaaaatgagcaacaacaactacatagtgccgggggagtacagggtggcggagggggagatagagataaacgcggggagggagaagacgacgataagggtgagcaacacgggggacaggccgatacaggtggggagccacatacacttcgtggaggtgaacaaggagctgctgttcgacagggcggaggggatagggaggaggctgaacataccgagcgggacggcggcgaggttcgagccgggggaggagatggaggtggagctgacggagctgggggggaacagggaggtgttcgggataagcgacctgacgaacgggagcgtggacaacaaggagctgatattacaaagggcgaaggagctggggtacaagggggtggagtaatcgctgggacgcccgcccctagaaaaggaggactaaaaatgcatcttaatcccgccgaaaaagaaaaattacaaatctttcttgcctcagaacttcttcttcgacgaaaagcccgaggccttaaacttaattatcccgaagccgtcgccatcatcacctcatttatcatggaaggcgcccgagatggcaaaaccgtcgccatgcttatggaagaaggcaaacatgtccttacccgagatgatgtcatggaaggcgtccccgaaatgatcgatgatatccaagccgaagccacctttcccgatggcaccaagttggtcaccgtccataatcccatctcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z