BBa_M10014
1
BBa_M10014
{˂eCPX!˃} - circularly permuted OmpX
2009-05-09T11:00:00Z
2015-05-08T01:13:51Z
1) Native C-terminal portion of ompX {N.ompX}
PCR Ovu015/Ovu016 on MG1655 gen. (322bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9145-M10012 {N.cpx}
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Ovu015 Construction of OmpX N term part
ctagaGAATTCatgAGATCTGGTCAGTCTggtgactacaacaaaaaccag
Ovu016 Construction of OmpX N term part
gacaaGGATCCgaagcggtaaccaacagaggcaatccaggtgcctac
2) Native N-terminal portion of ompX {C.ompX}
PCR Ovu017/Ovu018 on MG1655 gen. (196bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9145-M10013 {C.cpx}
-----------------------------------------------------------
Ovu017 Construction of OmpX C term part
ctagaGAATTCatgAGATCTgcgacttctactgtaactgg
Ovu018 Construction of OmpX C term part
gacaaGGATCCttaagagcttgcagtacggcttttctcgg
3) SOEing assembly of eCPX
PCR Ovu015/Ovu019 on pBca9145-M10012 (334bp, EcoRI/BamHI = A)
PCR Ovu020/Ovu018 on pBca9145-M10013 (194bp, EcoRI/BamHI = B)
PCR Ovu015/Ovu018 on A+B (505bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, L)
Product is pBca9495KC-M10014 {<eCPX!}
-----------------------------------------------------------
Ovu019 SOEing of eCPX
gtcgcTTTAGACTGTTTAGATCCgaagcggtaaccaacag
Ovu020 SOEing of eCPX
GGATCTAAACAGTCTAAAgcgacttctactgtaactgg
What it does - give referneces
----
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
false
false
_271_
0
1696
271
Not in stock
true
No prepro sequence
SOE-ing PCR used
false
Vaibhavi Umesh
BBa_M10014_sequence
1
ggtcagtctggtgactacaacaaaaaccagtactacggcatcactgctggtccggcttaccgcattaacgactgggcaagcatctacggtgtagtgggtgtgggttatggtaaattccagaccactgaatacccgacctacaaacacgacaccagcgactacggtttctcctacggtgcgggtctgcagttcaacccgatggaaaacgttgctctggacttctcttacgagcagagccgtattcgtagcgttgacgtaggcacctggattgcctctgttggttaccgcttcggatctaaacagtctaaagcgacttctactgtaactggcggttacgcacagagcgacgctcagggccaaatgaacaaaatgggcggtttcaacctgaaataccgctatgaagaagacaacagcccgctgggtgtgatcggttctttcacttacaccgagaaaagccgtactgcaagctcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z